| Budget Amount *help |
¥25,350,000 (Direct Cost: ¥19,500,000、Indirect Cost: ¥5,850,000)
Fiscal Year 2019: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2018: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2017: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
|
|---|
| Outline of Final Research Achievements |
In this study, we aimed to develop a versatile method to analyze double helical structures of nucleic acids by using orientation dependence of Forster resonance energy transfer (FRET). First, we developed another FRET pair for analyses of larger complexes of nucleic acids. We also found that energy transfer between identical chromophores can also be used for structural analyses. Damaged DNA like gapped DNA, A-tract and RNA structures were successfully analyzed by using the developed method. Furthermore, we succeeded in analyses of the interaction between DNA and small molecules by using orientation dependence of FRET.
|
