| Budget Amount *help |
¥23,660,000 (Direct Cost: ¥18,200,000、Indirect Cost: ¥5,460,000)
Fiscal Year 2018: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2017: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2016: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Outline of Final Research Achievements |
Cohesin is a ring-shaped, multisubunit ATPase that topologically encircles DNA. The ring complex makes vital contributions to sister chromatid cohesion and global chromosomal organization to regulate numerous chromosomal events including chromosome segregation, DNA repair and transcriptional regulations. How cohesin establishes these chromosomal structures by topological DNA embrace is poorly understood. Here we found that purified cohesin mediates DNA-DNA interactions by single-stranded DNA (ssDNA)-dependent mechanism. Our genetic analyses have also suggested that ssDNA region plays important roles for establishment of sister chromatid cohesion. This study unveiled a novel cohesin’s activity and provides mechanistic insight into establishment of sister chromatid cohesion.
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