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Investigation of spatio-temporal signals in plant cells

Research Project

Project/Area Number 16H06172
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Plant molecular biology/Plant physiology
Research InstitutionNational Institute of Genetics

Principal Investigator

Oda Yoshihisa  国立遺伝学研究所, 新分野創造センター, 准教授 (30583257)

Research Collaborator SASAKI takema  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥25,090,000 (Direct Cost: ¥19,300,000、Indirect Cost: ¥5,790,000)
Fiscal Year 2017: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2016: ¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000)
Keywords細胞壁 / 微小管 / GTPase / ROPGTPase / 細胞形態形成 / 植物
Outline of Final Research Achievements

In this study, we investigated the proteins and their actions in determining the deposition patterns of plant cell walls in xylem vessel cells. We found that two novel microtubule-associated proteins regulate the interaction between microtubules and the plasma membrane, thereby regulate the distribution pattern of microtubules and ROP GTPases beneath the plasma membrane, resulting in formation of distinct patterns of cell walls. There results imply the cell-autonomous determination of cell wall patterns through the interaction between cytoskeletons and ROP GTPases

Academic Significance and Societal Importance of the Research Achievements

多細胞生物の発生では個々の細胞が適切な形態や機能を獲得するプロセスが必須である。植物においては細胞膜直下に並ぶ微小管やアクチン繊維が細胞壁の沈着パターンを適切に制御することにより細胞の成長や分化を支えている。そのためには細胞内での細胞骨格等の構造の配置を時空間的に厳密に制御する必要があるが、その分子的な実態や作用機序は明らかとなっていなかった。本研究ではタンパク質間の相互作用から自律的に細胞内の空間配置が決定される仕組みの一端が見いだされ、多細胞発生の細胞を支える細胞の振る舞いの理解に繋がる成果となった。

Report

(3 results)
  • 2018 Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (27 results)

All 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (19 results) (of which Int'l Joint Research: 1 results,  Invited: 7 results)

  • [Int'l Joint Research] Czech Academy of Sciences/Charles University in Prague(チェコ)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] A Rho-based reaction-diffusion system governs xylem cell wall patterning in metaxylem vessels.2018

    • Author(s)
      Nagashima, Y., Tsugawa, S., Mochizuki, A., Sasaki, T., Fukuda, H., and Oda, Y.
    • Journal Title

      Sci. Rep.

      Volume: 8 Issue: 1 Pages: 11542-11542

    • DOI

      10.1038/s41598-018-29543-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reconstruction of ROP GTPase Domains on the Plasma Membrane in Tobacco Leaves2018

    • Author(s)
      Oda Yoshihisa、Nagashima Yoshinobu、Fukuda Hiroo
    • Journal Title

      Methods Mol Biol

      Volume: - Pages: 393-399

    • DOI

      10.1007/978-1-4939-8612-5_26

    • ISBN
      9781493986118, 9781493986125
    • Related Report
      2017 Annual Research Report
  • [Journal Article] Emerging roles of cortical microtubule-membrane interactions2018

    • Author(s)
      Oda Y
    • Journal Title

      J Plant Res

      Volume: 131 Issue: 1 Pages: 5-14

    • DOI

      10.1007/s10265-017-0995-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CORTICAL MICROTUBULE DISORDERING1 Is Required for Secondary Cell Wall Patterning in Xylem Vessels2017

    • Author(s)
      Sasaki Takema、Fukuda Hiroo、Oda Yoshihisa
    • Journal Title

      Plant Cell

      Volume: 29 Issue: 12 Pages: 3123-3139

    • DOI

      10.1105/tpc.17.00663

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A novel plasma membrane-anchored protein regulates xylem cell-wall deposition through microtubule-dependent lateral inhibition of Rho GTPase domains2017

    • Author(s)
      Sugiyama Y, Wakazaki M, Toyooka K, Fukuda H, Oda Y
    • Journal Title

      Current Biology

      Volume: 27 Issue: 16 Pages: 2522-2528

    • DOI

      10.1016/j.cub.2017.06.059

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microtubule-dependent targeting of the exocyst complex is necessary for xylem development in Arabidopsis.2017

    • Author(s)
      Vukasinovic N, Oda Y, Pejchar P, Synek L, Pecenkova T, Rawat A, Sekeres J, Potocky M, Zarsky V
    • Journal Title

      New Phytologist

      Volume: 213 Issue: 3 Pages: 1052-1067

    • DOI

      10.1111/nph.14267

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] VND6-induced Xylem Cell Differentiation in Arabidopsis Cell Cultures2017

    • Author(s)
      Oda Y
    • Journal Title

      Methods Mol Biol.1544

      Volume: 1544 Pages: 67-73

    • DOI

      10.1007/978-1-4939-6722-3_6

    • ISBN
      9781493967209, 9781493967223
    • Related Report
      2016 Annual Research Report
    • Acknowledgement Compliant
  • [Presentation] 道管の壁孔パターンを制御する ROP GTPase の解析2018

    • Author(s)
      長島慶宜,津川暁,望月敦史,佐々木武馬,福田裕穂,小田祥久
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] IQD13 は微小管と細胞膜に相互作用し、二次細胞壁のパターン形成を制御する2018

    • Author(s)
      杉山友希,福田裕穂,小田祥久
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] CORTICAL MICROTUBULE DISORDERING1 (CORD1) は木部道管細胞において蓄積する細胞壁の構造を制御する2018

    • Author(s)
      佐々木武馬,福田裕穂,小田祥久
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 木部道管分化における自律的な細胞壁パターンの形成2017

    • Author(s)
      小田 祥久
    • Organizer
      MIMS現象数理学拠点共同研究集会「時空間ダイナミクス~生命現象における時間変化を伴う空間秩序」
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 細胞膜ドメインの形を制御する新規の微小管付随タンパク質の解析2017

    • Author(s)
      杉山 友希, 福田 裕穂, 小田 祥久
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 新規木部細胞分化誘導系を用いた二次細胞壁パターン制御機構の解析2017

    • Author(s)
      小田 祥久, 佐々木 武馬
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 表層微小管の脱配向性を促進する新規微小管局在性タンパク質の機能解析2017

    • Author(s)
      佐々木 武馬, 福田 裕穂, 小田 祥久
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 道管において二次細胞壁パターンを制御する協調的なROPシグナル分子の解析2017

    • Author(s)
      長島 慶宜, 福田 裕穂, 小田 祥久
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Moleular basis of secondary cell wall patterning2017

    • Author(s)
      Yoshihisa Oda
    • Organizer
      GDRI Integrative Plant Biology "The Developing Plant in its Environment"
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Secondary cell wall patterning in metaxylem vessels2017

    • Author(s)
      Yoshihisa Oda
    • Organizer
      TAIWAN-JAPAN Plant Biology
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] A novel microtubule-localized protein regulates the structure of xylem vessels2017

    • Author(s)
      Takema Sasaki, Hiroo Fukuda, Yoshihisa Oda
    • Organizer
      The 1st IROAST Symposium: Plant Cell and Developmental Biology: Approaches to Multiscale Biosystems
    • Related Report
      2017 Annual Research Report
  • [Presentation] 道管分化における細胞壁パターン形成2017

    • Author(s)
      小田祥久
    • Organizer
      ConBio2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] PL-046 二次細胞壁のパターン形成において細胞膜ドメインの形は新規の細胞膜‐微小管付随タンパク質により制御される2017

    • Author(s)
      杉山友希,若崎眞由美,佐藤繭子,豊岡公徳,福田裕穂,小田祥久
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 1aD10 シロイヌナズナにおいて道管細胞の構造を制御する新規微小管局在因子の解析2017

    • Author(s)
      佐々木武馬,福田裕穂,小田祥久
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 1aB09 道管において壁孔の形成を協調的に制御するROP GTPase シグナルの解析2017

    • Author(s)
      長島慶宜,福田裕穂,小田祥久
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学、鹿児島市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 2AS12-2 Geometry formation of the immobile plant xylem cell2016

    • Author(s)
      Yoshihisa Oda
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜、横浜市
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] P-1336 シロイヌナズナに おいて細胞の形態を制御する転写因子の探索2016

    • Author(s)
      佐々木武馬, 松井南, 小田祥久
    • Organizer
      日本植物学会第80回沖縄大会
    • Place of Presentation
      沖縄コンベンションセンター、那覇市
    • Related Report
      2016 Annual Research Report
  • [Presentation] 3aSA01 Dissection and reconstruction of cell-wall patterning machinery2016

    • Author(s)
      Yoshihisa Oda
    • Organizer
      JPR International Symposium:Semi-in-vivo developmental biology
    • Place of Presentation
      沖縄コンベンションセンター、那覇市
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 木部道管分化にみる細胞の自立的な空間パターン形成2016

    • Author(s)
      小田祥久
    • Organizer
      国立遺伝学研究所新分野創造センター創立10週年シンポジウム
    • Place of Presentation
      東京大学本郷キャンパス、文京区
    • Related Report
      2016 Annual Research Report
    • Invited

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Published: 2016-04-21   Modified: 2020-03-30  

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