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Dynamic conformation and domain structure of lipid molecules in model biomembranes

Research Project

Project/Area Number 16H06315
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Biomolecular chemistry
Research InstitutionOsaka University

Principal Investigator

MURATA Michio  大阪大学, 理学研究科, 教授 (40183652)

Co-Investigator(Kenkyū-buntansha) 花島 慎弥  大阪大学, 理学研究科, 准教授 (50373353)
梅川 雄一  大阪大学, 理学研究科, 助教 (20587779)
篠田 渉  名古屋大学, 工学研究科, 准教授 (70357193)
土川 博史  大阪大学, 理学研究科, 助教 (30460992)
三浦 薫 (野村薫)  公益財団法人サントリー生命科学財団, 生物有機科学研究所・構造生命科学研究部, 主席研究員 (90353515)
櫻井 香里  東京農工大学, 工学(系)研究科(研究院), 准教授 (50447512)
Project Period (FY) 2016-05-31 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥182,780,000 (Direct Cost: ¥140,600,000、Indirect Cost: ¥42,180,000)
Fiscal Year 2020: ¥31,590,000 (Direct Cost: ¥24,300,000、Indirect Cost: ¥7,290,000)
Fiscal Year 2019: ¥36,660,000 (Direct Cost: ¥28,200,000、Indirect Cost: ¥8,460,000)
Fiscal Year 2018: ¥32,500,000 (Direct Cost: ¥25,000,000、Indirect Cost: ¥7,500,000)
Fiscal Year 2017: ¥35,620,000 (Direct Cost: ¥27,400,000、Indirect Cost: ¥8,220,000)
Fiscal Year 2016: ¥46,410,000 (Direct Cost: ¥35,700,000、Indirect Cost: ¥10,710,000)
Keywords脂質二重膜 / 脂質ラフト / スフィンゴミエリン / ドメイン構造 / 古細菌脂質 / 動的配座
Outline of Final Research Achievements

In this study, we aimed to elucidate the molecular mechanisms of domains formed on cell membranes at atomic resolution in order to bring biological membranes into the ring of structural biology. By chemically synthesizing stable isotope-conjugated membrane lipids and measuring solid-state NMR, they succeeded in elucidating the shape of membrane lipid molecules, the mechanism of their assembly, and the intermolecular interactions during assembly. For example, it was found that domain-forming lipids have a strong tendency to form nano-sized aggregates through intermolecular hydrogen bonds.

Academic Significance and Societal Importance of the Research Achievements

脂質ラフトを含むドメイン形成に必要な、立体配置と分子間相互作用を化学構造の面から解明できれば、膜タンパク質との相互作用時の脂質立体配座や水和構造の違いを正確に知ることができる。これらの脂質の動態は、揺らぎ、配向や分子間相互作用などのパラメータとして定式化できるので、分子動力学(MD)計算と組み合わせることが可能である。これによって、今まで実験的根拠に乏しかった膜脂質の計算科学的方法の精度を格段に向上させることができる。

Assessment Rating
Verification Result (Rating)

A

Assessment Rating
Result (Rating)

A: Progress in the research is steadily towards the initial goal. Expected research results are expected.

Report

(11 results)
  • 2022 Research Progress Assessment (Verification Result) ( PDF )
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Abstract ( PDF )   Comments on the Screening Results ( PDF )   Annual Research Report
  • Research Products

    (112 results)

All 2022 2021 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (7 results) Journal Article (29 results) (of which Int'l Joint Research: 11 results,  Peer Reviewed: 29 results,  Open Access: 6 results,  Acknowledgement Compliant: 1 results) Presentation (69 results) (of which Int'l Joint Research: 6 results,  Invited: 4 results) Remarks (5 results) Funded Workshop (2 results)

  • [Int'l Joint Research] Stony Brook University(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Abo Akademi University(フィンランド)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Åbo Akademi University(フィンランド)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Stony Brook University(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Abo Akademi University(フィンランド)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Stony Brook University(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Abo Akademi University(フィンランド)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] FRET detects lateral interaction between transmembrane domain of EGF receptor and ganglioside GM3 in lipid bilayers2021

    • Author(s)
      Nakano Mikito、Hanashima Shinya、Hara Toshiaki、Kabayama Kazuya、Asahina Yuya、Hojo Hironobu、Komura Naoko、Ando Hiromune、Nyholm Thomas K.M.、Slotte J. Peter、Murata Michio
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Biomembranes

      Volume: 1863 Issue: 8 Pages: 183623-183623

    • DOI

      10.1016/j.bbamem.2021.183623

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] β-Glucosylation of cholesterol reduces sterol-sphingomyelin interactions2021

    • Author(s)
      Hanashima Shinya、Fukuda Nanami、Malabed Raymond、Murata Michio、Kinoshita Msanao、Greimel Peter、Hirabayashi Yoshio
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Biomembranes

      Volume: 1863 Issue: 2 Pages: 183496-183496

    • DOI

      10.1016/j.bbamem.2020.183496

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conformation and Orientation of Branched Acyl Chains Responsible for the Physical Stability of Diphytanoylphosphatidylcholine.2020

    • Author(s)
      Hiroshi Tsuchikawa, Takuya Ono, Masaki Yamagami, Yuichi Umegawa, Wataru Shinoda, Michio Murata
    • Journal Title

      Biochemistry

      Volume: 59 Issue: 40 Pages: 3929-3938

    • DOI

      10.1021/acs.biochem.0c00589

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sphingomyelins and ent-Sphingomyelins Form Homophilic Nano-Subdomains within Liquid Ordered Domains2020

    • Author(s)
      Yano Yo、Hanashima Shinya、Tsuchikawa Hiroshi、Yasuda Tomokazu、Slotte J. Peter、London Erwin、Murata Michio
    • Journal Title

      Biophysical Journal

      Volume: 119 Issue: 3 Pages: 539-552

    • DOI

      10.1016/j.bpj.2020.06.028

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Interactions of OSW-1 with Lipid Bilayers in Comparison with Digitonin and Soyasaponin.2020

    • Author(s)
      Raymond Malabed, Shinya Hanashima, Michio Murata, Kaori Sakurai
    • Journal Title

      Langmuir

      Volume: 36 Issue: 13 Pages: 3600-3610

    • DOI

      10.1021/acs.langmuir.9b03957

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cholesterol-Induced Conformational Change in the Sphingomyelin Headgroup2019

    • Author(s)
      Hanashima Shinya、Murakami Kazuhiro、Yura Michihiro、Yano Yo、Umegawa Yuichi、Tsuchikawa Hiroshi、Matsumori Nobuaki、Seo Sangjae、Shinoda Wataru、Murata Michio
    • Journal Title

      Biophysical Journal

      Volume: 117 Issue: 2 Pages: 307-318

    • DOI

      10.1016/j.bpj.2019.06.019

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Average Conformation of Branched Chain Lipid PGP-Me That Accounts for the Thermal Stability and High-Salinity Resistance of Archaeal Membranes.2019

    • Author(s)
      Yamagami, M., Tsuchikawa, H., Cui, J., Umegawa, Y., Miyazaki, Y., Seo, S., Shinoda, W., Murata, M.
    • Journal Title

      Biochemistry

      Volume: 58 Issue: 37 Pages: 3869-3879

    • DOI

      10.1021/acs.biochem.9b00469

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Side-chain deuterated cholesterol as a molecular probe to determine membrane order and cholesterol partitioning2019

    • Author(s)
      Hanashima Shinya、Ibata Yuki、Watanabe Hirofumi、Yasuda Tomokazu、Tsuchikawa Hiroshi、Murata Michio
    • Journal Title

      Organic & Biomolecular Chemistry

      Volume: 17 Issue: 37 Pages: 8601-8610

    • DOI

      10.1039/c9ob01342c

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Amphotericin B-Ergosterol Complex Spans a Lipid Bilayer as a Single-Length Assembly.2019

    • Author(s)
      Tomoya Yamamoto, Yuichi Umegawa, Hiroshi Tsuchikawa, Shinya Hanashima, Nobuaki Matsumori, Kosuke Funahashi, Sangjae Seo, Wataru Shinoda, Michio Murata.
    • Journal Title

      Biochemistry

      Volume: 58 Issue: 51 Pages: 5188-5196

    • DOI

      10.1021/acs.biochem.9b00835

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of 7,6-Spirocyclic Imine with Butenolide Ring Provides Evidence for the Relative Configuration of Marine Toxin 13-desMe Spirolide C.2019

    • Author(s)
      Kou Minamino, Michio Murata, Hiroshi Tsuchikawa.
    • Journal Title

      Organic Letters

      Volume: 21 Issue: 22 Pages: 8970-8975

    • DOI

      10.1021/acs.orglett.9b03373

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of Acyl Chain Mismatch on the Formation and Properties of Sphingomyelin-Cholesterol Domains.2019

    • Author(s)
      Nyholm, Thomas K. M.; Engberg, Oskar; Hautala, Victor; Tsuchikawa, Hiroshi; Lin, Kai-Lan; Murata, Michio; Slotte, J. Peter
    • Journal Title

      Biophys. J.

      Volume: 117 Issue: 9 Pages: 1577-1588

    • DOI

      10.1016/j.bpj.2019.09.025

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Theonellamide A, a marine-sponge-derived bicyclic peptide, binds to cholesterol in aqueous DMSO: Solution NMR-based analysis of peptide-sterol interactions using hydroxylated sterol2019

    • Author(s)
      Cornelio Kimberly、Espiritu Rafael Atillo、Hanashima Shinya、Todokoro Yasuto、Malabed Raymond、Kinoshita Masanao、Matsumori Nobuaki、Murata Michio、Nishimura Shinichi、Kakeya Hideaki、Yoshida Minoru、Matsunaga Shigeki
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Biomembranes

      Volume: 1861 Issue: 1 Pages: 228-235

    • DOI

      10.1016/j.bbamem.2018.07.010

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] SPICA Force Field for Lipid Membranes: Domain Formation Induced by Cholesterol.2019

    • Author(s)
      Seo, S., Shinoda, W.
    • Journal Title

      J. Chem. Theory Comput.

      Volume: 15 Issue: 1 Pages: 762-774

    • DOI

      10.1021/acs.jctc.8b00987

    • NAID

      120006623810

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Small structural alterations greatly influence the membrane affinity of lipophilic ligands: Membrane interactions of bafilomycinA1 and its desmethyl derivative bearing 19F-labeling.2019

    • Author(s)
      Tatsuru Hayashi, Hiroshi Tsuchikawa, Yuichi Umegawa, Michio Murata.
    • Journal Title

      Bioorg. Med. Chem.

      Volume: 27 Issue: 8 Pages: 1677-1682

    • DOI

      10.1016/j.bmc.2019.03.017

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Perpendicular Orientation of Amphotericin B Methyl Ester in Hydrated Lipid Bilayers Supports the Barrel-Stave Model2019

    • Author(s)
      Yamamoto Tomoya、Umegawa Yuichi、Yamagami Masaki、Suzuki Taiga、Tsuchikawa Hiroshi、Hanashima Shinya、Matsumori Nobuaki、Murata Michio
    • Journal Title

      Biochemistry

      Volume: 印刷中 Issue: 17 Pages: 2282-2291

    • DOI

      10.1021/acs.biochem.9b00180

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nanosized phase segregation of sphingomyelin and dihydrosphigomyelin in unsaturated phosphatidylcholine binary Membranes without Cholesterol.2018

    • Author(s)
      Yasuda, T., Slotte, J. P., Murata, M.
    • Journal Title

      Langmuir

      Volume: 34 Issue: 44 Pages: 13426-13437

    • DOI

      10.1021/acs.langmuir.8b02637

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Stereoselective Construction of Cisoidal Bisspiroacetal Frameworks through Magnesium Coordination of the Bilateral Acetal Oxygen Atoms2018

    • Author(s)
      Yoshifumi Yasukawa, Hiroshi Tsuchikawa, Yasuto Todokoro, and Michio Murata
    • Journal Title

      Asian Journal of Organic Chemistry

      Volume: - Issue: 6 Pages: 1101-1106

    • DOI

      10.1002/ajoc.201800074

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sphingomyelin Stereoisomers Reveal That Homophilic Interactions Cause Nanodomain Formation2018

    • Author(s)
      Yano Yo、Hanashima Shinya、Yasuda Tomokazu、Tsuchikawa Hiroshi、Matsumori Nobuaki、Kinoshita Masanao、Al Sazzad Md. Abdullah、Slotte J. Peter、Murata Michio
    • Journal Title

      Biophysical Journal

      Volume: 115 Issue: 8 Pages: 1530-1540

    • DOI

      10.1016/j.bpj.2018.08.042

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Highly efficient syntheses of C-N axially chiral 1‐(ortho‐hydroxyaryl)uracil using a chiral auxiliary and a chiral base.2018

    • Author(s)
      Hasegawa, F., Yasukawa, Y., Kawamura, K., Tsuchikawa, H., Murata. M.
    • Journal Title

      Asian J. Org. Chem.

      Volume: 7 Issue: 8 Pages: 1648-1653

    • DOI

      10.1002/ajoc.201800247

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sterol-recognition ability and membrane-disrupting activity of Ornithogalum saponin OSW-1 and usual 3- O -glycosyl saponins2017

    • Author(s)
      Malabed Raymond、Hanashima Shinya、Murata Michio、Sakurai Kaori
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1859 Issue: 12 Pages: 2516-2525

    • DOI

      10.1016/j.bbamem.2017.09.019

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Emphatic visualization of sphingomyelin-rich domains by inter-lipid FRET imaging using fluorescent sphingomyelins2017

    • Author(s)
      1.Kinoshita M, Ano H, Murata M, Shigetomi K, Ikenouchi J, Matsumori N
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 16801-16801

    • DOI

      10.1038/s41598-017-16361-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bilayer Interactions among Unsaturated Phospholipids, Sterols, and Ceramide2017

    • Author(s)
      Slotte J. Peter、Yasuda Tomokazu、Engberg Oskar、Al Sazzad Md. Abdullah、Hautala Victor、Nyholm Thomas K.M.、Murata Michio
    • Journal Title

      Biophysical Journal

      Volume: 112 Issue: 8 Pages: 1673-1681

    • DOI

      10.1016/j.bpj.2017.03.016

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs2017

    • Author(s)
      Masanao Kinoshita, Kenichi GN Suzuki, Nobuaki Matsumori, Misa Takada, Hikaru Ano, Kenichi Morigaki, Mitsuhiro Abe, Asami Makino, Toshihide Kobayashi, Koichiro M Hirosawa, Takahiro K Fujiwara, Akihiro Kusumi, Michio Murata
    • Journal Title

      J Cell Biol

      Volume: 未定 Issue: 4 Pages: 1183-1204

    • DOI

      10.1083/jcb.201607086

    • NAID

      120005998045

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Nanosecond pump probe device for time-resolved serial femtosecond crystallography developed at SACLA2017

    • Author(s)
      Kubo Minoru、Nango Eriko、Murata Michio、Neutze Richard、Yabashi Makina、Iwata So, その他18名
    • Journal Title

      Journal of Synchrotron Radiation

      Volume: 24 Issue: 5 Pages: 1086-1091

    • DOI

      10.1107/s160057751701030x

    • NAID

      120006363292

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 13C-TmDOTA as versatile thermometer compound for solid-state NMR of hydrated lipid bilayer membranes2016

    • Author(s)
      Umegawa Yuichi、Tanaka Yuya、Matsumori Nobuaki 、Murata Michio
    • Journal Title

      Magnetic Resonance in Chemistry

      Volume: 54 Issue: 3 Pages: 227-233

    • DOI

      10.1002/mrc.4371

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lipid Interactions and Organization in Complex Bilayer Membranes2016

    • Author(s)
      Engberg, O; Yasuda, T; Hautala, V; Matsumori, N; Nyholm, TKM; Murata, M; Slotte, JP
    • Journal Title

      BIOPHYSICAL JOURNAL

      Volume: 110 Issue: 7 Pages: 1563-1573

    • DOI

      10.1016/j.bpj.2015.12.043

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Structure of the Bimolecular Complex between Amphotericin B and Ergosterol in Membranes Is Stabilized by Face-to-Face van der Waals Interaction with Their Rigid Cyclic Cores2016

    • Author(s)
      Yasuo Nakagawa, Yuichi Umegawa, Naohiro Matsushita, Tomoya Yamamoto, Hiroshi Tsuchikawa, Shinya Hanashima, Tohru Oishi, Nobuaki Matsumori, Michio Murata
    • Journal Title

      Biochemistry

      Volume: 55 Issue: 24 Pages: 3392-3402

    • DOI

      10.1021/acs.biochem.6b00193

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A three dimensional movie of structural changes in bacteriorhodopsin.2016

    • Author(s)
      Nango, E., Royant, A.†, Kubo, M.† et al. (†equal contribution)(45名中2番目)
    • Journal Title

      Science

      Volume: 354 Issue: 6319 Pages: 1552-1557

    • DOI

      10.1126/science.aah3497

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Evaluation of diacylphospholipids as boundary lipids for bacteriorhodopsin from structural and functional aspects2016

    • Author(s)
      Kawatake, S., Umekawa, Y., Matsuoka, S., Murata, M., Sonoyama, M.
    • Journal Title

      Biochim. Biophys. Acta

      Volume: 1858 Issue: 9 Pages: 2106-2115

    • DOI

      10.1016/j.bbamem.2016.06.006

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] 表裏の脂質構成が異なる脂質二重層におけるコリン脂質の内外葉比の定量的評価2022

    • Author(s)
      渡辺宏史、花島慎弥、安田智一、村田道雄
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ガングリオシドGM3糖鎖とインテグリンの相互作用のSPR解析2022

    • Author(s)
      岸 水際、花島 慎弥、村田 道雄、鈴木 健一
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 固体NMRによる脂質二重膜におけるリン脂質不飽和脂肪鎖の配座・配向解析2022

    • Author(s)
      安田 貴也、梅川 雄一、篠田 渉、村田 道雄
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 生体モデル膜におけるSrcファミリーキナーゼLyn脂質アンカー鎖の動態解析2022

    • Author(s)
      水戸 佳奈子、花島 慎弥、梅川 雄一、村田 道雄、北條 裕信
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Investigation of Inner Leaflet Lipids Influence on Sphingomyelin Domain2022

    • Author(s)
      Liao, C-H., Hanashima, S., Yasuda, T., Murata, M.
    • Organizer
      日本化学会第102春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 膜タンパク質および周辺リン脂質二重膜の深度依存的な水和状態の評価2021

    • Author(s)
      髙地 愛、梅川 雄一、村田 道雄
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 表裏の脂質組成の異なる二重膜リポソームにおけるコレステロール内外葉分布の解明2021

    • Author(s)
      渡辺 宏史、花島 慎弥、村田 道雄
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 水和脂質膜の固体NMR測定による高度不飽和脂肪酸結合リン脂質の分子機構の解明2021

    • Author(s)
      大田英和、梅川雄一、花島慎弥、土川 博史、村田 道雄
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 重水素固体NMRを用いた相分離二重膜中のLo/Ld相脂質組成の定量的解析2021

    • Author(s)
      山中 彩菜、花島 慎弥、井端 悠貴、梅川 雄一、村田 道雄
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 蛍光スフィンゴ脂質プローブを用いたモデル膜中でのスフィンゴ脂質ドメイン構造のFRET解析2021

    • Author(s)
      友田 千尋、矢野 陽、花島 慎弥、河村 奈緒子、安藤 弘宗、村田 道雄、London, E.
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ガングリオシドGM3糖鎖と結合タンパク質の親和性を制御する脂質間相互作用の解明2021

    • Author(s)
      佐々木 克聡、花島 慎弥、梅川 雄一、村田 道雄
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] EGF受容体膜貫通ドメインとガングリオシドGM3の側方相互作用のFRET解析における膜脂質の影響評価2021

    • Author(s)
      中野 幹人、花島 慎弥、原 利明、樺山 一哉、河村 奈緒子、安藤 弘宗、Nyholm, T., Slotte, P., 村田 道雄
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Membrane interaction and disrupting activity of the triterpenoid saponin ginsenoside Rh2 to cholesterol and sphingomyelin-containing liposomes2021

    • Author(s)
      Garza, D, L., Murata, M., Hanashima, S.
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Elucidation of inter-leaflet coupling in asymmetric membranes induced by very long chain sphingomyelin based on solid-state NMR2021

    • Author(s)
      Keeratisakulsith, T., Umegawa, Y., Tsuchikawa, H., Hanashima, S., Murata, M., Shinoda, W.
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Diosgenin-induced physicochemical effects on phospholipid bilayers in comparison with Cholesterol2021

    • Author(s)
      Ondevilla, J. C., Hanashima, S., Umegawa, Y., Murata, M.
    • Organizer
      日本化学会第101春季年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Cholesterol effect on the membrane activities of the diosgenyl saponins2020

    • Author(s)
      ONDEVILLA, Joan Candice; HANASHIMA, Shinya; MURATA, Michio
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] リン固体NMRとCD測定を用いたバクテリオロドプシンと周辺脂質の相互作用解析2020

    • Author(s)
      加藤翔,村田道雄
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Evaluation of lateral interactions between membrane gangliosides and receptor transmembrane segments in lipid bilayers based on semi-quantitative fluorescence analysis2020

    • Author(s)
      NAKANO, Mikito; HANASHIMA, Shinya; HARA, Toshiaki; KABAYAMA, Kazuya; FUKASE, Koichi; MURATA, Michio; ANDO, Hiromune; SUZUKI, Kenichi G. N.; SLOTTE, J. Peter
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 固体NMRによる脂質二重膜中におけるアンフォテリシンBの会合状態の解明2020

    • Author(s)
      鈴木大河、梅川雄一、村田道雄
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 位置選択的標識化脂質を用いた固体NMRによる内葉構成リン脂質の膜物性解析2020

    • Author(s)
      向川茜、土川博史、梅川雄一、村田道雄
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 固体NMRによる脂質二重膜におけるスフィンゴミエリン側鎖の配座・配向解析2020

    • Author(s)
      今井茅希、村田道雄、梅川 雄一
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 脂質膜上で形成されるラクトシルセラミドの強固なドメインの固体NMR解析2020

    • Author(s)
      松原侑輝、池田竜二、花島慎弥、村田 道雄
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] スフィンゴミエリン蛍光プローブのFRET測定を用いたモデル膜における脂質ナノドメイン構造の解明2020

    • Author(s)
      友田千尋、矢野陽、花島慎弥、村田道雄、London Erwin
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Evaluation of hydration state of protein-lipid interface by using fluorescence probes2020

    • Author(s)
      KOCHI, Ai; UMEGAWA, Yuichi; MURATA, Michio
    • Organizer
      日本化学会 第100春季年会 (2020)
    • Related Report
      2019 Annual Research Report
  • [Presentation] 重水素標識化脂質を用いた流動相における相分離二重膜の精密 NMR 解析2019

    • Author(s)
      山中 彩菜, 井端 悠貴, 土川 博史, 花島 慎弥, 村田 道雄
    • Organizer
      第9回CSJ化学フェスタ2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 表裏の脂質組成が異なる二重膜リポソームの調製と表裏の秩序化の観測2019

    • Author(s)
      渡辺宏史・矢野陽・花島慎弥・安田智一・村田道雄・Erwin London
    • Organizer
      第9回CSJ化学フェスタ2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 水和脂質膜中での高度不飽和脂肪酸脂質の運動性解析2019

    • Author(s)
      大田英和, 梅川雄一, 花島慎弥, 土川博史, 村田道雄
    • Organizer
      第59回NMR討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 脂質二重膜での分子間相互作用とドメイン形成2019

    • Author(s)
      村田道雄
    • Organizer
      応用物理学会・シンポジウム「ナノバイオ分野での実験と計算の連携・融合:脂質膜と膜タンパク質」
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 膜脂質の構造・動態および膜タンパク質との相互作用2019

    • Author(s)
      村田道雄
    • Organizer
      日本学術振興会回折構造生物第169委員会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Synthesis of novel 13C, 2H-labeled Amphotericin B and the conformational analysis of glycosidic linkage in lipid bilayers.2019

    • Author(s)
      Yamamoto, T., Umegawa, Y., Suzuki, T., Tsuchikawa, H., Hanashima, S., Murata, M., Matsumori, N., Seo, S., Funahashi, K., Shinoda, W.
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Interaction of ganglioside with the EGF receptor transmembrane segments in model membrane.2019

    • Author(s)
      Nakano, M., Hanashima, S., Hara, T., Murata, M., Kabayama, K., Fukase, K., Ando, H., Suzuki, K., Slotte, P.
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 非対称膜におけるスフィンゴミエリンとコレステロールの脂質間相互作用の固体NMR解析2019

    • Author(s)
      矢野陽、花島慎弥、土川博史、村田道雄、London Erwin
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脂質膜環境における糖鎖の配向解析を目指したガングリオシドGM3頭部重水素標識体の合成2019

    • Author(s)
      佐々木克聡、花島慎弥、村田道雄
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] メチル分岐アシル基含有リン脂質DPhPCが示す二重膜の安定性を担う分子配向・配座の解析2019

    • Author(s)
      斧拓冶、土川博史、山上正輝、梅川雄一、村田道雄、篠田渉
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 混合モデル二重膜における極長鎖スフィンゴミエリンの内葉・外葉間相互作用の解明2019

    • Author(s)
      足立美和子、森内舞、土川博史・Seo Sangjae、篠田渉、村田道雄
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 新規コレステロール側鎖重水素標識体の開発とそれを用いた三成分膜での相状態の解明2019

    • Author(s)
      井端悠貴、花島慎弥、安田智一、土川博史、村田道雄
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脂質膜中ドメイン形成の機構解明を目指したスフィンゴミエリンのNMR緩和時間による回転相関時間の測定とクラスターサイズの推定2019

    • Author(s)
      木下雄哉、梅川雄一、土川博史、村田道雄
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 脳内新規糖脂質コレステリル-β-グルコシドのモデル膜における脂質間相互作用の解明2019

    • Author(s)
      福田奈那美、花島慎弥、Malabed Raymond、村田道雄、Greimel Peter、平林義雄
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 位置選択的重水素標識体を用いた内葉構成リン脂質の固体NMRによる脂質間相互作用の解明2019

    • Author(s)
      向川茜、土川博史、村田道雄
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 固体リンNMRを用いたバクテリオロドプシンと周辺脂質の相互作用解析2019

    • Author(s)
      加藤翔、梅川雄一、村田道雄、Seo Sangjae、篠田渉
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 混合脂質モデル膜におけるラクトシルセラミドの強固なドメインの固体NMR解析2019

    • Author(s)
      松原侑輝、花島慎弥、池田竜二、村田道雄
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 2H,13C-二重標識スフィンゴミエリンを用いた脂質二重膜におけるアシル鎖の配座・配向の固体NMR解析2019

    • Author(s)
      今井茅希、梅川雄一、土川博史、村田道雄
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] アミロイドペプチドへの結合モチーフとしての各種梯子状ポリエーテル化合物の評価と相互作用についての考察2019

    • Author(s)
      Xiong Weiqi、土川博史、原利明、村田道雄、佐竹真幸
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Amphotericin B チャネルの構造安定性と疎水性ミスマッチによるチャネル間相互作用解析2018

    • Author(s)
      舟橋康佑, Sangjae Seo、岡崎進、梅川雄一、村田道雄、篠田渉
    • Organizer
      第32回分子シミュレーション討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] アンフォテリシンBとステロールが形成するチャネル複合体の構造研究2018

    • Author(s)
      山本智也、梅川雄一、中川泰男、 鈴木大河、山上正輝、土川博史、花島慎弥、村田道雄、松森信明、Seo Sangjaed、篠田渉
    • Organizer
      第60回天然有機化合物討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Molecular dynamics simulations of domain formation in mixed lipid bilayers.2018

    • Author(s)
      Seo, S., Shinoda, W.
    • Organizer
      第56回日本生物物理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 特異な膜挙動を示す極長鎖スフィンゴミエリンの混合モデル膜中における運動性解析2018

    • Author(s)
      足立美和子、森内舞、土川博史、村田道雄
    • Organizer
      日本ケミカルバイオロジー学会第十三回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Membrane behavior of very long chain sphingomyelin in lipid bilayers deduced from solid state NMR.2018

    • Author(s)
      Tsuchikawa, H., Moriuchi, M., Adachi, M., Murata, M., Slotte, J. P.
    • Organizer
      International Workshop on Biological Membranes
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of Inhibitory Mechanism of Bafilomycin against Vacuolar-type ATPase by Solid-state NMR.2018

    • Author(s)
      Tsuchikawa, H.
    • Organizer
      2018 GRC-Osaka U Bilateral Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Molecular Dynamics Study of Stability of Amphotericin B Channel.2018

    • Author(s)
      Funahashi, K., Seo, S., Okazaki, S., Umegawa, Y., Murata, M., Shinoda. W.
    • Organizer
      Conference of EMLG/JMLG Meeting 2018 and 41st Symposium on Solution Chemistry of Japan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Coarse-grained modeling of sphingomyelin and domain formation simulation.2018

    • Author(s)
      Seo, S., Shinoda, W.
    • Organizer
      Joint Conference of EMLG/JMLG Meeting 2018 and 41st Symposium on Solution Chemistry of Japan
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cholesterol Interaction with Steroidal Saponins in Bilayer Membrane.2018

    • Author(s)
      Hanashima, S., Malabed R., Murata M.
    • Organizer
      ICPAC2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sterol-dependent membrane activity of steroidal saponin OSW-1 using spectroscopic and microscopic approaches2018

    • Author(s)
      Malabed Raymond、Hanashima Shinya、Murata Michio、Sakurai Kaori
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Analysis of the raft-like nanodomains using sphingomyelin antipode2018

    • Author(s)
      Yano Yo、Hanashima Shinya、Yasuda Tomokazu、Tsuchikawa Hiroshi、Murata Michio、Kinoshita Masanao、Matsumori Nobuaki、Suzuki Kenichi、J. Peter. Slotte、Erwin London
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Cholesterol Interaction with Steroidal Saponins in Bilayer Membrane2018

    • Author(s)
      Hanashima Shinya、Malabed Raymond、Murata Michio
    • Organizer
      ICPAC2018
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular orientation and conformation of archaeal membrane lipid PGP-Me by solid-state NMR and its interaction with bacteriorhodopsin2018

    • Author(s)
      Yamagami Masaki、Tsuchikawa Hiroshi、Cui Jin、Kawatake Satoshi、Sato Fuminori、Umegawa Yuichi、Murata Michio、Seo Sangjar、Shinoda Wataru
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 固体NMRを用いたメチル分枝フィタノイル脂肪酸を有するリン脂質の特異な膜物性の解析2018

    • Author(s)
      斧拓冶、土川博史、山上正輝、梅川雄一、村田道雄
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 重水素化スフィンゴミエリンのアシル鎖の深度依存的な配座の赤外分光解析とコレステロールの添加効果2018

    • Author(s)
      佐伯直香、土川博史、門司真美、梅川雄一、村田道雄、J. Peter Slotte
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] スフィンゴミエリン鏡像体を用いたラフトモデル膜中での脂質間相互作用解析2017

    • Author(s)
      矢野陽、花島慎弥、土川博史、村田道雄、木下祥尚、松森信明、J. Peter Slotte
    • Organizer
      日本ケミカルバイオロジー学会第12回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 同位体標識スフィンゴミエリンの緩和時間測定に基づく脂質分子運動の新しい評価方法の開発2017

    • Author(s)
      木下雄哉、梅川雄一、土川博史、村田道雄
    • Organizer
      第7回CSJ化学フェスタ
    • Related Report
      2017 Annual Research Report
  • [Presentation] コレステロール側鎖の重水素標識体の合成とLo/Ld相による運動性変化と分配比の解明2017

    • Author(s)
      井端悠貴、花島慎弥、安田智一、村田道雄
    • Organizer
      第7回CSJ化学フェスタ
    • Related Report
      2017 Annual Research Report
  • [Presentation] Cholesterol interaction and membrane-permeabilizing properties of OSW-1 examined by solid-state NMR and fluorescence microscopy2017

    • Author(s)
      Malabed Raymond、Hanashima Shinya、Murata Michio、Sakurai Kaori
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Solid state NMR study for the molecular orientation of amphotericin B in membrane2017

    • Author(s)
      Yamamoto Tomoya、Umegawa Yuichi、Yamagami Masaki、Hanashima Shinya、Tsuchikawa Hiroshi、Matsumori Nobuaki、Murata Michio
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 固体NMRを用いた脂質二重膜におけるスフィンゴミエリン頭部の動的な配座解析2017

    • Author(s)
      村上量弘、花島慎弥、梅川雄一、土川 史、村田道雄、松森信明、J. Peter Slotte
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Enantioselective total synthesis of purple membrane lipid PGP-Me toward elucidation of protein- lipid interactions2017

    • Author(s)
      Yamagami Masaki、Cui Jin、Tsuchikawa Hiroshi、Umegawa Yuichi、Hanashima Shinya、Murata Michio
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] Membrane behavior and sterol interactions of sphigomyelin antipode2017

    • Author(s)
      Yano Yo、Hanashima Shinya、Yasuda Tomokazu、Tuchikawa Hiroshi、Murata Michio、Kinoshita Masanao、Matumori Nobuaki、Suzuki Kenichi、Slotte J. Peter
    • Organizer
      日本化学会第97春季年会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 2H,19F NMRを用いた脂質膜中におけるアンフォテリシンBの配向解析2016

    • Author(s)
      山本智也、梅川雄一、山上正輝、花島慎弥、土川博史、松森信明、村田道雄
    • Organizer
      第55回NMR討論会
    • Related Report
      2016 Annual Research Report
  • [Presentation] 重水素固体NMR測定における水和二重膜の温度測定のための金属錯体の合成と評価2016

    • Author(s)
      下西剛史、梅川雄一、門司真美、花島慎弥、土川博史、村田道雄
    • Organizer
      第38回生体膜と薬物の相互作用シンポジウム
    • Related Report
      2016 Annual Research Report
  • [Presentation] 脂肪酸結合タンパク質FABPの基質認識機構の解明を目指した環状脂肪酸の合成と親和性評価2016

    • Author(s)
      永安麻優、土川博史、杉山成、松岡茂、原利明、村田道雄
    • Organizer
      CSJ化学フェスタ2016
    • Related Report
      2016 Annual Research Report
  • [Remarks] 大阪大学大学院理学研究科生体分子化学研究室(村田研究室)

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/murata/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 村田研究室HP

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/murata/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 村田研究室・研究紹介(日本語)

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/murata/research.html

    • Related Report
      2018 Annual Research Report
  • [Remarks] Research Projects, Murata Laboratory

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/murata/english/research.html

    • Related Report
      2018 Annual Research Report
  • [Remarks]

    • URL

      http://www.chem.sci.osaka-u.ac.jp/lab/murata/research.html

    • Related Report
      2016 Annual Research Report
  • [Funded Workshop] 生体モデル膜国際セミナー ―化学の目から脂質二重膜と生体膜の学理と新機能を探る―2018

    • Related Report
      2018 Annual Research Report
  • [Funded Workshop] 生体モデル膜国際セミナー ―化学の目から脂質二重膜と生体膜の学理と新機能を探る―2017

    • Related Report
      2017 Annual Research Report

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Published: 2016-06-01   Modified: 2023-10-16  

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