Identification of the components of heat-responsible novel nuclear body containing MALAT1, a long noncoding RNA

Research Project

Project/Area Number	16H06743
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Biological pharmacy
Research Institution	The University of Tokyo
Principal Investigator	Onoguchi Rena 東京大学, アイソトープ総合センター, 特任助教 (30780697)
Project Period (FY)	2016-08-26 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000) Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	ノンコーディングRNA / 核内構造体 / 熱ストレス応答 / ストレス応答 / 長鎖ノンコーディングRNA
Outline of Final Research Achievements	Previously I found that localization of MALAT1 long noncoding RNA was altered and formed novel nuclear bodies in heat shock condition. In this project, I tried to identify the MALAT1-binding proteins as components of heat-inducible MALAT1 containing nuclear bodies. I initially fixed the experimental condition of affinity purification of MALAT1 RNA: proteins complexes. I identified the 70 kinds of proteins as MALAT1-binding proteins in heat shock condition. Because MALAT1-binding proteins contain the transcription factors and the splicing factors, the heat-inducible MALAT1-containing nuclear bodies may regulate transcription or splicing in heat shock response.

Report

(3 results)