Identification of the components of heat-responsible novel nuclear body containing MALAT1, a long noncoding RNA
| Project/Area Number |
16H06743
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Onoguchi Rena 東京大学, アイソトープ総合センター, 特任助教 (30780697)
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ノンコーディングRNA / 核内構造体 / 熱ストレス応答 / ストレス応答 / 長鎖ノンコーディングRNA |
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| Outline of Final Research Achievements |
Previously I found that localization of MALAT1 long noncoding RNA was altered and formed novel nuclear bodies in heat shock condition. In this project, I tried to identify the MALAT1-binding proteins as components of heat-inducible MALAT1 containing nuclear bodies. I initially fixed the experimental condition of affinity purification of MALAT1 RNA: proteins complexes. I identified the 70 kinds of proteins as MALAT1-binding proteins in heat shock condition. Because MALAT1-binding proteins contain the transcription factors and the splicing factors, the heat-inducible MALAT1-containing nuclear bodies may regulate transcription or splicing in heat shock response.
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Report
(3 results)
Research Products
(4 results)
