| Project/Area Number |
16H06769
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
General internal medicine(including psychosomatic medicine)
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 多発性筋炎 / ヒトiPS細胞 / 筋分化 / MHCクラスI / 多発性筋炎/皮膚筋炎 / サイトカイン |
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| Outline of Final Research Achievements |
MHC class I are more expressed on muscle fibers of polymyositis patients (PM Pts) than those of healthy donors (HDs). We considered to evaluate the differences of MHC class I expression and genetic properties between PM Pts and HDs by differentiating myocytes from human iPS cells (hiPSCs). If molecules that induce overexpression of MHC class I on PM muscles are identified, they can be targets for the treatment of PM.
MyoD-hiPSC bulks, which are composed of multiple MyoD-hiPSC clones, were established by transfecting MyoD, a myogenic transcription factor, into PM Pt or HD derived-hiPSC clones. We established also a differential condition for MyoD-hiPS bulks to differentiate into myocytes. Three MyoD-hiPSC bulks from the same donor differentiated similarly into myocytes. However, protein production differed between the three MyoD-hiPSC bulks. We concluded that it is difficult to evaluate the differences of MHC class I expression of PM Pts and HDs with myocytes derived from hiPSCs.
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