Influences of microglial lysosome dysfunction on the pathogenesis of autism spectrum disorders

Research Project

Project/Area Number	16H06829
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Psychiatric science
Research Institution	Kanazawa University
Principal Investigator	Zhu Hong 金沢大学, 子どものこころの発達研究センター, 博士研究員 (90778614)
Project Period (FY)	2016-08-26 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000) Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	自閉スペクトラム症 / ミクログリア
Outline of Final Research Achievements	To clarify whether lysosomal dysfunction in microglia was involved in the pathogenesis of developmental disorders, we tried to established experimental models by injecting pregnant rats with poly (I:C) (polyinosinic-polycytidylic acid). By immunohistochemical staining of embryonic brains, we find out changes in lysosome-related proteins as follows: (1) Heat shock protein 70 expression was augmented in the vascular wall. (2) Lysosomal stress-related Gpnmb (glycoprotein non-metastatic melanoma B) expression was enhanced in glial cells. These data suggest that lysosomal membranes become destabilized during the pathogenesis of developmental disorders.

Report

(3 results)