The role of long non-coding RNA in the development and suppressive function of regulatory T cells

• Project/Area Number: 16H06947
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Osaka University
• Principal Investigator: Ichiyama Kenji 大阪大学, 免疫学フロンティア研究センター, 特任助教(常勤) (60777960)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 制御性T細胞 / Foxp3 / 長鎖非翻訳RNA

Outline of Final Research Achievements

In order to identify the novel long non-coding RNAs (LncRNAs) which are important in the immunosuppressive function of regulatory T cell (Treg), LncRNAs, which are highly expressed in Treg and bound to Foxp3, were comprehensively analyzed by next generation sequencer. As a result, 37 novel LncRNAs were identified as candidates for Treg-specific functional LncRNA. Furthermore, we found a LncRNA which control the expression of Treg signature gene by the knock-down experiment using antisense oligonucleotides. In addition, we also identified the transcription factor X as a co-factor of Satb1, which is an essential factor for the development of Treg in the thymus, and found that RNA is necessary for the interaction between Satb1 and transcription factor X.

Research Products

• [Journal Article] Generation of RORγt+ Antigen-Specific T Regulatory 17 Cells from Foxp3+ Precursors in Autoimmunity. (2017)
  • Author(s): Kim BS, Lu H, Ichiyama K, Chen X, Zhang YB, Mistry NA, Tanaka K, Lee YH, Nurieva R, Zhang L, Yang X, Chung Y, Jin W, Chang SH, Dong C.
  • Journal Title: Cell Reports Volume: 21 Issue: 1 Pages: 195-207
  • DOI: 10.1016/j.celrep.2017.09.021
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Identification and functional elucidation of novel regulators in the development of IL-17-producing helper T cells (2017)
  • Author(s): 市山健司
  • Organizer: 第４６回日本免疫学会学術集会
• [Book] 周産期医学 (2017)
  • Author(s): 市山健司、坂口志文
  • Total Pages: 6
  • Publisher: 東京医学社