Functions of PDGFRa positive mesenchymal stem cells during the treatment of epidermolysis bullosa

• Project/Area Number: 16H06951
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dermatology
• Research Institution: Osaka University
• Principal Investigator: Shimbo Takashi 大阪大学, 医学系研究科, 特任助教(常勤) (70780609)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 表皮水疱症 / 間葉系幹細胞 / 網羅的遺伝子発現解析 / 単一細胞 / Pdgfra / 再生医学 / 皮膚科学 / 遺伝病 / 遺伝子発現解析 / 単一細胞遺伝子発現網羅的解析 / 間葉系細胞

Outline of Final Research Achievements

Epidermolysis bullosa (EB) is an intractable skin genetic disorder. Previously, we found that bone marrow Pdgfra positive cells have important roles in effective tissue regeneration in EB. Here, we performed whole transcriptome analysis on bone marrow Pdgfra positive cells at a single cell level and clarified that the cells contained multiple functionally distinct cell populations. Our data will serve as a foundation for identification of the complexed tissue regeneration mechanism in EB.

Research Products

• [Presentation] 網羅的遺伝子発現解析による末梢循環PDGFRα陽性間葉系幹細胞の性状解析 (2018)
  • Author(s): 新保敬史、佐々木英嗣、北山智美、西田真実、山崎翔、大内雄矢、山本竜馬、Edward Wijaya、菊池康、金田安史、玉井克人
  • Organizer: 第17回日本再生医療学会総会
• [Presentation] Characterization of circulating stem/progenitor cells during fatal skin injury regeneration (2017)
  • Author(s): Takashi Shimbo, Eiji Sasaki, Tomomi Kitayama, Mami Nishida, Sho Yamazaki, Yuya Ouchi, Sachiko Yamaoka,Yasushi Kikuchi, Yasufumi Kaneda, Katsuto Tamai
  • Organizer: 日本研究皮膚科学会
• [Presentation] Comprehensive analysis on HMGB1-induced circulating cells during cutaneous tissue regeneration (2017)
  • Author(s): Takashi Shimbo, Mami Nishida,Tomomi Kitayama,Ryoma Yamamoto,Eiji Takaki,Yasushi Kikuchi,Yasufumi Kaneda, and Katsuto Tamai
  • Organizer: The 47th European Society for Dermatology Research
  • Int'l Joint Research
• [Presentation] HMGB1 ADMINISTRATION AMELIORATES CUTANEOUS AND NON-CUTANEOUS MANIFESTATIONS IN THE MOUSE MODEL FOR AN INHERITED CONNECTIVE TISSUE DISEASE, DYSTROPHIC EPIDERMOLYSIS BULLOSA (2017)
  • Author(s): Takashi Shimbo, Sho Yamazaki, Xin Wang, Yasushi Kikuchi, Leena Bruckner-Tuderman, Yasufumi Kaneda, Katsuto Tamai
  • Organizer: The International Society for Stem Cell Research
  • Int'l Joint Research
• [Presentation] Systemic HMGB1 administration ameliorates cutaneous and non-cutaneous manifestations in a dystrophic epidermolysis bullosa model mouse (2017)
  • Author(s): Takashi Shimbo, Sho Yamazaki, Xin Wang, Yasushi Kikuchi, Leena Bruckner-Tuderman, Yasufumi Kaneda,Katsuto Tamai
  • Organizer: Society for Investigative Dermatology
  • Int'l Joint Research
• [Presentation] 末梢循環間葉系幹細胞による組織再生制御 (2017)
  • Author(s): 新保 敬史
  • Organizer: 日本炎症再生医学会
  • Invited
• [Presentation] Systemic delivery of HMGB1 ameliorates cutaneous and non-cutaneous manifestations in a dystrophic epidermolysis bullosa model mouse (2017)
  • Author(s): Takashi Shimbo, Sho Yamazaki, Xin Wang, Yasushi Kikuchi, Leena Bruckner-Tuderman, Yasufumi Kaneda, Katsuto Tamai
  • Organizer: 日本遺伝子細胞治療学会