A novel Wnt signaling pathway in ameloblast differentiation during tooth development

• Project/Area Number: 16H07065
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Kyushu University
• Principal Investigator: Miyazaki Kanako 九州大学, 大学病院, 助教 (30778840)
• Research Collaborator: YOSHIZAKI Keigo 九州大学, 歯学研究科(研究院), 助教 (10507982) ; ARAI Chieko 九州大学, 大学病院, 研究員 (60802288)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯学 / 細胞・組織 / 発生・分化 / シグナル伝達 / 歯

Outline of Final Research Achievements

Wnt signaling pathway plays important roles for tooth development, especially during the tooth morphogenesis. By using comprehensive analysis, we focused on Plakophilin 1 (PKP1) which is highly expressed in tooth. PKP1 is known as an adhesion-related molecule of desmosome and a member of the armadillo repeat domain, which is related to Wnt signaling pathway. In this study, we analyzed the intracellular localization of PKP1 during tooth development and suggested that PKP1 is involved in early tooth morphogenesis as an Wnt signal regulator, and regulates differentiation of ameloblast via the cell adhesion molecules.

Research Products

• [Journal Article] Nephronectin plays critical roles in Sox2 expression and proliferation in dental epithelial stem cells via EGF-like repeat domains. (2017)
  • Author(s): Arai C, Yoshizaki K, Miyazaki K, Saito K, Yamada A, Han X, Funada K, Fukumoto E, Haruyama N, Iwamoto T, Takahashi I, Fukumoto S.
  • Journal Title: Sci Rep. Volume: 27 Issue: 1 Pages: 45181-45181
  • DOI: 10.1038/srep45181
  • NAID: 120006534986
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] The roles of miR875-5p as a specific marker during early tooth development (2018)
  • Author(s): Keita Funada, Keigo Yosizaki, Xue Han, Kanako Miyazaki, Chieko Arai, Tomomi Yuta, Ichiro Takahashi
  • Organizer: Kyudai Oral Bioscience 2018 (KOB2018)
  • Int'l Joint Research
• [Presentation] PKP1 regulates ameloblast differentiation via the cell adhesion (2017)
  • Author(s): Kanako Miyazaki, Keigo Yoshizaki, Chieko Arai, Xue Han, Keita Funada, Naoto Haruyama, Satoshi Fukumoto, Ichiro Takahashi
  • Organizer: IADR
  • Place of Presentation: San Francisco, Calif., USA
  • Year and Date: 2017-03-22
  • Int'l Joint Research
• [Presentation] 基底膜分子NephronectinはRGD領域を介してエナメル芽細胞の分化制御に関与する (2017)
  • Author(s): 湯田 智美, 吉崎 恵悟, 新井 智映子, 宮崎 佳奈子, 韓 雪, 鮒田 啓太, 野口 健志, 高橋 一郎
  • Organizer: 第76回日本矯正歯科学会学術大会
• [Presentation] 密着接合因子ZO-1による歯原性上皮細胞の増殖および分化に与える影響 (2017)
  • Author(s): 鮒田 啓太, 吉崎 恵悟, 宮崎 佳奈子, 韓 雪, 新井 智映子, 湯田 智美, 高橋 一郎
  • Organizer: 第76回日本矯正歯科学会学術大会
• [Presentation] 歯に特異的に発現するmiR875-5pの同定と発現パターン解析 (2017)
  • Author(s): 吉崎 恵悟, 鮒田 啓太, 宮崎 佳奈子, 新井 智映子, 韓 雪, 湯田 智美, 高橋 一郎.
  • Organizer: 第76回日本矯正歯科学会学術大会
• [Presentation] The role of Nkx2-3 homeobox transcription factor in tooth development (2017)
  • Author(s): Han Xue, Yoshizaki Keigo, Arai Chieko, Miyazaki Kanako, Funada Keita, Yuta Tomomi, Takahashi Ichiro
  • Organizer: 第76回日本矯正歯科学会学術大会
• [Presentation] アレンドロネートの周期的静脈投与を受けている成長期骨形成不全症患者3症例の顎顔面形態の特徴 (2017)
  • Author(s): 宮崎 佳奈子、春山 直人、野口 健志、野村 俊介、吉崎 恵悟、高橋 一郎
  • Organizer: 第76回日本矯正歯科学会学術大会