Roles of a lysosomal transcription factor in osteoblast differentiation and inflammatory diseases

Research Project

Project/Area Number	16H07077
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Orthodontics/Pediatric dentistry
Research Institution	Nagasaki University
Principal Investigator	YONESHIMA Erika 長崎大学, 医歯薬学総合研究科(歯学系), 客員研究員 (20779566)
Project Period (FY)	2016-08-26 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000) Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	骨系細胞分化 / リソソーム転写因子 / TFEB / リソソーム / 骨芽細胞 / 炎症性疾患 / 脂肪細胞
Outline of Final Research Achievements	Since osteoblasts actively synthesize bone matrix, the function of lysosome, which is a degradation system, has hardly been analyzed. Therefore, in this study, the effects of TFEB expression change on osteoblast, adipocyte and chondrocyte differentiation was analyzed. Knock down of TFEB suppressed osteoblast differentiation, whereas overexpression of TFEB promoted it. We also found that ATF4 and CHOP involved in the endoplasmic reticulum stress response undergo the negative control of the differentiation. From these findings, TFEB is considered to be a necessary factor for alleviating endoplasmic reticulum stress caused by osteoblast differentiation.