The relationship between CD163 macrophages and lipid metabolism

• Project/Area Number: 16H07079
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Experimental pathology
• Research Institution: Kumamoto University
• Principal Investigator: Saito Yoichi 熊本大学, 大学院生命科学研究部(医), 研究員 (20783567)
• Research Collaborator: FIJIWARA Yukio
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: マクロファージ / CD163 / 肥満 / 脂質代謝 / 慢性炎症 / 組織在住マクロファージ / 単球由来マクロファージ / 卵黄嚢マクロファージ / 病理学 / 脂質 / 糖尿病 / 細菌 / メタボリックシンドローム / 脂肪肝 / 腸内細菌

Outline of Final Research Achievements

CD163, Hb scavenger receptor, is expressed on macrophages. CD163 KO mice fed high fat diet showed resistance to obesity and had significantly lower body weight, liver weight, and visceral fat amount than WT mice, significantly. Intestinal bacterial flora of WT mice had also changed.
In the liver and epididymis of obese WT mice, CD163 positive cells were significantly reduced due to CD163 negative monocyte - derived macrophages infiltrating with tissue inflammation. Interestingly, monocyte-derived macrophages have never been able to express CD163.
These results suggested that CD163 plays an important role in early phase of obesity. In addition, it was suggested that CD163 may be a marker of tissue-resident macrophages.

Research Products

• [Journal Article] Natural compounds that regulate lymph node sinus macrophages: Inducing an anti-tumor effect by regulating macrophage activation. (2018)
  • Author(s): Fujiwara Y, Saito Y, Shiota T, Cheng P, Ikeda T, Ohnishi K, Takeya M, Komohara Y.
  • Journal Title: Journal of clinical and experimental hematopathology Volume: 58 Pages: 17-23
  • NAID: 130006512988
  • Peer Reviewed / Open Access
• [Journal Article] Tumour-infiltrating inflammatory and immune cells in patients with extrahepatic cholangiocarcinoma. (2018)
  • Author(s): Kitano Y, Okabe H, Yamashita YI, Nakagawa S, Saito Y, Umezaki N, Tsukamoto M, Yamao T, Yamamura K, Arima K, Kaida T, Miyata T, Mima K, Imai K, Hashimoto D, Komohara Y, Chikamoto A, Ishiko T, Baba H.
  • Journal Title: British Journal of Cancer Volume: 118 Pages: 171-180
  • Peer Reviewed / Open Access
• [Journal Article] The cell-cell interaction between tumor-associated macrophages and small cell lung cancer cells is involved in tumor progression via STAT3 activation. (2017)
  • Author(s): Iriki T, Ohnishi K, Fujiwara Y, Horlad H, Saito Y, Pan C, Ikeda K, Mori T, Suzuki M, Ichiyasu H, Kohrogi H, Takeya M, Komohara Y.
  • Journal Title: Lung Cancer Volume: 106 Pages: 22-32
  • Peer Reviewed / Open Access
• [Presentation] マウスにおけるCD163を使ったマクロファージの起源解析の可能性 (2017)
  • Author(s): 西東洋一
  • Organizer: 第106回 日本病理学会総会
• [Presentation] マウスの単球由来マクロファージと組織在住マクロファージにおけるCD163発現の違い (2016)
  • Author(s): 西東 洋一
  • Organizer: 第62回日本病理学会秋期特別総会
  • Place of Presentation: 金沢市文化ホール
  • Year and Date: 2016-11-10