| Project/Area Number |
16H07342
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Developmental biology
|
|---|
| Research Institution | Ryukoku University |
|---|
Principal Investigator |
Kashima Makoto 龍谷大学, 龍谷大学 食と農の総合研究所, 博士研究員 (10780562)
|
|---|
| Research Collaborator |
MIYATA Atsumi 京都大学, 大学院・理学研究科, 研修員
|
|---|
| Project Period (FY) |
2016-08-26 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | プラナリア / 全能性幹細胞 / PIWI / piRNA / 幹細胞 / 免疫沈降 / RNA-seq |
|---|
| Outline of Final Research Achievements |
PIWI family proteins (PiwiB and PiwiC) are known to be important for the regulation of adult pluripotent stem cells in planarian. The mechanisms of the regulation by them remains elusive. Our previous researches revealed that PiwiB repress transposons and several functional protein coding genes, and that PiwiC and an antisense RNA of a transposon is involved in the repression of a transposon by PiwiB.
In this research, we got a results suggeting that PiwiC is involved in the production of PiwiB-interacting piRNAs from antisense RNA. Furthermore, I conducted RNA-Seq to indentufy PiwiC-dependent target genes of PiwiB.
|
