| Project/Area Number |
16H07448
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Genome biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
JIN JIANSHI (金 堅石) 国立研究開発法人理化学研究所, 生命システム研究センター, 特別研究員 (80783018)
|
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| Project Period (FY) |
2016-08-26 – 2018-03-31
|
| Project Status |
Discontinued (Fiscal Year 2017)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | T cell receptor / Antigen / High-throughput / Method / Recognizing / Identification method |
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| Outline of Annual Research Achievements |
In these months, I have cultured the T cells which express a specific T cell receptor, and the APCs (Antigen presenting cell) which present a specific antigen for the T cell. And also I have cultured another APC which presents a wrong paired antigen for that T cell as a negative control.
I have tried to identify the recognized T cell-APC pair in the mixture of T cells and APCs using a microscope. Using imaging, I have successfully found a pair of T cell interacted with its specific recognized APC. I also have tried to extract the T-cell-APC pairs from the solution.
For the high-throughput scanning, I have checked the scanning rate of the current microscope, it can scan more than 10^5 cells per 5 mins.
Until my cancellation of this grant, the other steps of my research plan are still ongoing.
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| Research Progress Status |
29年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
29年度が最終年度であるため、記入しない。
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