| Project/Area Number |
16H07482
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Okazaki Research Facilities, National Institutes of Natural Sciences |
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Principal Investigator |
Nozaki Mamoru 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 研究員 (50709631)
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | DMSO高感受性変異体 / 器官形成 / 不定根形成 / 細胞増殖制御 / DMSO高感受性 / 器官新形成 / 植物 / 形態形成 |
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| Outline of Final Research Achievements |
In this research, we isolated novel conditional mutants using enhanced dimethyl sulfoxide (DMSO) sensitivity instead of temperature sensitivity, and attempted to study the molecular mechanisms of plant organogenesis. By screening with adventitious root formation from hypocotyl explants as an index phenotype, we could isolate over 10 lines of DMSO sensitive mutants. The mutants showed several phenotypes related to cell proliferation under DMSO-containing tissue culture conditions. These phenotypes did not match to the phenotypes that were shared by the temperature sensitive mutants, suggesting that DMSO sensitive mutation might utilize as other type of conditional mutation for the study of plant organ development.
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