Analysis of myocardial viability by using Raman spectroscopy

• Project/Area Number: 16K01366
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Harada Yoshinori 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (10381956)
• Co-Investigator(Kenkyū-buntansha): 熊本 康昭 京都府立医科大学, 医学(系)研究科(研究院), 助教 (30611727)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: ラマン分光法 / 虚血心 / 生体譲歩う・計測

Outline of Final Research Achievements

In this study, we were sought to develop a basic technology that can analyze myocardial viability without staining using Raman spectroscopy. Raman microspectroscopy was used to search for Raman spectral features of ischemic heart diseases in hyperacute and chronic phases. Analyses of Langendorff-perfused rat hearts and old human myocardial infarcts revealed that Raman spectra became altered clearly in the hyperacute and chronic ischemic hearts. We expect that Raman spectroscopy will become discriminating of myocardial viability in vivo in the future.

Academic Significance and Societal Importance of the Research Achievements

通常、化学固定された組織で解析可能な心臓組織の観察が、ラマン分光法を用いることにより、生きたままの状態で解析できることが示された。ラマン分光法は染色の必要がないため、本法を術中観察へ応用すれば、心筋バイアビリティ診断に将来展開できる可能性がある。したがって、本手法は観血的に組織を採取する生検診断を患者に強いることなく、無侵襲で心臓組織診断を行うことにも発展しうる。

Research Products

• [Journal Article] ラマン分光法による生体組織・細胞解析とその可能性 (2019)
  • Author(s): 橋本 剛佑，原田 義規，熊本 康昭，高松 哲郎，田中 秀央
  • Journal Title: レーザー研究第47巻2号 Volume: 47 Pages: 99-102
  • NAID: 130008074027
  • Peer Reviewed
• [Journal Article] Label-free Molecular Imaging and Analysis by Raman Spectroscopy (2018)
  • Author(s): Kumamoto Y, Harada Y, Takamatsu T, Tanaka H
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 51 Issue: 3 Pages: 101-110
  • DOI: 10.1267/ahc.18019
  • NAID: 130007411560
  • ISSN: 0044-5991, 1347-5800
  • Year and Date: 2018-06-26
  • Peer Reviewed / Open Access
• [Journal Article] Label-free evaluation of myocardial infarct in surgically excised ventricular myocardium by Raman spectroscopy (2018)
  • Author(s): Yamamoto T, Minamikawa T, Harada Y, Yamaoka Y, Tanaka H, Yaku H, Takamatsu T
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 14671-14671
  • DOI: 10.1038/s41598-018-33025-6
  • NAID: 120006875669
  • Peer Reviewed / Open Access
• [Presentation] ラマン顕微鏡による生体組織解析 (2018)
  • Author(s): 原田義規、高松哲郎、田中秀央
  • Organizer: レーザー学会学術講演会第３８回年次大会シンポジウム
• [Presentation] 心筋梗塞巣のラマン分光分析 (2017)
  • Author(s): 原田義規、高松哲郎、田中秀央
  • Organizer: 第15回 医用分光学研究会
  • Invited
• [Presentation] Analysis of living cells and tissuesby label-free microscopy (2017)
  • Author(s): Yoshinori Harada, Tetsuro Takamatsu, Hideo Tanaka
  • Organizer: 第12回中日組織細胞化学合同セミナー
  • Int'l Joint Research