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Elucidation of octanoylated ghrelin secretion mechanism targeted towards regulating appetite and obesity

Research Project

Project/Area Number 16K01848
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied health science
Research InstitutionNagasaki International University

Principal Investigator

KARIYAZONO Hiroko  長崎国際大学, 薬学部, 教授 (20437958)

Co-Investigator(Kenkyū-buntansha) 大磯 茂  長崎国際大学, 薬学部, 准教授 (40513106)
Research Collaborator NAKAJIMA kensuke  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsオクタノイルグレリン / ドコサヘキサエン酸 / オレアノール酸 / AGS-GHRL8細胞 / 脂肪酸 / グレリン / 肥満
Outline of Final Research Achievements

Octanoylated ghrelin is a peptide hormone with an appetite stimulating effect. We found that docosahexaenoic acid (DHA) and oleanolic acid suppressed octanoylated ghrelin secretion from AGS-GHRL8 cells established by transfecting ghrelin cDNA into human gastric carcinoma AGS cells. We attempted to determine the suppressive mechanism of octanoylated ghrelin secretion by these compounds. It was considered that the suppressive effects of both the compounds were neither caused by the downregulation of ghrelin-O-acyltransferase expression, or through the activation of G-protein coupled receptor 120. Examination using the free fatty acid assay kit indicated the following mechanism: acyl-CoA products formed between the carboxyl group of DHA or oleanolic acid and coenzyme A may cause inhibition of octanoyl-CoA generation essential for ghrelin octanoylation, resulting in suppressive secretion of octanoylated ghrelin. These findings could contribute to regulation of appetite and obesity.

Academic Significance and Societal Importance of the Research Achievements

脂質異常症、糖尿病、高血圧症等の生活習慣病から虚血性心疾患、脳卒中等の動脈硬化性疾患にも発展しかねないメタボリックシンドロームならびに肥満の克服が医療費削減の側面からも重要視されている。摂食亢進活性を示すオクタノイルグレリンの産生・分泌抑制物質としてのドコサヘキサエン酸およびオレアノール酸の発見ならびにその抑制機序の解明は、新規機能性食品の開発等、肥満対策につながる成果として社会的意義がある。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (15 results)

All 2019 2018 2017 2016

All Journal Article (6 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Decreased Plasma Octanoylated Ghrelin Levels in Mice by Oleanolic Acid2019

    • Author(s)
      Nakajima K, Maeda N, Oiso S, Kariyazono H.
    • Journal Title

      Journal of Oleo Science

      Volume: 68 Issue: 1 Pages: 103-109

    • DOI

      10.5650/jos.ess18148

    • NAID

      130007541862

    • ISSN
      1345-8957, 1347-3352
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibitory Effect of (−)-Epigallocatechin-3-<i>O</i>-gallate on Octanoylated Ghrelin Levels <i>in Vitro</i> and <i>in Vivo</i>2018

    • Author(s)
      Nakajima K, Oiso S, Kariyazono H.
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 41 Issue: 4 Pages: 524-529

    • DOI

      10.1248/bpb.b17-00805

    • NAID

      130006602523

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Bioactive Triterpenes from the Root of Salvia miltiorrhiza Bunge.2017

    • Author(s)
      Tung NH, Nakajima K, Uto T, Hai NT, Long DD, Ohta T, Oiso S, Kariyazono H, Shoyama Y.
    • Journal Title

      Phytotherapy Research

      Volume: 31 Issue: 9 Pages: 1457-1460

    • DOI

      10.1002/ptr.5877

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Bioactivities of Eriobotrya japonica (Thunb.) Lindl. Leaf and Its Triterpenes2017

    • Author(s)
      Uto T, Tung NH, Nakajima K, Ohta T, Oiso S, Kariyazono H, Shoyama Y
    • Journal Title

      Journal of Pharmacognosy and Natural Products

      Volume: 印刷中 Issue: 01 Pages: 134-134

    • DOI

      10.4172/2472-0992.1000134

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] <b>Triterpenes suppress octanoylated ghrelin production in ghrelin-expressing human gastric carcinoma </b><b>cells </b>2016

    • Author(s)
      Nakajima K, Oiso S, Uto T, Morinaga O, Shoyama Y, Kariyazono H.
    • Journal Title

      Biomedical Research

      Volume: 37 Issue: 6 Pages: 343-349

    • DOI

      10.2220/biomedres.37.343

    • NAID

      130005290642

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] グレリン分泌抑制成分探索のための細胞評価系の構築と成分探索2016

    • Author(s)
      仮屋薗博子、大磯茂、中島健輔
    • Journal Title

      Foods & Food Ingredients Journal of Japan

      Volume: 221 Pages: 88-93

    • Related Report
      2016 Research-status Report
  • [Presentation] オレアノール酸のオクタノイルグレリン産生抑制効果とその機序2019

    • Author(s)
      中島 健輔、前田 成美、大磯 茂、仮屋薗 博子
    • Organizer
      日本薬学会第139年会(千葉市)
    • Related Report
      2018 Annual Research Report
  • [Presentation] オクタノイルグレリン産生・分泌に及ぼすドコサヘキサエン酸の影響とそのメカニズム2018

    • Author(s)
      濵田 祐成、永瀬 翔子、橋口 夏海、大磯 茂、中島 健輔、仮屋薗 博子
    • Organizer
      第35回日本薬学会九州支部大会(福岡)
    • Related Report
      2018 Annual Research Report
  • [Presentation] エイコサペンタエン酸およびドコサヘキサエン酸のオクタノイルグレリン産生・分泌抑制機序に関与する因子の解析2018

    • Author(s)
      橋口 夏海、永瀬 翔子、大磯 茂、中島 健輔、仮屋薗 博子
    • Organizer
      日本薬学会第138年会(金沢市)
    • Related Report
      2017 Research-status Report
  • [Presentation] In vitroおよびin vivoにおけるドコサヘキサエン酸のオクタノイルグレリン産生・分泌抑制効果2017

    • Author(s)
      大磯茂、永瀬翔子、中島健輔、仮屋薗博子
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report
  • [Presentation] エピガロカテキン没食子酸のオクタノイルグレリン産生抑制効果とその抑制機序の検討2017

    • Author(s)
      中島健輔、大磯茂、仮屋薗博子
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台国際センター(宮城県仙台市)
    • Year and Date
      2017-03-24
    • Related Report
      2016 Research-status Report
  • [Presentation] In vitroおよびin vivoにおけるオレアノール酸のオクタノイルグレリン産生・分泌抑制効果2017

    • Author(s)
      中島 健輔、大磯 茂、仮屋薗 博子
    • Organizer
      第34回日本薬学会九州支部大会(熊本市)
    • Related Report
      2017 Research-status Report
  • [Presentation] マウス血漿中オクタノイルグレリン濃度に及ぼすエピガロカテキン没食子酸およびグリチルレチン酸の影響2016

    • Author(s)
      中島健輔、大磯茂、宇都拓洋、森永紀、正山征洋、仮屋薗博子
    • Organizer
      第33回日本薬学会九州支部大会
    • Place of Presentation
      鹿児島大学(鹿児島県鹿児島市)
    • Year and Date
      2016-12-03
    • Related Report
      2016 Research-status Report
  • [Presentation] 食欲・肥満制御を目指した活性型グレリン分泌抑制物質探索におけるAGS-GHRL8細胞の有用性2016

    • Author(s)
      仮屋薗博子、大磯茂、野邉みゆき、山口祐平、岩崎集平、後藤夏美、関夕佳里、中島健輔
    • Organizer
      第26回日本医療薬学会年会
    • Place of Presentation
      国立京都国際会館(京都府京都市)
    • Year and Date
      2016-09-17
    • Related Report
      2016 Research-status Report
  • [Patent(Industrial Property Rights)] 食欲抑制剤2017

    • Inventor(s)
      仮屋薗博子、大磯茂、対馬忠広、田畑宏、三澤嘉久
    • Industrial Property Rights Holder
      仮屋薗博子、大磯茂、対馬忠広、田畑宏、三澤嘉久
    • Industrial Property Rights Type
      特許
    • Filing Date
      2017
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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