Development of a practical protein-chip using designed DNA-arrays

• Project/Area Number: 16K01910
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomolecular chemistry
• Research Institution: Hirosaki University
• Principal Investigator: Hagihara Masaki 弘前大学, 理工学研究科, 准教授 (40403000)
• Project Period (FY): 2016-10-21 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: グアニン四重鎖 / 分子センサー / プロテインチップ

Outline of Final Research Achievements

This research proposal aims to create a superior protein analysis tool (protein detection device) with a viewpoint of convenience, quickness, high inspection accuracy, reproducibility, low cost etc. at a level that can be used in a clinical purpose. Here, we showed that "nucleic acid structure library" , that is rich in guanine bases, can serve as a new class of protein recognition elements.

Academic Significance and Societal Importance of the Research Achievements

今回，有機合成化学的手法と，遺伝子工学手法を融合することにより，核酸の安定な高次構造として知られるグアニン四重鎖構造を指向した多様な DNA 型素子ライブラリーを構造や配列による偏りを極力排除した形で作製することに成功した.四重鎖構造体をライブ ラリー化する今回の研究成果は技術的新規性，優位性が保証されると考えている.

Research Products

• [Journal Article] Structures and Catalytic Activities of Complexes between Heme and All Parallel-Stranded Monomeric G-Quadruplex DNAs (2018)
  • Author(s): Yamamoto Yasuhiko、Araki Haruka、Shinomiya Ryosuke、Hayasaka Kosuke、Nakayama Yusaku、Ochi Kentaro、Shibata Tomokazu、Momotake Atsuya、Ohyama Takako、Hagihara Masaki、Hemmi Hikaru
  • Journal Title: Biochemistry Volume: 57 Issue: 41 Pages: 5938-5948
  • DOI: 10.1021/acs.biochem.8b00792
  • Peer Reviewed
• [Journal Article] A small molecule that represses translation of G-quadruplex-containing mRNA. (2016)
  • Author(s): Katsuda, Y., Sato, S., Asano, L., Morimura, Y., Furuta, T., Sugiyama, H., Hagihara, M., Uesugi, M.
  • Journal Title: J. Am. Chem. Soc. Volume: 138 Issue: 29 Pages: 9037-9040
  • DOI: 10.1021/jacs.6b04506
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 有機小分子によるDNA繰り返し配列短縮効果 (2018)
  • Author(s): 齋藤薫・堂野主税・中谷和彦・萩原正規
  • Organizer: 第12回バイオ関連化学シンポジウム
• [Presentation] 人工分子による核酸構造の制御 ーキャピラリー電気泳動法を利用した核酸構造のマッピング ー (2018)
  • Author(s): 萩原正規
  • Organizer: 第 25 回クロマトグラフィーシンポジウム(弘前)
  • Invited
• [Presentation] RNAグアニン四重鎖の構造と機能制御 (2017)
  • Author(s): 萩原正規
  • Organizer: 第二回先端ケミカルバイオロジー研究会
  • Invited
• [Presentation] ヘムと四重鎖 DNA の複合体の構造解析 (2017)
  • Author(s): 越智 健太郎・山本 泰彦・柴田 友和・萩原 正規・逸見 光
  • Organizer: 日本化学会第97春季年会（2017）
• [Presentation] Heme-DNA Complexes (2017)
  • Author(s): Yasuhiko Yamamoto, Tomokazu Shibata, Yuya Katahira, Kentaro Ochi, Yusaku Nakayama, Hulin Tai, Akari Watanabe, Tomomi Nakao, Sachiko Yanagisawa, Takashi Ogurad, Hikaru Hemmi, Masaki Hagihara, Akihiro Suzuki, and Saburo Neyah
  • Organizer: 14th International Symposium on Applied Bioinorganic Chemistry
  • Int'l Joint Research