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Novel preparation method for hybrid films of water-soluble proteins and inorganic nanosheets by the LB technique --- application of electrospray spreading

Research Project

Project/Area Number 16K05762
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional solid state chemistry
Research Institution防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工学群)

Principal Investigator

Umemura Yasushi  防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工学群), 応用科学群, 教授 (70531771)

Co-Investigator(Kenkyū-buntansha) 山岸 晧彦  東邦大学, 医学部, 非常勤研究生 (70001865)
宮内 良広  防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工学群), 応用科学群, 准教授 (70467124)
平原 将也  防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工学群), 応用科学群, 講師 (90609835)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsエレクトロスプレー法 / 水溶性タンパク / LB膜 / 粘土ナノシート / オクタデシルアンモニウムイオン / 噴射速度 / リゾチーム / オクタデシルアンモニウム / 有機/無機複合薄膜 / LB法 / エレクトロ・スプレー法 / 噴霧速度 / 両親媒性イオン / アルキルアンモニウムイオン / 薄膜形成メカニズム / 無機ナノシート / 水溶性両親媒性陽イオン / 水溶性両親媒性分子 / ラングミュア-ブロジェット(LB)法 / エレクトロ・スプレー(ES)法 / タンパク / 機能性超薄膜
Outline of Final Research Achievements

When a chloroform solution including an amphiphilic ion is dropped on an aqueous dispersion of an inorganic nanosheet, a hybrid film of the amphiphilic ion and the nanosheet is formed. In a conventional method, the solution is spread by hand using a microsyringe. In this work, the solution is spread as fine mists by an electrospraying method. One of the results is that properties of the hybrid films prepared by spraying an alkylammonium salt solution on a dilute clay dispersion is dependent on the infusion rate. As another result, lysozyme, a water-soluble protein, forms a stable hybrid film with the clay nanosheets. Some proteins and amino acids, however, do not form stable films by this method.

Academic Significance and Societal Importance of the Research Achievements

水に対してやや溶解する両親媒性イオンでも、無機ナノシート水分散液上に展開すると安定な単分子膜が形成されることは知られていた。本研究では両親媒性イオン溶液の展開にエレクトロスプレー法を初めて用いた結果、展開速度を定量的に評価することが可能となり、薄膜の性質が噴霧速度に大きく依存することが示された。マイクロシリンジを使って手で展開すると薄膜の性質に個人差が生じたが、今回の方法では個人差はない。すなわち、将来の産業化において、均一な品質の保持にも対応可能である。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (40 results)

All 2020 2019 2018 2017 2016

All Journal Article (12 results) (of which Peer Reviewed: 12 results,  Open Access: 6 results) Presentation (25 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (3 results)

  • [Journal Article] Real-Time Monitoring of Low Pressure Oxygen Molecules over Wide Temperature Range: Feasibility of Ultrathin Hybrid Films of Iridium(III) Complexes and Clay Nanosheets2020

    • Author(s)
      Sato Hisako、Takimoto Kazuyoshi、Kato Masaru、Nagaoka Shin-ichi、Tamura Kenji、Yamagishi Akihiko
    • Journal Title

      Bulletin of the Chemical Society of Japan

      Volume: 93 Issue: 2 Pages: 194-199

    • DOI

      10.1246/bcsj.20190277

    • NAID

      130007798346

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of electrospray spreading to a modified Langmuir-Blodgett technique for organo-clay hybrid film preparation2019

    • Author(s)
      Masanari Hirahara, Hiroki Kaida, Yoshihiro Miyauchi, Hiroki Goto, Akihiko Yamagishi, Yasushi Umemura
    • Journal Title

      Colloids and Surfaces A

      Volume: 580 Pages: 123714-123714

    • DOI

      10.1016/j.colsurfa.2019.123714

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple-peak resonance of optical second harmonic generation arising from band nesting in monolayer transition metal dichalcogenides TX2 on SiO2/Si(001) substrates (T = Mo, W; X = S, Se)2019

    • Author(s)
      Y.Kikuchi, Y. Miyauchi, R. Takaoka, T. Suzuki, M. Tanaka, and S. Ohno
    • Journal Title

      Physical Review B

      Volume: 100 Issue: 7

    • DOI

      10.1103/physrevb.100.075301

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Photoisomerization and thermal isomerization of ruthenium aqua complexes with chloro-substituted asymmetric bidentate ligands2019

    • Author(s)
      M. Hirahara, H. Goto, Rei. Yamamoto, M. Yagi, and Y. Umemura
    • Journal Title

      RSC Advances

      Volume: 9 Issue: 4 Pages: 2002-2010

    • DOI

      10.1039/c8ra08943d

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Observation by optical second harmonic generation of the mean tilt angle of cyanine dyes during compression with a phase transition in a Langmuir-Blodgett trough2019

    • Author(s)
      Y.Miyauchi, R. Yukutake, K. Tsuchida, Y. Umemura, A. Tsukamoto, and T. Suzuki
    • Journal Title

      Chemical Physics

      Volume: 517 Pages: 85-90

    • DOI

      10.1016/j.chemphys.2018.09.028

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Microscopic chiral pockets in a tris(chelated) iridium(iii) complex as sites for dynamic enantioselective quenching2018

    • Author(s)
      Takimoto Kazuyoshi、Tamura Kenji、Watanabe Yutaka、Yamagishi Akihiko、Sato Hisako
    • Journal Title

      New Journal of Chemistry

      Volume: 42 Issue: 7 Pages: 4818-4823

    • DOI

      10.1039/c7nj04688j

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chiral Tectonics: VCD and ECD Application for Epimerization of a Star-Burst Tetranuclear Complex with a Labile Central Core2018

    • Author(s)
      Sato Hisako、Sato Fumi、Yamagishi Akihiko
    • Journal Title

      Inorganics

      Volume: 6 Issue: 3 Pages: 70-70

    • DOI

      10.3390/inorganics6030070

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Stereoselective interactions as manifested by vibrational circular dichroism spectra: the interplay between chiral metal complexes co-adsorbed in a montmorillonite clay2018

    • Author(s)
      Sato Hisako、Takimoto Kazuyoshi、Mori Hirotoshi、Yamagishi Akihiko
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 20 Issue: 39 Pages: 25421-25427

    • DOI

      10.1039/c8cp04753g

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Solid state vibrational circular dichroism towards molecular recognition: chiral metal complexes intercalated in a clay mineral2018

    • Author(s)
      Sato Hisako、Tamura Kenji、Takimoto Kazuyoshi、Yamagishi Akihiko
    • Journal Title

      Physical Chemistry Chemical Physics

      Volume: 20 Issue: 5 Pages: 3141-3147

    • DOI

      10.1039/c7cp05114j

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hybrid film formation of a water-insoluble quaternary alkylammonium cation with clay-mineral-layers2017

    • Author(s)
      Hirahara Masanari、Umemura Yasushi
    • Journal Title

      Thin Solid Films

      Volume: 642 Pages: 377-383

    • DOI

      10.1016/j.tsf.2017.09.058

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mechanistic Insight into Reversible Core Structural Changes of Dinuclear μ-Hydroxoruthenium(II) Complexes with a 2,8-Di-2-pyridyl-1,9,10-anthyridine Backbone Prior to Water Oxidation Catalysis2017

    • Author(s)
      Hirahara Masanari、Nagai Sho、Takahashi Kosuke、Watabe Shunsuke、Sato Taisei、Saito Kenji、Yui Tatsuto、Umemura Yasushi、Yagi Masayuki
    • Journal Title

      Inorganic Chemistry

      Volume: 56 Issue: 17 Pages: 10235-10246

    • DOI

      10.1021/acs.inorgchem.7b00978

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Oxygen Sensing by the Hybrid Langmuir-Blodgett Films of Iridium(III) Complexes and Synthetic Saponite on the Basis of Energy Transfer2017

    • Author(s)
      Sato Hisako、Tamura Kenji、Yamagishi Akihiko
    • Journal Title

      Chemosensors

      Volume: 5 Issue: 4 Pages: 27-27

    • DOI

      10.3390/chemosensors5040027

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Characterization of LB monolayers composed of quaternary ammonium cations and clay nanosheets prepared by electro-spray spreading2020

    • Author(s)
      Yasushi UMEMURA, Masanari HIRAHARA
    • Organizer
      日本化学会第100春季年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Application of electrospray spreading to organo-clay hybrid film preparation by a modified Langmuir-Blodgett technique2019

    • Author(s)
      Yasushi Umemura, Masanari Hirahara, Yoshihiro Miyauchi, Hiroki Goto
    • Organizer
      Euroclay 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] In-situ SHG measurements in the monolayer during the compression process with a phase transition in a Langmuir-Blodgett trough2019

    • Author(s)
      Y. Miyauchi, K. Nakamura, Y. Umemura, A, Tsukamoto, and T. Suzuki
    • Organizer
      ALC '19
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アルキル鎖長の異なる4級アルキルアンモニウムイオンと粘土ナノシートとのエレクトロ・スプレー法による複合薄膜調製2019

    • Author(s)
      梅村泰史,平原将也
    • Organizer
      第63 回粘土科学討論会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 外部刺激応答性を示すルテニウムアクア錯体単分子膜の性質2019

    • Author(s)
      後藤寛紀, 平原将也, 梅村泰史
    • Organizer
      第9回CSJ化学フェスタ2019
    • Related Report
      2019 Annual Research Report
  • [Presentation] 外部刺激応答性を示すルテニウムアクア錯体単分子膜の性質2019

    • Author(s)
      後藤寛紀, 平原将也, 梅村泰史
    • Organizer
      日本化学会第99春季年会
    • Related Report
      2018 Research-status Report
  • [Presentation] エレクトロ・スプレー法を用いた両親媒性4級アンモニウムイオンと粘土ナノシートとの複合薄膜調製2018

    • Author(s)
      梅村 泰史, 平原 将也
    • Organizer
      第62回粘土科学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] エレクトロ・スプレー法を用いた有機/粘土複合LB膜の調製2018

    • Author(s)
      梅村泰史, 平原将也, 後藤寛紀, 宮内良広
    • Organizer
      第69回コロイドおよび界面化学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] 可視光により異性化するルテニウムアクア錯体を用いて調製された単分子膜の性質2018

    • Author(s)
      後藤寛紀, 平原将也, 梅村泰史
    • Organizer
      第8回CSJ化学フェスタ2018
    • Related Report
      2018 Research-status Report
  • [Presentation] 合成サポナイトに吸着したキラルイリジウム錯体の発光挙動2018

    • Author(s)
      瀧本和誉・渡邉裕・山岸晧彦・佐藤久子
    • Organizer
      日本化学会第98春季年会(2018)
    • Related Report
      2017 Research-status Report
  • [Presentation] Preparation of Alkylammonium Cation and Clay Nanosheet Ultrathin Hybrid Films by an Electrospray Method2017

    • Author(s)
      Yasushi Umemura, Hiroki Kaida, Masasnari Hirahara
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県・横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] パーフルオロ基をもつゲル化剤の単分子膜の性質2017

    • Author(s)
      山岸晧彦、矢島知子、田村堅志、佐藤久子
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県・横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] Fabrication of 3-Layer-Component Organo-Clay Hybrid Films with Reverse Deposition Orders by a Modified Langmuir-Schaefer Technique and their Pyroelectric Currents Measured by a Non-Contact Method2017

    • Author(s)
      Yasushi Umemura, Masanari Hirahara
    • Organizer
      XVI International Clay Conference
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 光異性化するRu(Ⅱ)錯体の粘土鉱物層間へのインターカレーション2017

    • Author(s)
      平原 将也, 辻本 真喜子, 石橋 和英, 今泉 仁志, 梅村 泰史
    • Organizer
      錯体化学会第67回討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] エレクトロ・スプレー法を利用して調製された有機/粘土複合LB膜の解析2017

    • Author(s)
      梅村 泰史, 平原 将也
    • Organizer
      第61回粘土科学討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] In-situ SHG measurements in the LB monolayer during the compression process2017

    • Author(s)
      2.Y. Miyauchi, R. Yukutake, K. Tsuchida, Y. Umemura and T. Suzuki
    • Organizer
      8th International Symposium on Surface Science
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Application of Vibrational Circular Dichroism to Chiral Iridium and Ruthenium Complexes2017

    • Author(s)
      佐藤久子,田村堅志,山岸晧彦
    • Organizer
      第67回錯体化学討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] 粘土鉱物へのイリジウム錯体吸着による発光キラルセンシグ2017

    • Author(s)
      山岸晧彦 ・瀧本和誉 ・田村堅志 ・渡邉裕 ・佐藤久子
    • Organizer
      第61回粘土科学討論会
    • Related Report
      2017 Research-status Report
  • [Presentation] 水面上に浮遊する粘土ナノシート上での長鎖アルキルアンモニウム塩の自己集積2016

    • Author(s)
      梅村泰史、平原将也
    • Organizer
      第60回粘土科学討論会
    • Place of Presentation
      九州大学病院キャンパス(福岡県・福岡市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Research-status Report
  • [Presentation] 粘土カラムを用いた光学異性体の分離技術:きっかけ、発展そして現状2016

    • Author(s)
      山岸晧彦
    • Organizer
      第60回粘土科学討論会
    • Place of Presentation
      九州大学病院キャンパス(福岡県・福岡市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] 合成サポナイトとハイブリッド化したイリジウム錯体膜による酸素センシング2016

    • Author(s)
      佐藤久子、田村堅志、渡辺裕、山岸晧彦
    • Organizer
      第60回粘土科学討論会
    • Place of Presentation
      九州大学病院キャンパス(福岡県・福岡市)
    • Year and Date
      2016-09-15
    • Related Report
      2016 Research-status Report
  • [Presentation] キラルなルテニウム錯体と酸化チタンナノシートとの複合薄膜における光電流挙動2016

    • Author(s)
      梅村泰史、平原将也
    • Organizer
      錯体化学会第66回討論会
    • Place of Presentation
      福岡大学七隈キャンパス(福岡県・福岡市)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
  • [Presentation] 発光性イリジウム(III)錯体とアミノ酸との相互作用2016

    • Author(s)
      山岸晧彦、佐藤久子,北澤孝史、佐藤二美
    • Organizer
      錯体化学会第66回討論会
    • Place of Presentation
      福岡大学七隈キャンパス(福岡県・福岡市)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
  • [Presentation] イリジウム錯体LB膜のエネルギー移動を利用した酸素センシング2016

    • Author(s)
      佐藤久子、瀧本和誉、渡辺裕、山岸晧彦
    • Organizer
      錯体化学会第66回討論会
    • Place of Presentation
      福岡大学七隈キャンパス(福岡県・福岡市)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
  • [Presentation] Visible-Light Induced Morphological Changes of Giant Vesicles by a Photoresponsive Ruthenium Aqua Complex2016

    • Author(s)
      Masanari Hirahara, Akira Tsukamoto, Hiroki Goto, Shigeru Tada, Masayuki Yagi, Yasushi Umemura
    • Organizer
      錯体化学会第66回討論会
    • Place of Presentation
      福岡大学七隈キャンパス(福岡県・福岡市)
    • Year and Date
      2016-09-10
    • Related Report
      2016 Research-status Report
  • [Book] "Preparation and application of clay mineral films" in "Developments in Clay Science", Vol. 9, Chap. 122018

    • Author(s)
      Y. Umemura
    • Total Pages
      20
    • Publisher
      Elsevier
    • Related Report
      2018 Research-status Report
  • [Book] Inorganic Nanosheets and Related Materilas: Fumdamentals and Applications of Two-Dimensional Systems; (Chapter 2) Clay Minerals as Natural Nanosheets2017

    • Author(s)
      Robert Schoonheydt, Yasushi Umemura
    • Total Pages
      21
    • Publisher
      Springer
    • ISBN
      9784431564942
    • Related Report
      2017 Research-status Report
  • [Book] Inorganic Nanosheets and Related Materilas: Fumdamentals and Applications of Two-Dimensional Systems; (Chapter 20) Chirality and its application2017

    • Author(s)
      Hisako Sato and Akihiko Yamagishi
    • Total Pages
      18
    • Publisher
      Springer
    • ISBN
      9784431564942
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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