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Novel Approach of Solid-State NMR to Reveal Molecular Mechanism of Supramolecular Structural Formation of Biomolecules Through Lipid Bilayers

Research Project

Project/Area Number 16K05858
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionInstitute for Molecular Science

Principal Investigator

Nishimura Katsuyuki  分子科学研究所, 物質分子科学研究領域, 准教授 (00334631)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords固体NMR / アミロイドβ / オリゴマー / 脂質膜 / 構造解析 / 生体分子 / 脂質 / アミロイド / 開発
Outline of Final Research Achievements

We have explored to develop effective techniques of solid-state NMR for structural characterizations of peripheral membrane proteins and peptides which express their functions based on their supramolecular structures conformed by associations among them and interactions with lipid bilayers. On the other hand, amyloid-β has been considered to play crucial roles in the onset and developments of Alzheimer's disease through the fomation of insoluble amyloid fibrils. In this study, we have also explored structural characterization of oligomeric state amyloid-β(1-40) induced on model neuronal lipid membranes based on solid-state NMR.

Academic Significance and Societal Importance of the Research Achievements

アルツハイマー病は、多くの国民に直接関わる重要な疾患であるが、その発症機構の解明、および完治可能な治療薬の開発には至っていない。当該疾患の発症に深く関与することが示唆されているアミロイドβが形成する不溶性アミロイド線維形成の分子機構解明は、同疾患治療および予防の実現に必要不可欠な研究課題である。本研究は、この解明に大きく寄与することが期待され、学術的、社会的にもその意義は大きいと考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (10 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (6 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (3 results)

  • [Int'l Joint Research] The City University of New York(米国)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Effective Synthesis and Their Copolymerization of Fluorine-Containing Phenanthrene with Hydroxyl Group2019

    • Author(s)
      S. Kataoka, H. Fukumoto, T. Kawasaki-Takasuka, T. Yamazaki, K. Nishimura, T. Agou, T. Kubota
    • Journal Title

      Journal Fluorine Chemistry

      Volume: 218 Pages: 84-89

    • DOI

      10.1016/j.jfluchem.2018.11.017

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative Analysis of Solid-State Homonuclear Correlation Spectra of Antiparallel beta-Sheet Alanine Tetramers2018

    • Author(s)
      A. Naito, K. Okushita, K. Nishimura, G. S. Boutis, A. Aoki, and T. Asakura
    • Journal Title

      J. Phys. Chem. B

      Volume: 122 Issue: 10 Pages: 2715-2724

    • DOI

      10.1021/acs.jpcb.7b11126

    • Related Report
      2018 Annual Research Report 2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Helix-in-Helix" Superstructure Formation through Encapsulation of Fullerene-Bound Helical Peptides within a Helical Poly(Methyl Methacrylate) Cavity2017

    • Author(s)
      N. Ousaka, F. Mamiya, Y. Iwata, Y. Nishimura, E. Yashima
    • Journal Title

      Angew. Chem., Int. Ed.

      Volume: 56 Issue: 3 Pages: 791-795

    • DOI

      10.1002/anie.201611349

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular and electron-spin structures of a ring-shaped mixed-valence polyoxovanadate (IV, V) studied by 11B and 23Na solid-state NMR spectroscopy and DFT calculations2016

    • Author(s)
      Takahiro Iijima, Toshihiro Yamase, Katsuyuki Nishimura
    • Journal Title

      Solid State Nuclear Magnetic Resonance

      Volume: 76-77 Pages: 15-23

    • DOI

      10.1016/j.ssnmr.2016.03.004

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Multiple-component covalent organic frameworks2016

    • Author(s)
      N. Huang, L. Zhai, D. E. Coupry, M. A. Addicoat, K. Okushita, K. Nishimura, T. Heine, D. Jiang
    • Journal Title

      Nat. Comm

      Volume: 7 Issue: 1

    • DOI

      10.1038/ncomms12325

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structure and Dynamics of Membrane-Bound Proteins2016

    • Author(s)
      K. Nishimura, M. Tanio, and S. Tuzi
    • Journal Title

      Modern Magnetic Resonance

      Volume: - Pages: 1-13

    • DOI

      10.1007/978-3-319-28275-6_62-1

    • ISBN
      9783319282756
    • Related Report
      2016 Research-status Report
  • [Presentation] 固体NMR法によるGM1ガングリオシド膜上におけるアミロイドβの構造解析2019

    • Author(s)
      矢木真穂,西村勝之, 加藤晃一
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 固体 NMR 法を用いたガングリオシド膜上におけるアミロイドβの構造解析2018

    • Author(s)
      矢木真穂,加藤晃一,西村勝之
    • Organizer
      第57回 NMR討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] NMR Characterization of Conformational Transition of Amyloid-β Promoted in Membrane Environments2016

    • Author(s)
      Maho Yagi-Utsumi, Katsuyuki Nishimura, Koichi Kato
    • Organizer
      27th International Conference on Magnetic Resonance in Biological Systems
    • Place of Presentation
      国立京都国際会館 (京都府 京都市)
    • Year and Date
      2016-08-21
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2022-02-21  

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