Cell type analysis of clonally related cortical neurons using initial axonal projection pattern

• Project/Area Number: 16K07011
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Osaka University (2018-2019); Chubu University (2017); National Institute for Physiological Sciences (2016)
• Principal Investigator: Hatanaka Yumiko 大阪大学, 生命機能研究科, 助教 (40271548)
• Project Period (FY): 2016-10-21 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 大脳皮質形成 / 発生・形態形成 / 細胞系譜 / 神経細胞分化 / ニューロンサブタイプ / 発生・分化 / 大脳皮質 / イメージング / 投射タイプ

Outline of Final Research Achievements

We first established the genetic methods for labeling cell lineages of the ventricular zone (VZ) cells and intermediate neuronal progenitors (INPs) in the developing mouse cerebral cortex. Then, we analyzed their daughter cell types, focusing on initial axonal projection pattern and cell type marker expression. Our analyses suggested that (1) VZ cells are heterogeneous for ability of producing neuronal types and number of neurons, and (2) INPs are mostly committed to produce pair cells sharing the same property. These results, together with future analyses clarifying the timeline for VZ cell differentiation, would help us to understand the mechanisms underlying the production of divergent neuronal types in the cerebral cortex.

Academic Significance and Societal Importance of the Research Achievements

”初期軸索投射”という独自の視点から2つに分類した皮質ニューロンタイプを元に、多様な皮質ニューロンが生じる基礎過程を細胞レベルで明らかにしようとした研究である。皮質ニューロンタイプは主としてその配置を元に、深層・浅層と大きく２つに分けて考えられてきたが、本研究では、ニューロンの機能に即した投射パターンを適応して１つの皮質幹細胞から生じるニューロンの分類を行った。解析の結果は、皮質幹細胞がニューロンタイプの産生に関して不均一であることを示唆しており、ニューロン多様性を生み出す機構解明の研究に関して１つの方向性を示すことが出来た。

Research Products

• [Presentation] Genetic labeling of neurons derived from direct and indirect neurogenesis in the mouse cerebral cortex (2020)
  • Author(s): Hatanaka Y.
  • Organizer: The 8th international neural microcircuit conference, Synaptic Specificity to Circuit Dynamics
  • Int'l Joint Research / Invited
• [Presentation] Genetic labeling of neurons derived from direct and indirect neurogenesis in the mouse cerebral cortex (2020)
  • Author(s): Hatanaka Y., Hirata T.
  • Organizer: New Frontier in Neurosciences 2020
  • Int'l Joint Research
• [Presentation] Genetic labeling of neurons derived from direct and indirect neurogenesis in the mouse cerebral cortex (2019)
  • Author(s): Hatanaka Y., Kawaguchi Y., Hirata T.
  • Organizer: Annual Meeting of Society for Neuroscience
  • Int'l Joint Research
• [Presentation] Labeling of neurons derived from intermediate neuronal progenitors in the mouse cerebral cortex (2018)
  • Author(s): Yumiko Hatanaka
  • Organizer: 新学術領域研究 スクラップ&ビルドによる脳機能の動的制御 第３回領域会議
• [Presentation] Labeling of neurons derived from intermediate neuronal progenitors in the mouse cerebral cortex (2017)
  • Author(s): 畠中由美子、平田たつみ、川口泰雄
  • Organizer: 第40回日本神経科学学会大会
• [Remarks] 大阪大学大学院 生命機能研究科 細胞分子神経生物学研究室（山本研究室）
  • URL: http://www.fbs.osaka-u.ac.jp/labs/neurobiol/