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The inhibition of autophagy potentiates anti-oncogenic and anti-angiogenic effect of sulforaphane by inducing apoptosis

Research Project

Project/Area Number 16K07161
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor therapeutics
Research InstitutionThe University of Tokyo

Principal Investigator

Nishikawa Takeshi  東京大学, 医学部附属病院, 助教 (00749799)

Co-Investigator(Kenkyū-buntansha) 石原 聡一郎  東京大学, 医学部附属病院, 教授 (00376443)
山口 博紀  東京大学, 医学部附属病院, 登録研究員 (20376445)
畑 啓介  東京大学, 医学部附属病院, 特任講師 (60526755)
渡邉 聡明  東京大学, 医学部附属病院, 教授 (80210920)
野澤 宏彰  東京大学, 医学部附属病院, 准教授 (80529173)
川合 一茂  東京大学, 医学部附属病院, 講師 (80571942)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsスルフォラファン / オートファジー / 血管内皮前駆細胞 / 大腸癌 / 血管新生 / 抗癌作用 / colon26 / アポトーシス / 抗がん物質探索 / ケミカルバイオロジー
Outline of Final Research Achievements

Sulforaphane (SUL) is a kind of isothiocyanate. We investigated the pro-apoptotic effect on endothelial progenitor cells (EPCs) and colon26. EPCs induced autophagy strongly even under normal condition and the inhibition of autophagy under the condition without SUL induced strongly apoptosis. The response of EPCs is different from that of endothelial cells, which induced autophagy in response to the pro-apoptotic agent, SUL and the inhibition of autophagy potentiated the pro-apoptotic effect.
On the other hand, similar to endothelial cells, colon26 induced autophagy in response to SUL and the inhibition of autophagy potentiated the pro-apoptotic effect. But the response of colon26 was weaker than that of endothelial cells. So, we tried to changed the exposure time against SUL, but we could not reach enough pro-apoptic effect of SUL by the inhibition of autophagy in colon26.

Academic Significance and Societal Importance of the Research Achievements

癌細胞および腫瘍新生血管内皮細胞が防御機構の一つとして誘導するオートファジーの阻害によりスルフォラファン(SUL)の抗腫瘍および抗血管新生効果は増強する。今回はマウスにおけるSULの抗腫瘍効果を確認した。しかし、もう一つの血管新生に関わる血管内皮前駆細胞におけるオートファジーは生存のために認められ、またマウス由来の大腸癌細胞株は十分なオートファジーを誘導できず十分な抗腫瘍効果を示せなかった。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2019 2018

All Journal Article (8 results) (of which Peer Reviewed: 7 results,  Open Access: 2 results)

  • [Journal Article] Association between KRAS G13D mutations and anastomotic recurrence in colorectal cancer: Two case reports.2019

    • Author(s)
      Okada S, Hata K, Kawai K, Yamamoto Y, Tanaka T, Nishikawa T, Sasaki K, Kaneko M, Emoto S, Murono K, Nozawa H.
    • Journal Title

      Medicine (Baltimore)

      Volume: 98

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Subtypes Are Frequently Discordant Between Lesions in Patients With Synchronous Colorectal Cancer: Molecular Analysis of 59 Patients2019

    • Author(s)
      ARAKAWA KEIICHI、HATA KEISUKE、NOZAWA HIROAKI、KAWAI KAZUSHIGE、TANAKA TOSHIAKI、NISHIKAWA TAKESHI、SASAKI KAZUHITO、SHUNO YASUTAKA、KANEKO MANABU、HIYOSHI MASAYA、EMOTO SHIGENOBU、MURONO KOJI、SONODA HIROFUMI、OKADA SATOSHI、ISHIHARA SOICHIRO
    • Journal Title

      Anticancer Research

      Volume: 39 Issue: 3 Pages: 1425-1432

    • DOI

      10.21873/anticanres.13258

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prognostic significance of doubling time in patients undergoing radical surgery for metachronous peritoneal metastases of colorectal cancer2019

    • Author(s)
      Miyake Hiroaki、Murono Koji、Nagata Hiroshi、Nozawa Hiroaki、Kawai Kazushige、Hata Keisuke、Tanaka Toshiaki、Nishikawa Takeshi、Shuno Yasutaka、Sasaki Kazuhito、Ishihara Soichiro
    • Journal Title

      International Journal of Colorectal Disease

      Volume: - Issue: 5 Pages: 801-809

    • DOI

      10.1007/s00384-019-03259-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Venous thromboembolism in colorectal surgery: Incidence, risk factors, and prophylaxis2019

    • Author(s)
      Emoto Shigenobu、Nozawa Hiroaki、Kawai Kazushige、Hata Keisuke、Tanaka Toshiaki、Shuno Yasutaka、Nishikawa Takeshi、Sasaki Kazuhito、Kaneko Manabu、Hiyoshi Masaya、Murono Koji、Ishihara Soichiro
    • Journal Title

      Asian Journal of Surgery

      Volume: - Issue: 9 Pages: 863-873

    • DOI

      10.1016/j.asjsur.2018.12.013

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The influence of neoadjuvant chemoradiation for lower rectal cancer on urinary function2019

    • Author(s)
      Hirata Yugo、Nozawa Hiroaki、Kawai Kazushige、Hata Keisuke、Tanaka Toshiaki、Nishikawa Takeshi、Otani Kensuke、Sasaki Kazuhito、Kaneko Manabu、Emoto Shigenobu
    • Journal Title

      Asian Journal of Surgery

      Volume: - Issue: 7 Pages: 731-739

    • DOI

      10.1016/j.asjsur.2018.11.004

    • Related Report
      2018 Annual Research Report
    • Open Access
  • [Journal Article] Neoadjuvant imatinib therapy in rectal gastrointestinal stromal tumors2018

    • Author(s)
      Kaneko Manabu、Emoto Shigenobu、Murono Koji、Sonoda Hirofumi、Hiyoshi Masaya、Sasaki Kazuhito、Shuno Yasutaka、Nishikawa Takeshi、Tanaka Toshiaki、Hata Keisuke、Kawai Kazushige、Nozawa Hiroaki
    • Journal Title

      Surgery Today

      Volume: - Issue: 6 Pages: 460-466

    • DOI

      10.1007/s00595-018-1737-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Safety of intraperitoneal paclitaxel combined with conventional chemotherapy for colorectal cancer with peritoneal carcinomatosis: a phase I trial2018

    • Author(s)
      Murono Koji、Nagata Hiroshi、Ishimaru Kazuhiro、Emoto Shigenobu、Kaneko Manabu、Hiyoshi Masaya、Sasaki Kazuhito、Otani Kensuke、Shuno Yasutaka、Nishikawa Takeshi、Tanaka Toshiaki、Hata Keisuke、Kawai Kazushige、Nozawa Hiroaki、Muro Kei、Ishihara Soichiro
    • Journal Title

      Cancer Chemotherapy and Pharmacology

      Volume: 83 Issue: 1 Pages: 145-150

    • DOI

      10.1007/s00280-018-3714-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correlations between the Recurrence Patterns and Sizes of Lateral Pelvic Lymph Nodes before and after Chemoradiotherapy in Patients with Lower Rectal Cancer2018

    • Author(s)
      Shiratori Hiroshi、Kawai Kazushige、Hata Keisuke、Tanaka Toshiaki、Nishikawa Takeshi、Sasaki Kazuhito、Kaneko Manabu、Murono Koji、Emoto Shigenobu、Morikawa Teppei、Fukayama Masashi、Nozawa Hiroaki
    • Journal Title

      Oncology

      Volume: 96 Issue: 1 Pages: 33-43

    • DOI

      10.1159/000492493

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed

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Published: 2016-04-21   Modified: 2020-03-30  

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