Functional analysis of P5 in glioblastoma cells for new molecular targeted therapy

• Project/Area Number: 16K07170
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor therapeutics
• Research Institution: Kyoto University
• Principal Investigator: Horibe Tomohisa 京都大学, 医学研究科, 特定准教授 (20467468)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: タンパク質 / 分子シャペロン / 癌 / ケミカルバイオロジー / 発光イメージング

Outline of Final Research Achievements

To elucidate the functional analysis of protein disulfide isomerase (PDI) P5 in glioblastoma cells, we performed the screening of binding proteins to P5 by immunoprecipitation method using affinity purified anti-P5 antibody, and we identified one of the binding proteins as vimentin. It was also found that the knockdown of P5 by siRNA in glioblastoma cells could affect the cell growth and migration. It is suggested that P5 has a significant role in glioblastoma cells and might be new target for the treatment.

Academic Significance and Societal Importance of the Research Achievements

細胞内で新しく作られたタンパク質の構造形成に関わる酵素の一つと考えられているP5について、細胞内における詳細な機能的役割については未だ不明である。本研究では、悪性脳腫瘍細胞内において、P5と結合するタンパク質を調べその一つがvimentinであることがわかり、また、P5が悪性脳腫瘍の増殖に重要な役割を担うと予想される研究結果を得た。本研究により、今後、悪性脳腫瘍における新しい標的分子の可能性およびその標的の有用性に関するさらなる研究が進むと予想される。

Research Products

• [Journal Article] Functional analysis of protein disulfide isomerase P5 in glioblastoma cells as a novel anti-cancer target (2019)
  • Author(s): Horibe, T., Torisawa, A., Masuda, Y., and Kawakami, K.
  • Journal Title: Oncology Rep Volume: 41 Pages: 961-972
  • DOI: 10.3892/or.2018.6868
  • Peer Reviewed
• [Presentation] Functional analysis of protein disulfide isomerase P5 in glioblastoma cells by bioluminescence imaging method at single cell level (2019)
  • Author(s): Horibe, T. and Kawakami, K.
  • Organizer: COMBIO 2018
  • Int'l Joint Research
• [Presentation] Functional analysis of protein disulfide isomerase P5 in glioblastoma cells as a potent anti-cancer target (2016)
  • Author(s): Horibe T and Kawakami K.
  • Organizer: 第75回日本癌学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-10-06