Is central area of Vietnam a new center of genetic diversity for genus Camellia? Evaluate genetic diversity and attempt for conservation.

• Project/Area Number: 16K07239
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservation of biological resources
• Research Institution: Osaka City University
• Principal Investigator: UEMATSU CHIYOMI 大阪市立大学, 大学院理学研究科, 准教授 (30232789)
• Co-Investigator(Kenkyū-buntansha): 片山 寛則 神戸大学, 農学研究科, 准教授 (50294202)
• Research Collaborator: HAKODA Naotoshi ; LUONG Van Dung ; LE NGUYET Hai Ninh ; NGUYEN Thi Lieu
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: ツバキ属植物 / ベトナム / 遺伝的多様性 / 集団構造解析 / Camellia / 保全

Outline of Final Research Achievements

In Genus Camellia, more than 250 species distribute in East and South-East Asia. But in Vietnam, number of species and its distribution were unclear because of war for a long time. By our 3 times explorations, 62 species, 598 individuals of wild Camellia were found and recorded from all over Vietnam. Among these accessions, 25 species, 29 populations originated from Southern area were analyzed using 13 SSR markers. Each population showed high genetic diversity, low allelic richness and thought to be highly homozygous. Result of AMOVA analysis indicated genetic differentiation among populations. Results of STRUCTURE analysis did not support the traditional taxonomic system. But some morphological traits in flower and leaf showed correlation with expected ancestral population. Thus these characteristics could be a key for species identification. More than 30 % of habitats are facing to the risk of environmental destruction, therefore conservation is urgently required.

Academic Significance and Societal Importance of the Research Achievements

ベトナム全土を網羅した学術調査は今回が初めてで、62種の分布を明らかにし、各集団の構造解析の結果に基づいて、保全が急務であることを示した。分子マーカーを用いた解析結果は従来の分類体系を支持しなかったが、葉と花のいくつかの形質が種の識別マーカーとなる可能性を示した点は、系統関係再考に重要な知見と考える。
ツバキ属植物は主にチャ、ツバキ油、鑑賞用に利用されてきたが、黄花種の薬用利用など、あらたな利用が増えている。ベトナムではチャ以外の種を飲用に供している地域もあった。今後に利用可能な遺伝資源として、早急な保全により絶滅を回避し、系統関係を明らかにすることが重要である。

Research Products

• [Int'l Joint Research] Da Lat University/VNU University of Science/Hoa Lu University(ベトナム)
• [Int'l Joint Research] Da Lat University/VNU University of Science/Hoa Lu University(ベトナム)
• [Int'l Joint Research] Da Lat 大学/Hoa Lu 大学/Hanoi 大学(ベトナム)
• [Journal Article] Two new species of Camellia (Theaceae) from Vietnam (2018)
  • Author(s): Lieu Thi Nguyen, Ninh Tran, Uematsu Chiyomi, Katayama Hironori, Dung Van Luong, Son Thanh Hoang, Ky Danh Nguyen, Hung Viet Nguyen, Toan Canh Thai
  • Journal Title: Korean Journal of Plant Taxonomy Volume: 48 Issue: 2 Pages: 115-122
  • DOI: 10.11110/kjpt.2018.48.2.115
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Camellia tuyenquangensis (Theaceae), a new species from Vietnam (2017)
  • Author(s): Le Ninh Nguyet Hai、Uematsu Chiyomi、Katayama Hironori、Nguyen Lieu Thi、Tran Ninh、Luong Dung Van、Hoang Son Thanh
  • Journal Title: Korean Journal of Plant Taxonomy Volume: 47 Issue: 2 Pages: 95-99
  • DOI: 10.11110/kjpt.2017.47.2.95
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Genetic relationships among Camellia species collected from Southern Vietnam (2018)
  • Author(s): T. L. Nguyen, K. Fukuyama, H. Katayama, C. Uematsu
  • Organizer: 日本育種学会
• [Presentation] ツバキ属遺伝資源の評価と系統関係の解明 (2018)
  • Author(s): 福山敬，片山寛則，植松千代美
  • Organizer: 日本育種学会
• [Presentation] Description of wild Camellia genetic resources collected from South of Vietnam. (2017)
  • Author(s): T. L. Nguyen, V. D. Luong, K. Fukuyama, H. Katayama, C. Uematsu
  • Organizer: 日本育種学会