Biochemical analysis of Human Mre11 complex working in DNA double-strand break repair
| Project/Area Number |
16K07255
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Osaka University (2017-2019)
Nara Institute of Science and Technology (2016) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
建部 恒 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (00596819)
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | DNA修復 / DNA二本鎖切断 / 高速AFM / 相同組換え / 二本鎖DNA切断 / Mre11 |
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| Outline of Final Research Achievements |
Human MRE11-RAD50-NBS1 (MRN) complex is a central player in protecting our genome from toxic DNA damages such as DNA double-strand break. In this project, we carried out biochemical and structural analyses of human MRN complex. We found that the basic properties of human MRN and its bacterial homolog SbcCD, including their enzymatic actions and entire architectures, are mostly well-conserved among species.
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| Academic Significance and Societal Importance of the Research Achievements |
MRN複合体はヒトの生存に必須の酵素複合体であり、ヒトのゲノムDNAの傷を修復し安定に維持するために働いている。本研究ではこのMRN複合体の蛋白質構造や酵素活性を詳細に調べ、その基礎的な知見を明らかにした。ヒトMRN複合体は発がんやがん治療のみならず、近年注目を集めるゲノム編集にも関係する重要な酵素であることから、本研究によりMRN複合体が働く仕組みの理解が深まったことは、医学・生物学的にも意義深いと考えられる。
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Report
(5 results)
Research Products
(13 results)
