| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Molecular mechanisms of COPII vesicle formation and its spatiotemporal regulation were analyzed, and the serine/threonine protein phosphatase2A was identified as a regulator of this reaction. Hyperphosphorylated form of Sec24 subunit was observed in the absence of this phosphatase, and this hyperphosphorylated Sec24 reduces its binding affinity to the ER membrane. Furthermore, Sec16 is shown to be incorporated within the COPII-cargo clusters, and that this is dependent on the Sar1 GTPase cycle.
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