The role of Rab10 in epithelial polarized transport

• Project/Area Number: 16K07348
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: Yoshimura Shin-ichiro 大阪大学, 医学系研究科, 講師 (60584521)
• Research Collaborator: Harada Akihiro
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: Rab / membrane traffic / Rab10 / TGN / リサイクリングエンドソーム / 極性輸送 / 上皮極性形成 / 膜輸送

Outline of Final Research Achievements

We identified a novel Rab10-binding protein with yeast two hybrid methods.
The Rab10-binding protein was localized on the trans-Golgi Network (TGN) and the recycling endosome (RE). Rab10-BP rapidly dissociated from TGN and RE membrane upon PI4K inhibitor, PAO treatment, suggesting that the association of Rab10-BP to the organelles were depend on either PI(4)P, PI(4,5)P2, or PI(3,4,5)P3. Rab10-BP-KD revealed that the molecule function is not only for the polarized traffics.

Academic Significance and Societal Importance of the Research Achievements

今回同定した分子は全く解析がされていないものであった。その機能の一旦を明らかにしたことは、研究コミュニティーへの大きな貢献であると考えている。本研究は細胞内輸送のメカニズムの全貌を明らかにするという壮大なプロジェクトのほんの一部分であるが、このような研究の積み重ねにより細胞とは、そして生命とは何かという問いに答えることができるのである。

Research Products

• [Journal Article] The Rab11-binding protein RELCH/KIAA1468 controls intracellular cholesterol distribution. (2018)
  • Author(s): Sobajima T., Yoshimura S*., Maeda T., Miyata H., Miyoshi E., and Harada A*. （*共同責任著者）
  • Journal Title: J Cell Biol Volume: pii: jcb.201709123 Issue: 5 Pages: 1-15
  • DOI: 10.1083/jcb.201709123
  • Peer Reviewed / Open Access
• [Journal Article] Opposing roles for SNAP23 in secretion in exocrine and endocrine pancreatic cells (2016)
  • Author(s): Kunii M, Ohara-Imaizumi M, Takahashi N, Kobayashi M, Kawakami R, Kondoh Y, Shimizu T, Simizu S, Lin B, Nunomura K, Aoyagi K, Ohno M, Ohmuraya M, Sato T, Yoshimura SI, Sato K, Harada R, Kim YJ, Osada H, Nemoto T, Kasai H, Kitamura T, Nagamatsu S, Harada A.
  • Journal Title: J Cell Biol. Volume: 215(1) Pages: 121-138
  • Peer Reviewed
• [Journal Article] EHBP1L1 coordinates Rab8 and Bin1 to regulate apical-directed transport in polarized epithelial cells. (2016)
  • Author(s): Nakajo A, Yoshimura SI, Togawa H, Kunii M, Iwano T, Izumi A, Noguchi Y, Watanabe A, Goto A, Sato T, Harada A.
  • Journal Title: Journal of Cell Biology Volume: 212 Issue: 3 Pages: 297-306
  • DOI: 10.1083/jcb.201508086
  • Peer Reviewed / Open Access
• [Presentation] EHBP1L1新規結合分子の同定 (2019)
  • Author(s): 吉村信一郎
  • Organizer: 第124回日本解剖学会総会・全国学術集会
• [Presentation] EHBP1L1 binds CD2AP. (2018)
  • Author(s): Shin-ichiro Yoshimura and Akihiro Harada
  • Organizer: Joint meeting of the 51th Annual meeting of the Japanese Society of the Developmental Biologist & the 70th Annual meeting of the Japanese Society for Cell biology.
• [Presentation] The molecular function of a novel Rab8-binding protein in polarized transport (2017)
  • Author(s): Shin-ichiro Yoshimura Atsuhiro Nakajo Akihiro Harada
  • Organizer: 第１２２回日本解剖学会全国学術集会
  • Place of Presentation: 長崎市
  • Year and Date: 2017-03-28
  • Invited