Studies on Functions of Transcription Factors That Regulate Assembly of Photosynthesis Machinery

• Project/Area Number: 16K07393
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: The University of Tokyo
• Principal Investigator: Masuda Tatsuru 東京大学, 大学院総合文化研究科, 教授 (00242305)
• Research Collaborator: SHIMIZU Takayuki
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 葉緑体 / クロロフィル / 転写因子 / 核コード光合成関連遺伝子 / 共発現 / 光合成 / 葉緑体形成 / 発現制御

Outline of Final Research Achievements

To elucidate the mechanism of coordinate transcriptional regulation of photosynthesis-associated nuclear genes (PhANGs) by multiple transcription factors, here we studied the functions of transcription factors that regulate the assembly of photosynthesis machinery. In summary, we demonstrated that GARP-type GLK and GATA-type GNC/CGA induce distinct PhANGs sets, suggesting both transcription factors distinctly regulate the assembly of photosystems. Furthermore, we have succeeded in prediction of novel GLK binding sequence.

Academic Significance and Societal Importance of the Research Achievements

本研究は、葉緑体分化および光合成装置形成の制御機構を明らかにする上で重要な学術的意義を持つだけでなく、植物に新たな光合成組織を創出する技術にも繋がり、将来における農学的な応用にも発展させることが期待できる。実際、葉緑体分化を促進させた植物体では、その光合成能力が向上するだけでなく、病虫害に対する抵抗性が高まることが最近明らかとなってきており、その応用価値は非常に高い。

Research Products

• [Journal Article] Galactolipids are essential for internal membrane transformation during etioplast-to-chloroplast differentiation (2019)
  • Author(s): Fujii Sho, Nagata Noriko, Masuda Tatsuru, Wada Hajime, Kobayashi Koichi
  • Journal Title: Plant and Cell Physiology Volume: 印刷中 Issue: 6 Pages: 1224-1238
  • DOI: 10.1093/pcp/pcz041
  • Peer Reviewed / Open Access
• [Journal Article] エチオプラストの発達と葉緑体への分化過程におけるガラクト脂質の役割 (2018)
  • Author(s): 藤井祥、永田典子、増田建、和田元、小林康一
  • Journal Title: 光合成研究 Volume: 28 Pages: 139-147
  • NAID: 40021773961
  • Peer Reviewed / Open Access
• [Journal Article] Monogalactosyldiacylglycerol facilitates synthesis of photoactive protochlorophyllide in etioplasts. (2017)
  • Author(s): Fujii, S., Kobayashi, K., Nagata, N., Masuda, T., and Wada, H.
  • Journal Title: Plant Physiology Volume: 174 Issue: 4 Pages: 2183-2198
  • DOI: 10.1104/pp.17.00304
  • Peer Reviewed
• [Journal Article] Shoot removal induces chloroplast development in roots via cytokinin signaling (2017)
  • Author(s): Koichi Kobayashi, Ai Ohnishi, Daichi Sasaki, Sho Fujii, Akira Iwase, Keiko Sugimoto, Tatsuru Masuda and Hajime Wada
  • Journal Title: Plant Physiology Volume: 173 Issue: 4 Pages: 2340-2355
  • DOI: 10.1104/pp.16.01368
  • Peer Reviewed / Open Access
• [Journal Article] Allocation of Heme Is Differentially Regulated by Ferrochelatase Isoforms in Arabidopsis Cells. (2016)
  • Author(s): Espinas, N. A., Kobayashi, K., Sato, Y., Mochizuki, N., Takahashi, K., Tanaka, R., & Masuda, T.
  • Journal Title: Frontiers in Plant Science Volume: 7 Pages: 1326-1326
  • DOI: 10.3389/fpls.2016.01326
  • NAID: 120005980877
  • Peer Reviewed / Open Access
• [Presentation] GUN1 regulates tetrapyrrole biosynthesis in Arabidopsis (2019)
  • Author(s): Masuda T, Shimizu T, Mochizuki N, Nagatani A, Watanabe S, Kacprzak S, Okamoto H, Terry MJ
  • Organizer: 第60回日本植物生理学会年会およびシンポジウム
• [Presentation] Tetrapyrrole-binding properties of GUN1 in Arabidopsis (2018)
  • Author(s): Shimizu T, Shimada T, Tanaka K, Mochizuki N, Watanabe S, Masuda T
  • Organizer: Gordon Research Conference: Chemistry and Biology of Tetrapyrroles
  • Int'l Joint Research
• [Presentation] Biochemical characterization of GUN1 in Arabidopsis (2018)
  • Author(s): Shimizu T, Mochizuki N, Watanabe S, Shimada T, Tanaka K, Hayashi Y, Arai M, Masuda T
  • Organizer: Conference Jacque-Monod: Retrograde signalling from emdosymbiotic organelles
• [Presentation] Cytokinin enhances photosystem assembly in Arabidopsis roots via transcriptional regulation (2018)
  • Author(s): Dwi Andi Listiawan, Takeshi Obayashi, Koichi Kobayashi and Tatsuru Masuda
  • Organizer: 第59回植物生理学会年会およびシンポジウム
• [Presentation] Biochemical characterization of GUN1 protein in Arabidopsis (2018)
  • Author(s): Takayuki Shimizu, Saumya Awasthi, Tomohiro Shimada, Kan Tanaka, Nobuyoshi Mochizuki, Satoru Watanabe and Tatsuru Masuda
  • Organizer: CSLS国際フォーラム
  • Int'l Joint Research
• [Presentation] Biochemical characterization of tetrapyrrole-binding pentatricopeptide-repeat (PPR) proteins in plastids (2017)
  • Author(s): Awasthi, S., Shimada, T., Tanaka, K., Mochizuki, N., Watanabe, S., Masuda, T.
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島大学（鹿児島県・鹿児島市）
  • Year and Date: 2017-03-16
• [Presentation] 可塑的な葉緑体分化における光合成系の構築 (2017)
  • Author(s): 増田 建
  • Organizer: 光合成科学：エネルギーとバイオマス
  • Place of Presentation: 東京工業大学（神奈川県・横浜市）
  • Int'l Joint Research / Invited
• [Book] 理系総合のための生命科学 第4版 (2018)
  • Author(s): 東京大学生命科学教科書編集委員会
  • Total Pages: 342
  • Publisher: 羊土社
  • ISBN: 9784758120869
• [Book] キャンベル生物学 第11版 (2018)
  • Author(s): 池内昌彦、伊藤元己、箸本春樹、道上達男監訳
  • Total Pages: 1643
  • Publisher: 丸善出版
  • ISBN: 9784621302767
• [Book] 科学の技法 東京大学「初年次ゼミナール理科」テキスト (2017)
  • Author(s): 増田建、坂口菊恵
  • Total Pages: 227
  • Publisher: 東京大学出版会
• [Remarks] 増田建研究室ホームページ
  • URL: http://webpark1435.sakura.ne.jp/wp/
• [Remarks] 増田建研究室ホームページ
  • URL: http://webpark1435.sakura.ne.jp/