Molecular mechanisms of reprogramming that is regulated by changes in endogenous phytohormone levels
Project/Area Number |
16K07398
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Plant molecular biology/Plant physiology
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Research Institution | Kyoto University |
Principal Investigator |
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Research Collaborator |
Ishida Sakiko
Suzuki Hidemasa
Hainiwa Emi
Yamaoka Shohei
Shigenobu Shuji
Sakakibara Hitoshi
Kohchi Takayuki
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Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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Keywords | リプログラミング / 再生 / オーキシン / サイトカイニン / 苔類ゼニゴケ / コケ植物 / ゼニゴケ / 幹細胞新生 / 脱分化 / RNA-seq / ホルモン定量 / 細胞周期再開 / 植物 / 発生・分化 / 進化 / 遺伝子 / シグナル伝達 |
Outline of Final Research Achievements |
In the liverwort Marchantia polymorpha, thallus regeneration occurs efficiently from excised thalli on phytohormone-free media. Based on the observations that the endogenous level of auxin decreased transiently a few hours after excision and that regenerant formation was repressed in the presence of auxin, transient reduction of auxin was suggested to trigger cellular reprogramming. In addition, genes for auxin and cytokinin biosynthesis and for a transcription factor were upregulated in auxin-reduction-dependent manner. Overexpression of this transcription factor promoted regenerant formation in the presence of auxin. This study revealed a relationship between changes in endogenous phytohormone levels and reprogramming of cells, and a key factor that links these two processes.
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Academic Significance and Societal Importance of the Research Achievements |
植物は挿し木で増やせることや、単離した1細胞から個体を再生できることから、分化全能性があると古くから知られている。傷害が引き金となって分化全能性が発揮されることはわかっているものの、その分子的な仕組みについての詳細はあまりわかっていなかった。本研究ではコケ植物のゼニゴケを用いて、葉状体切断後に起こる植物ホルモン・オーキシンの内生レベルの一時的な低下が、分化全能性を発揮するための遺伝子発現変化(リプログラミング)を引き起こすことを示唆する結果が得られた。この成果は、この分野に新たな概念をもたらすと考えられる。
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] Transcription factor DUO1 generated by neo-functionalization is associated with evolution of sperm differentiation in plants2018
Author(s)
Higo, A., Kawashima, T., Borg, M., Zhao, M., Lopez-Vidriero, I., Sakayama, H., et al. (他18名)
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Journal Title
Nature Communications
Volume: 9
Issue: 1
Pages: 5283-5283
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha.2018
Author(s)
Mano, S., Nishihama, R., Ishida, S., Hikino, K., Kondo, M., Nishimura, M., Yamato, T.K., Kohchi, K., and Nakagawa, T.
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Journal Title
PLOS ONE
Volume: 3
Issue: 10
Pages: e0204964-e0204964
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Loss of CG Methylation in Marchantia polymorpha Causes Disorganization of Cell Division and Reveals Unique DNA Methylation Regulatory Mechanisms of Non-CG Methylation.2018
Author(s)
Ikeda Y, Nishihama R, Yamaoka S, Arteaga-Vazquez MA, Aguilar-Cruz A, Grimanelli D, Pogorelcnik R, Martienssen RA, Yamato KT, Kohchi T, Hirayama T, Mathieu O.
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Journal Title
Plant Cell Physiol.
Volume: 12
Pages: 2421-2431
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Generative Cell Specification Requires Transcription Factors Evolutionarily Conserved in Land Plants2018
Author(s)
Yamaoka, S., Nishihama, R., Yoshitake, Y., Ishida, S., Inoue, K., Saito, M., Okahashi, K., Bao, H., Nishida, H., Yamaguchi, K., Shigenobu, S., Ishizaki, K., Yamato, K.T., and Kohchi, T.
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Journal Title
Current Biology
Volume: 28
Issue: 3
Pages: 479-486
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The Roles of the Sole Activator-Type Auxin Response Factor in Pattern Formation of Marchantia polymorpha2017
Author(s)
Kato, H., Kouno, M., Takeda, M., Suzuki, H., Ishizaki, K., Nishihama, R., and Kohchi, T.
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Journal Title
Plant and Cell Physiology
Volume: 58
Issue: 10
Pages: 1642-1651
DOI
Related Report
Peer Reviewed
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[Journal Article] Phytochrome signaling is mediated by PHYTOCHROME INTERACTING FACTOR in the liverwort Marchantia polymorpha2016
Author(s)
Inoue, K., Nishihama, R., Kataoka, H., Hosaka, M., Manabe, R., Nomoto, M., Tada, Y., Ishizaki, K., and Kohchi, T.
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Journal Title
Plant Cell
Volume: 28
Issue: 6
Pages: 1406-1421
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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