Roles of ACTIN DEPOLYMERIZING FACTOR in regulations of nuclear morphology and gene expression during plant response against pathogen attack.

• Project/Area Number: 16K07415
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Osaka Prefecture University (2018); Nara Institute of Science and Technology (2016-2017)
• Principal Investigator: Inada Noriko 大阪府立大学, 生命環境科学研究科, 准教授 (30432595)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: アクチン脱重合因子 / 細胞核構造 / ヘテロクロマチン / マイクロアレイ解析 / 病害応答 / アブラナ科炭疽病 / 植物病害応答 / 遺伝子発現制御 / 細胞骨格 / イメージング / マイクロアレイ

Outline of Final Research Achievements

Based on my previous study, I have proposed that Arabidopsis thaliana ACTIN DEPOLYMERIZING FACTOR (ADF) regulates gene expression during plant response against pathogen attack through regulation of organization or dynamics of nuclear actin microfilaments. To test this hypothesis, I conducted this study and have revealed that: (1) nuclear morphology was altered in adf mutants; (2) many genes that were differently regulated in adf mutants showed clusters in the chromosomes; (2) adf mutants shows increased resistance against Colletotrichum pathogen in addition to powdery mildew.

Academic Significance and Societal Importance of the Research Achievements

細胞表層で細胞形態や細胞内の物質輸送に働くアクチン繊維は、細胞核内にも存在することが知られていたものの、その機能は長い間謎であった。本研究では、ADFの細胞核内の働きに焦点を当てて解析を進めることにより、遺伝子発現制御という細胞核内アクチン繊維の新たな役割を示唆する結果を得た。この成果は、学術的にはアクチン繊維の新機能、遺伝子発現の新制御機構を明らかにする基盤研究としての意義を持つ。さらに本研究では、シロイヌナズナのadf変異体が複数の病原体に対して抵抗性を亢進させることを明らかにした。これは、病原体抵抗性植物の作出という、社会的に大きな意義を持つ応用研究に直接結びつく重要な成果である。

Research Products

• [Journal Article] Temperature imaging using a cationic linear fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy (2019)
  • Author(s): Noriko Inada, Nanaho Fukuda, Teruyuki Hayashi, Seiichi Uchiyama
  • Journal Title: Nature Protocols Volume: 14 Issue: 4 Pages: 1293-1321
  • DOI: 10.1038/s41596-019-0145-7
  • Peer Reviewed
• [Journal Article] In vivo monitoring of plant small GTPase activation using a F?rster resonance energy transfer biosensor (2018)
  • Author(s): Wong Hann Ling、Akamatsu Akira、Wang Qiong、Higuchi Masayuki、Matsuda Tomonori、Okuda Jun、Kosami Ken-ichi、Inada Noriko、Kawasaki Tsutomu、Kaneko-Kawano Takako、Nagawa Shingo、Tan Li、Kawano Yoji、Shimamoto Ko
  • Journal Title: Plant Methods Volume: 14 Issue: 1 Pages: 56-56
  • DOI: 10.1186/s13007-018-0325-4
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A Cell-Targeted Non-Cytotoxic Fluorescent Nanogel Thermometer Created with an Imidazolium-Containing Cationic Radical Initiator. (2018)
  • Author(s): Uchiyama, S., Tsuji, T., Kawamoto, K., Okano, K., Fukatsu, E., Noro, T., Ikado, K., Yamada, S., Shibata, Y., Hayashi, T., Inada, N., Kato, M., Koizumi, H., and Tokuyama, H.
  • Journal Title: Angewandte Chemie Volume: 57 Issue: 19 Pages: 5413-5417
  • DOI: 10.1002/anie.201801495
  • Peer Reviewed / Open Access
• [Journal Article] The Plant Cytoskeletons in Interactions between Plants and Obligate Biotrophs (2017)
  • Author(s): Inada, N.
  • Journal Title: CYTOLOGIA Volume: 82 Issue: 4 Pages: 341-348
  • DOI: 10.1508/cytologia.82.341
  • NAID: 130006250988
  • ISSN: 0011-4545, 1348-7019
  • Peer Reviewed / Open Access
• [Journal Article] Intracellular temperature measurements with fluorescent polymeric thermometers. (2017)
  • Author(s): Uchiyama, S., Gota, C., Tsuji, T., and Inada, N.
  • Journal Title: Chemical Communications Volume: 53 Issue: 80 Pages: 10976-10992
  • DOI: 10.1039/c7cc06203f
  • Peer Reviewed / Open Access
• [Journal Article] Constitutive activation of plant-specific RAB5 GTPase confers increased resistance against adapted powdery mildew fungus (2017)
  • Author(s): Inada, N., Ebine, K., Ito, E., Nakano, A., and Ueda, T.
  • Journal Title: Plant Biotechnology Volume: 34 Issue: 2 Pages: 89-95
  • DOI: 10.5511/plantbiotechnology.17.0501a
  • NAID: 130006789286
  • ISSN: 1342-4580, 1347-6114
  • Peer Reviewed / Open Access
• [Journal Article] Plant Actin Depolymerizing Factor Actin microfilament disassembly and more. (2017)
  • Author(s): Inada, N.
  • Journal Title: Journal of Plant Research Volume: 130 Issue: 2 Pages: 227-238
  • DOI: 10.1007/s10265-016-0899-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Calcium ions function as a booster of chromosome condensation. (2016)
  • Author(s): Phengchat, R., Takata, H., Morii, K., Inada, N., Murakoshi, H., Uchiyama, S., and Fukui, K.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 38281-38281
  • DOI: 10.1038/srep38281
  • NAID: 120006995007
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] DRP1-dependent Endocytosis Is Essential for Polar Localization and Boron-induced Degradation of the Borate Transporter BOR1 in Arabidopsis thaliana (2016)
  • Author(s): Yoshinari, A., Fujimoto, M., Ueda, T., Inada, N., Naito, S. and Takano, J.
  • Journal Title: Plant Cell Phys. Volume: 57 Issue: 9 Pages: 1985-2000
  • DOI: 10.1093/pcp/pcw121
  • Peer Reviewed
• [Journal Article] Modulation of plant RAB GTPase-mediated membrane trafficking pathway at the interface between plants and obligate biotrophic pathogens. (2016)
  • Author(s): Inada, N., Betsuyaku, S., Shimada, T., Ebine, K., Ito, E., Kutsuna, N., Hasezawa, S., Takano, Y., Fukuda, H., Nakano, A., & Ueda, T.
  • Journal Title: Plant Cell Physiol. Volume: 57 Issue: 9 Pages: 1854-1864
  • DOI: 10.1093/pcp/pcw107
  • Peer Reviewed / Open Access
• [Journal Article] Visualization of host actin microfilament dynamicity upon obligate biotrophic pathogen infection. (2016)
  • Author(s): Inada, N.
  • Journal Title: Cytologia Volume: 81 Pages: 119-120
  • NAID: 130005159788
  • Peer Reviewed
• [Presentation] レシオ型蛍光性温度センサーによる褐色脂肪細胞の温度計測 (2018)
  • Author(s): 内山聖一、辻俊一、井門久美子、河本恭子、稲田のりこ
  • Organizer: 第78回分析化学討論会
• [Presentation] 病原体応答におけるシロイヌナズナアクチン脱重合因子の機能解析 (2018)
  • Author(s): 稲田のりこ、梅田正明
  • Organizer: 日本植物生理学会
• [Presentation] アクチン脱重合因子による遺伝子発現制御機構の解析 (2017)
  • Author(s): 稲田のりこ、坂本卓也、松永幸大、梅田正明
  • Organizer: 日本植物学会
• [Presentation] 病害応答における植物アクチン脱重合因子の機能解析 (2016)
  • Author(s): 稲田のりこ、桧垣匠、馳澤盛一郎
  • Organizer: 日本植物学会第80回大会
  • Place of Presentation: 沖縄コンベンション・センター（沖縄県宜野湾市）
  • Year and Date: 2016-09-16
• [Presentation] 病害応答における植物アクチン脱重合因子の機能解析 (2016)
  • Author(s): 稲田のりこ、桧垣匠、馳澤盛一郎
  • Organizer: 日本植物形態学会第28回大会
  • Place of Presentation: 琉球大学（沖縄県中頭郡）
  • Year and Date: 2016-09-15
• [Presentation] シロイヌナズナのホウ酸輸送隊BOR1の偏在と分解を制御するエンドサイトーシス機構 (2016)
  • Author(s): 吉成晃、藤本優、天野太郎、山岡尚平、嶋田知生、上田貴志、稲田のりこ、西村いくこ、内藤哲、高野順平
  • Organizer: 第11回トランスポーター研究会年会
  • Place of Presentation: 京都大学宇治キャンパス（京都府宇治市）
  • Year and Date: 2016-07-02
• [Presentation] 感温性高分子を利用した細胞内温度計測用レシオ型蛍光プローブ (2016)
  • Author(s): 内山聖一、辻俊一、井門久美子、林晃之、稲田のりこ
  • Organizer: 第65回高分子学会年次大会
  • Place of Presentation: 神戸国際会議場（兵庫県神戸市）
  • Year and Date: 2016-05-25