Understanding the evolution of metazoan multicellularity by using the expanded premetazoan models

• Project/Area Number: 16K07468
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Prefectural University of Hiroshima
• Principal Investigator: Suga Hiroshi 県立広島大学, 生命環境学部, 准教授 (30734107)
• Research Collaborator: Ruiz-Trillo Iñaki ; Miller Todd
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 多細胞性進化 / 単細胞ホロゾア / 多細胞性 / 進化 / 細胞接着 / 細胞連絡 / 多細胞 / 細胞間連絡 / ゲノム

Outline of Final Research Achievements

The molecular mechanism that enabled the evolution of animal multicellularity has yet to be uncovered. We established and expanded the repertoire of “unicellular holozoan”models, the closest unicellular relatives to animals, by sequencing their genomes and transcriptomes and by developing the means to analyze the functions of their "multicellular" genes.

Academic Significance and Societal Importance of the Research Achievements

生物の設計図であるゲノムが解読され（1次元）、その働きかたを示すトランスクリプトーム（2次元）が明らかとなるとともに、こうした情報を実際の生物の体の作られ方に当てはめて捉えよう（3次元）という動きが活発です。比較的最近盛んになってきた進化発生学（本研究もその一つです）では、それを時間軸に沿って拡張し（4次元）、生物の進化の仕組みを遺伝子レベルで解き明かそうとしています。本研究は、特に動物が多細胞体制を進化させた「多細胞化」というイベントに注目し、動物に最も近い親戚である単細胞ホロゾアと呼ばれる単細胞生物群の研究を通じて、生物進化の仕組みの一部を解き明かそうとするものです。

Research Products

• [Int'l Joint Research] Institute of Evolutionary Biology/Universitat Pompeu Fabra/CSIC(スペイン)
• [Int'l Joint Research] Oslo University(ノルウェー)
• [Int'l Joint Research] New York State University/Dartmouth College(米国)
• [Int'l Joint Research] University of Bristol(英国)
• [Int'l Joint Research] Stockholm University(スウェーデン)
• [Int'l Joint Research] Institute de Biologia Evoltiva(Spain)
• [Int'l Joint Research] Stony Brook University(米国)
• [Int'l Joint Research] Dalhousie University(Canada)
• [Int'l Joint Research] Institut de Biologia Evolutiva/Universitat de Barcelona/Universitat de Pompeu Fabra(スペイン)
• [Int'l Joint Research] Stony Brook University(米国)
• [Journal Article] Revision of the Capsaspora genome using read mating information adjusts the view on premetazoan genome (2018)
  • Author(s): Denbo Seitaro、Aono Katsutoshi、Kai Takaaki、Yagasaki Rei、Ruiz-Trillo I?aki、Suga Hiroshi
  • Journal Title: Development, Growth & Differentiation Volume: 61 Issue: 1 Pages: 34-42
  • DOI: 10.1111/dgd.12587
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Unicellular Origin of the Animal MicroRNA Machinery (2018)
  • Author(s): Br?te Jon、Neumann Ralf S.、Fromm Bastian、Haraldsen Arthur A.B.、Tarver James E.、Suga Hiroshi、Donoghue Philip C.J.、Peterson Kevin J.、Ruiz-Trillo I?aki、Grini Paul E.、Shalchian-Tabrizi Kamran
  • Journal Title: Current Biology Volume: 28 Issue: 20 Pages: 3288-3295.e5
  • DOI: 10.1016/j.cub.2018.08.018
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Transfection ofCapsaspora owczarzaki, a close unicellular relative of animals (2018)
  • Author(s): Parra-Acero Helena、Ros-Rocher N?ria、Perez-Posada Alberto、Ko?yczkowska Aleksandra、S?nchez-Pons N?ria、Nakata Azusa、Suga Hiroshi、Najle Sebasti?n R.、Ruiz-Trillo I?aki
  • Journal Title: Development Volume: 145 Issue: 10 Pages: 162107-162107
  • DOI: 10.1242/dev.162107
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Src signaling in a low-complexity unicellular kinome (2018)
  • Author(s): Suga Hiroshi、Miller W. Todd
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 5362-5362
  • DOI: 10.1038/s41598-018-23721-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Historical geography of urushi, a Japanese traditional lacquer, in the Miyoshi area, Hiroshima (2018)
  • Author(s): Takeda, K. and Suga, H.
  • Journal Title: J Life Environ Sci Volume: 10 Pages: 13-23
  • NAID: 40021560001
  • Peer Reviewed
• [Journal Article] Extreme genome diversity in the hyper-prevalent parasitic eukaryote Blastocystis (2017)
  • Author(s): Gentekaki, E., Curtis, B. A., Stairs, C., Klimes, V., Elias, M., Salas, D., Herman, E. K., Eme, L., Arias, M. C., Henrissat, B., Hilliou, F., Klute, M. J., Suga et al.
  • Journal Title: PLoS Biology Volume: 15 Issue: 9 Pages: e2003769-e2003769
  • DOI: 10.1371/journal.pbio.2003769
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Dynamics of genomic innovation in the unicellular ancestry of animals (2017)
  • Author(s): Grau-Bove, X., Torruella, G., Donachie, S., Suga, H., Leonard, G., Richards, T. A., Ruiz-Trillo, I
  • Journal Title: eLife Volume: 6
  • DOI: 10.7554/elife.26036
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Possibility of RhoGDIβ as a molecular target for biomedical applications (2016)
  • Author(s): Tatsuka, M., Fujiwara, M., Okamoto, M., Hori, M., Sugihara, Y., Jikihara, H., Nakaoji, K., Hamada, K., Suga, H., and Shimamoto, F.
  • Journal Title: J Life Environ Sci Volume: 8 Pages: 1-12
  • NAID: 40020804616
  • Peer Reviewed
• [Journal Article] Radiation-induced RhoGDIβ cleavage leads to perturbation of cell polarity: a possible link to cancer spreading (2016)
  • Author(s): Fujiwara, M., Okamoto, M., Hori, M., Suga, H., Jikihara, H., Sugihara, Y., Shimamoto, F., Mori, T., Nakaoji, K., Hamada, K., Ota, T., Wiedemuth, R., Temme, A., and Tatsuka, M.
  • Journal Title: J Cell Physiol Volume: 0 Issue: 11 Pages: 0-0
  • DOI: 10.1002/jcp.25362
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] The origin of cell adhesion and communication: a view from unicellular holozoans (2018)
  • Author(s): Hiroshi Suga
  • Organizer: Joint Annual Meeting of 51st Japanese Society of Developmental Biology and Japan Society for Cell Biology
  • Int'l Joint Research / Invited
• [Presentation] Transition from protists to animals: the evolution of metazoan multicellularity (2018)
  • Author(s): Hiroshi Suga
  • Organizer: The 89th Annual Meeting of Zoological Science of Japan
  • Int'l Joint Research / Invited
• [Presentation] The evolution of multicellularity - a unicellular holozoan point of view (2018)
  • Author(s): Hiroshi Suga
  • Organizer: The 46th Naito Conference “Mechanisms of evolution and biodiversity”
  • Int'l Joint Research
• [Presentation] 多細胞システムの胎動 - 単細胞ホロゾアから探る多細胞性進化 (2017)
  • Author(s): 菅裕
  • Organizer: 日本進化学会
  • Invited
• [Presentation] 単細胞ホロゾアから探る多細胞性の進化 (2017)
  • Author(s): 菅裕
  • Organizer: 日本遺伝学研究所研究集会
  • Invited
• [Presentation] 22.Unicellular holozoans: new models revealing the mechanisms of multicellularity evolution (2016)
  • Author(s): Hiroshi Suga
  • Organizer: The 22th International Congress of Zoology
  • Place of Presentation: Okinawa
  • Year and Date: 2016-11-17
  • Int'l Joint Research / Invited
• [Presentation] 単細胞生物が持つ「多細胞的な」遺伝子の機能 (2016)
  • Author(s): 菅裕
  • Organizer: 日本進化学会
  • Place of Presentation: 東京工業大学大岡山キャンパス
  • Year and Date: 2016-08-25
• [Remarks] Suga lab home page
  • URL: http://www.pu-hiroshima.ac.jp/~hsuga/
• [Remarks] Genomic Evodevo Lab
• [Remarks] Suga Lab Homepage
  • URL: http://www.pu-hiroshima.ac.jp/~hsuga