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Community structure of genus Skeletonema in coastal waters

Research Project

Project/Area Number 16K07508
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ecology/Environment
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

Katano Toshiya  東京海洋大学, 学術研究院, 准教授 (00509820)

Research Collaborator Yamada Machiko  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords珪藻赤潮 / Skeletonema / 競争 / 群集組成 / 種組成解析 / 休眠期細胞 / 定量PCR / 季節変化 / 種間競争 / 珪藻 / Skeletonema属 / PCR / 種遷移 / Skeletonema属珪藻 / 種組成 / リアルタイムPCR / マルチプレックスPCR / FISH法
Outline of Final Research Achievements

Primer sets against 10 Skeletonema species for Real time PCR and multiplex PCR were developed. Species composition of Skeletonema in Tokyo Bay and the Ariake Sea was evaluated using these PCR techniques. In both bays, more than 6 species of Skeletonema including freshwater species of S. potamos were detected. Especially in the bottom sediment of the rivers inflowing to the bays, we detected high abundance and many species of Skeletonema were detected. These results imply that bottom sediments of these rivers possibly play important role as the seed bank of Skeletonema bloom in estuaries. In the competition between winter dominant species of S. japonicum and S. marinoi-dohrnii complex, in addition to environmental factors, endogenous factor (valve diameter) strongly affects on the results of the competition.

Academic Significance and Societal Importance of the Research Achievements

珪藻Skeletonema属は、世界中の多くの内湾に出現し、とりわけ富栄養な水域で優占することが知られている。東京湾とアメリカのナラガンセット湾ではどちらも植物プランクトンの90%以上が、Skeletonemaとされる。2005、2007年のSarnoらの発表によりS. costatumは、8種にも分類された。この新しい分類体系に基づく種同定は、単離と単離株の種同定に依存し多大な労力と時間を要していたが、本研究で開発されたプライマーセットにより迅速な種判別および定量解析が可能になった。世界の内湾で季節を問わず赤潮を起こすSkeletonemaの動態について今後大きく研究が進むことが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Development of primer sets for a multiplex and a qPCR assays targeting Skeletonema species and its application to field samples2019

    • Author(s)
      1.Enjoji, N. Katano, T., Yoshinaka, Y., Furuoka, F., Ando, Y., Yamada, M., Hamasaki, T., Miyamura, E., Otsubo, M., Yokoyama, K.
    • Journal Title

      Journal of Oceanography

      Volume: in press Issue: 4 Pages: 319-334

    • DOI

      10.1007/s10872-018-00504-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of cell size on growth rates of two Skeletonema species and their competitive interactions2018

    • Author(s)
      Ando, Y. Katano, T.
    • Journal Title

      Journal of Phycology

      Volume: 54 Issue: 6 Pages: 850-859

    • DOI

      10.1111/jpy.12782

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 東京湾における珪藻Skeletonema属のPCR法による出現種解析2018

    • Author(s)
      吉中祐紀,片野俊也
    • Organizer
      日本プランクトン・ベントス合同大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 東京湾の底泥中における珪藻Skeletonema属の qPCR による定量解析2018

    • Author(s)
      古岡鳳夏,片野俊也
    • Organizer
      日本プランクトン・ベントス合同大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 河川感潮域から河口域における底泥堆積物と水柱のSkeletonema属珪藻の種組成解析2018

    • Author(s)
      片野俊也、円城寺夏実、山田真知子、大坪繭子、中島有紀子、横山勝英、吉野健児
    • Organizer
      日本地球惑星科学連合2018年大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 東京湾におけるSkeletonema属珪藻のPCR法による出現種解析2017

    • Author(s)
      吉中 祐紀、小林あずさ、円城寺夏実、片野俊也
    • Organizer
      海洋学会 海洋生物学研究会シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2017-03-21
    • Related Report
      2016 Research-status Report
  • [Presentation] 細胞サイズの減少ー回復過程が珪藻の増殖速度と競争の結果に及ぼす影響2017

    • Author(s)
      安東祐太朗、片野俊也
    • Organizer
      日本プランクトン学会ベントス学会合同大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Development of multiplex and real-time PCR assays for the detection and quantification of Skeletonema species2017

    • Author(s)
      T Katano, N Enjoji, Y Yoshinaka, F Furuoka, Y Ando, M Yamada, M Otsubo, K Yokoyama
    • Organizer
      10th EASTHAB Symposium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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