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Cloning and characterization of blast resistance QTL qBRM6.2 in rice

Research Project

Project/Area Number 16K07549
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionHokkaido University

Principal Investigator

Inukai Tsuyoshi  北海道大学, 農学研究院, 講師 (90223239)

Research Collaborator KATO miyako  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsイネ / いもち病 / 圃場抵抗性 / 植物免疫 / 病害抵抗性 / 水稲 / 真性抵抗性 / クローニング / 育種学 / 遺伝子 / ストレス
Outline of Final Research Achievements

In the present study, we focused on qBRM6.2, a known blast-resistance QTL in experimental resistant rice line MC276. Because it was possible that Pid3-I1, one of the alleles at the Pid3 locus might be qBRM6.2 according to the previous results, we investigated the resistance phenotype of Pid3-I1 to Japanese isolates using Nipponbare transgenic lines that express Pid3-I1. The results showed that Pid3-I1 was a race-specific but partial-resistance allele at the Pid3 locus, suggesting strongly that Pid3-I1 is qBRM6.2.

Academic Significance and Societal Importance of the Research Achievements

真性抵抗性遺伝子として報告されていたPid3-I1遺伝子が実際は圃場抵抗性遺伝子であること及びその特性が明らかになったことで、抵抗性育種におけるこの遺伝子の利用において有用な知見が得られた。また、配列のわずかな違いによって真性抵抗性(Pid3-I3遺伝子)から圃場抵抗性(Pid3-I1遺伝子)へと形質が変わることが明らかとなり、今後圃場抵抗性の機構を解明する上で重要な手がかりが得られた。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (2 results)

All 2019 2018

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Pid3-I1 is a race specific partial resistance allele at the Pid3 blast resistance locus in rice2019

    • Author(s)
      Tsuyoshi Inukai, Saki Nagashima, Miyako Kato
    • Journal Title

      Theoretical and Applied Genetics

      Volume: 132 Issue: 2 Pages: 395-404

    • DOI

      10.1007/s00122-018-3227-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] イネいもち病真性抵抗性に関わるPid3座に分化した複対立遺伝子の一つは量的抵抗性に関与する2018

    • Author(s)
      長嶋 沙希, 加藤 美弥子, 犬飼 剛
    • Organizer
      日本育種学会
    • Related Report
      2017 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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