Identification and use of soybean gene for downy mildew resistance
Project/Area Number |
16K07565
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Science in genetics and breeding
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Research Institution | National Agriculture and Food Research Organization |
Principal Investigator |
TAGUCHI-SHIOBARA Fumio 国立研究開発法人農業・食品産業技術総合研究機構, 次世代作物開発研究センター, 上級研究員 (10355753)
|
Project Period (FY) |
2016-04-01 – 2019-03-31
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Project Status |
Completed (Fiscal Year 2018)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | ダイズ / 耐病性 / QTL / ダイズべと病 / マーカー / 育種学 / ゲノム / 農林水産物 / 植物 |
Outline of Final Research Achievements |
Downy mildew reduces soybean seed quality and size. It is a problem in Japan, where 90% of soybean grown is used as food. In the USA, 33 downy mildew races have been reported, but race differentiation in Japan is unclear. To identify quantitative trait loci (QTL) for downy mildew resistance effective in the Kanto and Tohoku regions, we performed QTL analysis using five populations of recombinant inbred lines (RILs). We evaluated spontaneously developed symptoms of the RIL. QTLs which were detected in both Kanto and Tohoku regions were verified by evaluating recombinant fixed pairs or introgression line in both regions. In summary, two QTL on chromosomes 7 and 18, which were detected in the population originated from ‘Natto-shoryu’ × ‘Tachinagaha’, and one QTL on chromosome 8, which was detected in the population originated from ‘Satonohohoemi’ × ‘Fukuibuki’, were confirmed to be effective in both Tohoku and Kanto regions.
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Academic Significance and Societal Importance of the Research Achievements |
枝豆育種は都道府県の公設試では無く種苗会社が担っているが、今回明らかになった関東~東北地方でダイズべと病抵抗性を付与できるQTL近傍のDNAマーカーは、育種での個体あるいは系統を選抜する際に活用できると考えられる。まだQTLの座乗領域が数Mbと広いが、本研究で作成された組換え固定系統対を用いてQTLの座乗領域を狭めていけば、実用的なDNAマーカーを得ることができる。
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Report
(4 results)
Research Products
(2 results)