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Regulatory mechanism of disrupting host cell nucleus by producing R-bodies

Research Project

Project/Area Number 16K07638
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionThe University of Tokyo

Principal Investigator

Aono Toshihiro  東京大学, 生物生産工学研究センター, 講師 (10372418)

Co-Investigator(Kenkyū-buntansha) 齋藤 勝晴  信州大学, 学術研究院農学系, 准教授 (40444244)
Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords根粒菌 / 共生 / 病原性 / 病原菌 / R-body / reb遺伝子 / reb遺伝子群 / rebオペロン / ampC / ベータラクタマーゼ / 温度
Outline of Final Research Achievements

Azorhizobium caulinodans is a rhizobium of a tropical legume Sesbania rostrata that forms nitrogen-fixing nodules on the roots and stems. This bacterium fixes nitrogen in the host cells in the stem/root nodules and provide ammonium to the host plants. Although A. caulinodans is a symbiotic bacterium, it has the ability to produce a host cell killing factor called R-body. Therefore, this bacterium establishes symbiosis with the host by strongly suppressing the expression of the reb genes that are involved in R-body synthesis. In the present study, we elucidated the molecular mechanism of the expression of reb genes.

Academic Significance and Societal Importance of the Research Achievements

R-bodyという因子は元来、ゾウリムシの内生細菌が宿主ゾウリムシを殺傷するために持つものと長い間認識されてきた。しかし、我々は、このR-bodyによる真核生物に対する病原性は、ゾウリムシ内生菌だけではなく、マメ科植物と相利共生を示す根粒菌においても発揮されるということを明らかにしてきた。本研究を含めた我々の近年の成果は、その病原性の制御機構を全細菌で初めて示したものであり、細菌学上、重要な位置づけにあると考えられる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2019 2018 2017 2016

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (5 results)

  • [Journal Article] Localization of the <i>reb</i> operon expression is inconsistent with that of the R-body production in the stem nodules formed by <i>Azorhizobium caulinodans</i> mutants having a deletion of <i>praR</i>2019

    • Author(s)
      Matsuoka Jun-ichi、Ishizuna Fumiko、Ogawa Tetsuhiro、Hidaka Makoto、Siarot Lowela、Aono Toshihiro
    • Journal Title

      The Journal of General and Applied Microbiology

      Volume: 65 Issue: 4 Pages: 209-213

    • DOI

      10.2323/jgam.2018.09.003

    • NAID

      130007706592

    • ISSN
      0022-1260, 1349-8037
    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Stringent Expression Control of Pathogenic R-body Production in Legume Symbiont Azorhizobium caulinodans2017

    • Author(s)
      Matsuoka Jun-ichi、Ishizuna Fumiko、Kurumisawa Keigo、Morohashi Kengo、Ogawa Tetsuhiro、Hidaka Makoto、Saito Katsuharu、Ezawa Tatsuhiro、Aono Toshihiro
    • Journal Title

      mBio

      Volume: 8 Issue: 4

    • DOI

      10.1128/mbio.00715-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Novel Regulatory Pathway for K + Uptake in the Legume Symbiont Azorhizobium caulinodans in Which TrkJ Represses the kdpFABC Operon at High Extracellular K + Concentrations2017

    • Author(s)
      Siarot Lowela、Toyazaki Hiroki、Hidaka Makoto、Kurumisawa Keigo、Hirakawa Tomoki、Morohashi Kengo、Aono Toshihiro
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 83 Issue: 19

    • DOI

      10.1128/aem.01197-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] A putative TetR-type transcription factor AZC_3265 from the legume symbiont Azorhizobium caulinodans represses the production of R-bodies that are toxic to eukaryotic cells2017

    • Author(s)
      Matsuoka Jun-ichi、Ishizuna Fumiko、Kurumisawa Keigo、Morohashi Kengo、Ogawa Tetsuhiro、Hidaka Makoto、Saito Katsuharu、Ezawa Tatsuhiro、Aono Toshihiro
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 63 Issue: 5 Pages: 452-459

    • DOI

      10.1080/00380768.2017.1371571

    • NAID

      210000011925

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] AmpRを介して温度により制御されるセスバニア根粒菌の宿主殺傷能と抗生物質耐性能2019

    • Author(s)
      平川智基、松岡淳一、諸橋賢吾、青野俊裕
    • Organizer
      日本土壌肥料学会2019年度静岡大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] セスバニア根粒菌の宿主殺傷能とβ-lactam 系抗生物質耐性能は 転写因子 AmpR を介して温度により制御される2019

    • Author(s)
      平川智基、松岡淳一、諸橋賢吾、青野俊裕
    • Organizer
      第12回北陸合同バイオシンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] セスバニア根粒菌における宿主殺傷能とアンピシリン耐性能は転写因子AmpRを介して温度により制御される2018

    • Author(s)
      平川智基、松岡淳一、日高真誠、諸橋賢吾、青野俊裕
    • Organizer
      日本農芸化学会関東支部2018年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] セスバニア根粒菌の病原性R-bodyオペロンとアンピシリン耐性遺伝子の発現は転写因子AmpRを介して温度により制御される2018

    • Author(s)
      平川智基、松岡淳一、諸橋賢吾、青野俊裕
    • Organizer
      日本土壌微生物学会2018年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] セスバニア根粒菌の宿主殺傷能に関わるrebオペロンの新たな発現制御因子群2016

    • Author(s)
      松岡淳一、青野俊裕
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      佐賀大学本庄キャンパス(佐賀県佐賀市)
    • Year and Date
      2016-09-20
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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