Holisitc search and functional analysis of transcription factors that regulate syringyl lignin biosynthesis
Project/Area Number |
16K07806
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Wood science
|
Research Institution | Kyoto University |
Principal Investigator |
Suzuki Shiro 京都大学, 生存圏研究所, 助教 (70437268)
|
Co-Investigator(Kenkyū-buntansha) |
光田 展隆 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (80450667)
|
Project Period (FY) |
2016-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | リグニン / 転写因子 / シリンギル / 酵母ワンハイブリッド / イネ / アセチル化 / アシル基転移酵素 / MYB / NAC / 酵母ワンハイブリッドスクリーニング |
Outline of Final Research Achievements |
Syringyl lignin is more easily degratative than other lignins, and monomerlized lignin can be obtained efficiently from syringyl lignin. Some plant species biosynthesize syringyl-rich lignin, which suggests the exsistence of transcriptional mechanism specific to syringyl lignin biosynthesis. In this research, we aim at holistic search of transcription factors involved in lignin biosynthesis using improved yeast one-hybrid screening (iY1H). As a result, we succeeded in finding the new series of transcription factors that bind to promoter regions of lignin biosynthetic enzymes.
|
Academic Significance and Societal Importance of the Research Achievements |
本研究は、近年確立された改良型酵母ワンハイブリッド法を用い、リグニン生合成を制御する転写因子を網羅的に探索することを目的とする。本研究により、リグニン合成酵素遺伝子のプロモーター領域に結合す一群の転写因子の同定に成功した。本研究により得られた転写因子の一群には、リグニン生合成を制御する転写因子であることが既に示されているMYB転写因子のほか、多くの新規転写因子が含まれていた。これらの転写因子を活用することにより、より分解が容易で、高付加価値利用が容易なリグニンを有するリグノセルロースバイオマスの作出につながると考えられる。
|
Report
(5 results)
Research Products
(48 results)
-
-
-
-
-
[Journal Article] Recruitment of specific flavonoid B-ring hydroxylases for two independent biosynthesis pathways of flavone-derived metabolites in grasses2019
Author(s)
Pui Ying Lam, Andy Lui, Masaomi Yamamura, Lanxiang Wang, Yuri Takeda, Shiro Suzuki, Hongjia Liu, Fu-Yuan Zhu, Mo-Xian Chen, Jian-Hua Zhang, Toshiaki Umezawa, Yuki Tobimatsu, Clive Lo
-
Journal Title
New Phytologist
Volume: 印刷中
Issue: 1
Pages: 2014-2019
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
[Journal Article] Downregulation of p‐COUMAROYL ESTER 3‐HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification2018
Author(s)
Yuri Takeda, Yuki Tobimatsu,Steven D. Karlen, Taichi Koshiba, Shiro Suzuki,Masaomi Yamamura, Shinya Murakami, Mai Mukai, Takefumi Hattori, Keishi Osakabe, John Ralph, Masahiro Sakamoto, Toshiaki Umezawa
-
Journal Title
Plant Journal
Volume: 95
Issue: 5
Pages: 796-811
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
[Journal Article] A comparative study of the biomass properties of Erianthus and sugarcane: lignocellulose structure, alkaline delignification rate, and enzymatic saccharification efficiency2018
Author(s)
Takuji Miyamoto, Masaomi Yamamura, Yuki Tobimatsu, Shiro Suzuki, Miho Kojima, Keiji Takabe, Yoshifumi Terajima, Asako Mihashi, Yoshinori Kobayashi, Toshiaki Umezawa
-
Journal Title
Bioschience, Biotechnology and Biohemistry
Volume: 82
Issue: 7
Pages: 1143-1152
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Regulation of CONIFERALDEHYDE 5-HYDROXYLASE expression to modulate cell wall lignin structure in rice2017
Author(s)
Takeda Y, Koshiba T, Tobimatsu Y, Suzuki S, Murakami S, Yamamura M, Rahman Md M, Takano T, Hattori T, Sakamoto M, Umezawa
-
Journal Title
Planta
Volume: 246
Issue: 2
Pages: 337-349
DOI
Related Report
Peer Reviewed
-
[Journal Article] MYB-mediated upregulation of lignin biosynthesis in <i>Oryza sativa</i> towards biomass refinery2017
Author(s)
Koshiba T, Yamamoto N, Tobimatsu Y, Yamamura M, Suzuki S, Hattori T, Mukai M, Noda S, Shibata D, Sakamoto M, Umezawa T
-
Journal Title
Plant Biotechnology
Volume: 34
Issue: 1
Pages: 7-15
DOI
NAID
ISSN
1342-4580, 1347-6114
Related Report
Peer Reviewed / Open Access
-
[Presentation] Altered cell wall chemical structure and molecular assembly in lignin-modified rice mutants2019
Author(s)
Andri Fadillah Martin, Yuki Tobimatsu, Pui Ying Lam, Naoyuki Matsumoto, Shiro Suzuki, Ryosuke Kusumi, Takuto Tanaka, Takuji Miyamoto, Yuri Takeda, Masaomi Yamamura, Taichi Koshiba, Keishi Osakabe, Yuriko Osakabe, Masahiro Sakamoto, Toshiaki Umezawa
Organizer
細胞壁研究者ネットワーク第13回定例研究会(岡崎)
Related Report
-
-
-
-
-
[Presentation] Lignin metabolic engineering in grasses for lignin valorization2019
Author(s)
Toshiaki Umezawa, Yuki Tobimatsu, Masaomi Yamamura, Masahiro Sakamoto, Shiro Suzuki, Yuri Takeda, Takuji Miyamoto, Taichi Koshiba, Rie Takada
Organizer
The 20th International Symposium on Wood, Fiber, and Pulping Chemistry
Related Report
Int'l Joint Research
-
[Presentation] Grass breeding toward lignin-enriched biomass via knockout of transcriptional repressors2019
Author(s)
Takuji Miyamoto, Rie Takada, Yuki Tobimatsu, Yuri Takeda, Shiro Suzuki, Masaomi Yamamura, Keishi Osakabe, Yuriko Osakabe, Masahiro Sakamoto, Toshiaki Umezawa
Organizer
The 20th International Symposium on Wood, Fiber, and Pulping Chemistry
Related Report
Int'l Joint Research
-
[Presentation] Pathway-specific flavonoid B-ring hydroxylases involved in the biosynthesis of flavone C-glycosides and tricin-lignins in rice2019
Author(s)
Pui Ying Lam, Andy C. W. Lui, Masaomi Yamamura, Lanxiang Wang, Yuri Takeda, Shiro Suzuki, Hongjia Liu, Fu-Yuan Zhu, Mo-Xian Chen, Jianhua Zhang, Toshiaki Umezawa, Yuki Tobimatsu, Clive Lo
Organizer
第37回日本植物細胞分子生物学会(京都)
Related Report
-
-
-
-
-
-
-
-
[Presentation] Lignin enrichment in grass biomass by molecular breeding2019
Author(s)
Takuji Miyamoto, Rie Takada, Yuri Takeda, Masaomi Yamamura, Yuki Tobimatsu, Shiro Suzuki, Wahyuni, Vincentia Esti Windiastri, Dwi Widyajayanti, Satya Nugroho, Masahiro Sakamoto, Toshiaki Umezawa
Organizer
4th SATREPS conference
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
[Presentation] Downregulation of p-COUMAROYL ESTER 3-HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification2018
Author(s)
Yuri Takeda, Yuki Tobimatsu, Steven D. Karlen, Taichi Koshiba, Shiro Suzuki, Masaomi Yamamura, Shinya Murakami, Mai Mukai, Takefumi Hattori, Keishi Osakabe, John Ralph, Masahiro Sakamoto, Toshiaki Umezawa
Organizer
The 3rd Asia Research Node Symposium on Humanosphere Science -Present and Future of Humanosphere Science - (National Chung Hsing University, Taichung, Taiwan)
Related Report
Int'l Joint Research
-
[Presentation] A bifunctional O-methyltransferase involved in the biosynthesis of flavonolignin in rice2018
Author(s)
Pui Ying Lam, Naoyuki Matsumoto, Shiro Suzuki, Wu Lan, Yuri Takeda, Masaomi Yamamura, Masahiro Sakamoto, Clive Lo, John Ralph, Yuki Tobimatsu, Toshiaki Umezawa
Organizer
第36回日本植物細胞分子生物学会
Related Report
-
-
-
[Presentation] Downregulation of p-COUMAROYL ESTER 3-HYDROXYLASE in rice leads to altered lignin structures and improves biomass usability2018
Author(s)
Yuri Takeda, Yuki Tobimatsu, Steven D. Karlen, Taichi Koshiba, Shiro Suzuki, Masaomi Yamamura, Shinya Murakami, Mai Mukai, Takefumi Hattori, Keishi Osakabe, John Ralph, Masahiro Sakamoto, Toshiaki Umezawa
Organizer
57th Annual Meeting of the Phytochemical Society of North America Autonomous University of San Luis Potosi (San Luis Potosi, Mexico)
Related Report
Int'l Joint Research
-
-
-
-
-
-