| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
The aim of this study was to establish the cryopreservation protocols suitable for tissue complexes composed of multiple cell types, such as bovine cumulus-oocyte complexes and rat pancreatic islets. Results obtained were: (1) Downsizing cumulus layers in germinal vesicle-stage bovine cumulus-oocyte complexes contributed to the higher revivability of the oocytes after Cryotop vitrification. (2) Large amounts of metaphase-II stage bovine oocytes were able to survive vitrification on a novel cryodevice, nylon mesh triangle. (3) Cryotop vitrification protocol was superior to conventional Bicell freezing regimen in cryopreservation of rat pancreatic islets, when their revivability was evaluated by glucose-stimulated insulin secretion ability in vitro. The nylon mesh triangle device was also applicable to a large amount of pancreatic islets. (4) Type-1 diabetes model rats were rescued from hyperglycemia by syngeneic transplantation of post-warm islets beneath the kidney capsule.
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