Analysis of luteal function using glycoprotein

• Project/Area Number: 16K07998
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Tokyo Metropolitan Industrial Technology Research Institute (2018-2019); Aomori Prefectural Industrial Technology Research Center (2016-2017)
• Principal Investigator: SANO MASAHIRO 地方独立行政法人東京都立産業技術研究センター, 開発本部開発第二部バイオ応用技術グループ, 研究員 (20645577)
• Co-Investigator(Kenkyū-buntansha): 山本 ゆき 岡山大学, 環境生命科学研究科, 助教 (20645345)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2016: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 黄体機能 / 糖タンパク質 / 黄体退行 / 糖鎖 / 細胞生存率 / 糖鎖機能 / ウシ発情周期 / プロテオグリカン / 分子量 / O-GlcNAc / OGT / OGA / フコース / シアル酸 / 黄体

Outline of Final Research Achievements

Alpha 2,6-sialylated glycoproteins inductively expressed by stimulation with PGF2α inhibits the interaction between galectin-1 and the N-glycoproteins on the cell surfaces, contribute to the regulation of luteolysis in cows. Furthermore, the level of core fucosylation was decreased at the regress stage, and the vaiability of luteal cells was decreased by fucosidase. The overall findings suggest that glycoproteins contribute to the regulation of luteal functions.

Academic Significance and Societal Importance of the Research Achievements

現在わが国ではプロスタグランジンF2αで発情誘起したウシの低受胎率が問題となっており、自然に近い形で黄体退行を誘起するための新規発情周期制御技術の開発が求められている。しかし、黄体機能の制御には様々な因子の関与することが知られているがその全容は未だ不明であり、更なる制御機構の解明が望まれている。本研究によって、糖鎖による新たな黄体制御機構が明らかになったことにより、これまでにはないアプローチによる黄体退行を誘起する新手法の開発の一助となることが考えられる。

Research Products

• [Journal Article] Expressions of lipoprotein receptors and cholesterol efflux regulatory proteins during luteolysis in bovine corpus luteum. (2017)
  • Author(s): Horihata K, Yoshioka S, Sano M, Yamamoto Y, Kimura K, Skarzynski DJ, Okuda K.
  • Journal Title: Reprod Fertil Dev. Volume: 29 Issue: 7 Pages: 1280-1286
  • DOI: 10.1071/rd15538
  • NAID: 120006349213
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Possible contribution of α2,6-sialylation to luteolysis in cows by inhibiting the luteotrophic effects of galectin-1 (2016)
  • Author(s): Hashiba K, Nio-Kobayashi J, Sano M, Maeda M, Kimura Y, Yamamoto Y, Kimura K, Okuda K
  • Journal Title: Biology of reproduction Volume: 95 (1) Issue: 1 Pages: 1-8
  • DOI: 10.1095/biolreprod.116.140194
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Expression Profiles and Possible Roles of Galectins in the Corpus Luteum (2016)
  • Author(s): Junko Nio-Kobayashi, Kazuhisa Hashiba, Masahiro Sano, Kiyoshi Okuda, W. Colin Duncan, Toshihiko Iwanaga
  • Journal Title: Trends in Glycoscience and Glycotechnology Volume: 28 Issue: 162 Pages: E71-E77
  • DOI: 10.4052/tigg.1416.1E
  • NAID: 130005165761
  • ISSN: 0915-7352, 1883-2113
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] High-Sensitivity and Low-Toxicity Fucose Probe for Glycan Imaging and Biomarker Discovery. (2016)
  • Author(s): Kizuka Y, Funayama S, Shogomori H, Nakano M, Nakajima K, Oka R, Kitazume S, Yamaguchi Y, Sano M, Korekane H, Hsu TL, Lee HY, Wong CH, Taniguchi N.
  • Journal Title: Cell Chemical Biology Volume: 23 Issue: 7 Pages: 782-792
  • DOI: 10.1016/j.chembiol.2016.06.010
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] 改変プロテオグリカンの機能性および構造解析 (2020)
  • Author(s): 佐野栄宏、成田武文
  • Organizer: 日本農芸化学会 2020年度福岡大会
• [Presentation] 黄体維持機構における Epidermal growth factor receptor の core fucosylation の役割 (2016)
  • Author(s): 羽柴 一久, 小林 純子, 佐野 栄宏, 前田 恵, 木村 吉伸, 奥田 潔, 木村 康二
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 麻布大学
  • Year and Date: 2016-09-11
• [Presentation] An increase in the level of α2,6-sialylation contributes to luteolysis in cows by inhibiting galectin-1 binding to glycan (2016)
  • Author(s): Kazuhisa Hashiba, Masahiro Sano, Junko Nio-Kobayashi, Megumi Maeda, Yoshinobu Kimura, Yuki Yamamoto, Koji Kimura, Kiyoshi Okuda
  • Organizer: Society for the Study of Reproduction
  • Place of Presentation: San Diego
  • Year and Date: 2016-07-16
  • Int'l Joint Research