Establishment of a novel monoclonal antibody production method for influenza treatment
| Project/Area Number |
16K08018
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Veterinary medical science
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
MURAMOTO Yukiko 京都大学, ウイルス・再生医科学研究所, 特別研究員(RPD) (70436567)
|
|---|
| Project Period (FY) |
2016-04-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | インフルエンザウイルス |
|---|
| Outline of Final Research Achievements |
Therapeutic antibodies utilizes the specificity of one monoclonal antibody to recognize one antigen. The aim of the study is to establish a method to establish monoclonal antibodies that can treat all influenza A virus infections. To that end, several methods of immunizing mice, several fusion methods, and some screening methods to detect antibody-producing hybridomas were performed, and finally, monoclonal antibodies which recognize multiple HA subtypes were established. That is, we have found a suitable method for producing antibodies that recognize multiple HA subtypes of influenza A virus. This method can also be applied to establish antibodies for the treatment of other viral infections.
|
| Academic Significance and Societal Importance of the Research Achievements |
抗体医薬品は、一つの抗体が一つの抗原を認識する特異性を利用した、副作用の少ない効果的な治療法として、がん、免疫炎症性疾患、感染症、その他の分野で利用されている。しかしインフルエンザの治療法としては未だ実用化されていない。インフルエンザウイルスの主要抗原HA蛋白質の抗原性がH1からH18までのHA亜型により、大きく異なることが大きな障害となっている。そこで本研究では、全てのHA亜型を認識する抗体の作出に適した方法を探索した。
|
Report
(4 results)
Research Products
(10 results)
