The attempt to improve the developmental competence of oocytes using in vitro growth model of bovine follicles and the application for the production of wildlife oocytes
| Project/Area Number |
16K08043
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Hokkaido University |
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Principal Investigator |
Nagano Masashi 北海道大学, 獣医学研究院, 准教授 (70312402)
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| Research Collaborator |
YANAGAWA Yojiro
SAKAGUCHI Kenichiro
KANNO Chihiro
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 卵子 / 体外受精 / 体外発育培養 / 細胞質成熟 / 卵胞 / 体外成熟培養 / 卵胞発育 |
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| Outline of Final Research Achievements |
To clarify the relationship between the steroidogenesis and the acquisition of developmental competence of bovine oocytes during follicular development or degradation, I cultured the developing bovine oocytes surrounded by granulosa cell layers. When FSH and BMP-4 were added to culture media of oocytes, luteinization of granulosa cells were inhibited but the developmental competence of oocytes decreased. Then I try to develop the new co-culture system with oocyte-granulosa cell complex (OGC) and theca cells which secrets several growth factors having the function for the development and the acquisition of developmental competence of oocytes. When OGC cultured with theca cells, theca and granulosa cells secreted androstenedione and estradiol-17β, respectively. And oocytes acquired the developmental competence to transferrable embryos. However, the developmental rate was low. We should develop the culture system which can support the function of theca and granulosa cells.
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| Academic Significance and Societal Importance of the Research Achievements |
体外受精は家畜生産およびヒトの不妊治療分野で広く用いられているが作製された胚の発生能は低い。本研究の成果は卵子が発生能を獲得する過程を体外で明らかにする培養系の開発につながり、発生能の高い胚の体外生産にも資する。すなわち、家畜生産効率や不妊治療成功率の向上につながり、絶滅の危機にある野生動物の増殖にも寄与できる。
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Effect of astaxanthin addition to an individual culture system for in vitro maturation of bovine oocytes on accumulation of reactive oxygen species and mitochondrial activity2018
Author(s)
Erdenetogtokh, P., Kanno, C., Sakaguchi, K., Yanagawa, Y., Katagiri, S. and Nagano, M
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Journal Title
Japanese Journal of Veterinary Research
Volume: 66
Pages: 325-329
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Extension of the culture period for the in vitro growth of bovine oocytes in the presence of bone morphogenetic protein-4 increases oocyte diameter, but impairs subsequent developmental competence2017
Author(s)
Yang, Y., Kanno, C., Sakaguchi, K., Yanagawa, Y., Katagiri, S. and Nagano, M.
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Journal Title
Animal Science Journal
Volume: 88
Issue: 11
Pages: 1686-1691
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Comparison of sperm subpopulation structures in first and second ejaculated semen from Japanese black bulls by a cluster analysis of sperm motility evaluated by a CASA system2017
Author(s)
Kanno, C., Sakamoto, K. Q., Yanagawa, Y., Takahashi, Y., Katagiri, S. and Nagano, M.
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Journal Title
Journal of Veterinary Medical Science
Volume: 79
Issue: 8
Pages: 1359-1365
DOI
NAID
ISSN
0916-7250, 1347-7439
Related Report
Peer Reviewed / Open Access
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[Journal Article] Extension of the culture period for the in vitro growth of bovine oocytes in the presence of bone morphogenetic protein-4 increases oocyte diameter, but impairs subsequent developmental competence2017
Author(s)
Yang, Y., Kanno, C., Sakaguchi, K., Yanagawa, Y., Katagiri, S., Nagano, M.*
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Journal Title
Animal Science Journal
Volume: 印刷中
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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