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Establishment of "Liquid Staging" using urine samples

Research Project

Project/Area Number 16K08045
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionThe University of Tokyo

Principal Investigator

Nakagawa Takayuki  東京大学, 大学院農学生命科学研究科(農学部), 准教授 (40447363)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsLiquid-biopsy / BRAF / vimentin / EMT / TCC / digital PCR / 移行上皮癌 / Liquid Staging / digitalPCR / イヌ / DigitalPCR / 癌 / 臨床 / 診断・検査
Outline of Final Research Achievements

The purpose of this study was to establish "Liquid Biopsy" that can diagnose and confirm the progress of transitional cell carcinoma of the dog, whose early definitive diagnosis is very difficult, from urine samples. For diagnosis, we established a Digital PCR method that can detect BRAF gene mutations, which are frequently found in canine transitional cell carcinoma, with high sensitivity, and succeeded in its clinical application. We investigated the development of the tumor by targeting epithelial-mesenchymal transition, and clarified that the mesenchymal marker vimentin is significantly associated with survival in canine transitional cell carcinoma. Based on these results, it was considered that the basic data of Liquid biopsy for tumor diagnosis and pathological condition grasping was formed by urine samples.

Academic Significance and Societal Importance of the Research Achievements

これまでの検査では犬移行上皮癌の早期診断は非常に困難であり、積極的な治療であっても生存期間は1年未満であった。本研究成果によって早期診断が実現したことで、犬移行上皮癌症例の生存予後は大きく改善することが期待されるだけでなく、尿サンプルを用いた"Liquid Biospy"の成功例の先駆けとなったと考える。また犬移行上皮癌においてvimentinが有意な予後指標となることを初めて見出したが、これも臨床応用可能な予後マーカーとなるだけでなく、浸潤性の高い犬移行上皮癌の分子生物学的な性状の解析やより効果的な治療法開発に向けた基盤的データとして意義ある成果と考えられた。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (8 results)

All 2018 2016

All Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Presentation] The role of ERK/MAPK pathway for epithelial-mesenchymal transition induction in canine urinary bladder transitional cell carcinoma.2018

    • Author(s)
      Shinada M, Saeki K, Yoshitake R, Eto S, Tuboi M, Chembers J, Uchida K, Kato D, Yoshimoto S, Ikeda N, Kamoto S, Nishimura R, Nakagawa T
    • Organizer
      8th Annual Congress of Asian Society of Veterinary Surgery
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ERK/MAPK経路は犬尿路上皮癌におけるCOX2/PGE2 Axisを活性化する2018

    • Author(s)
      吉竹涼平,衛藤翔太郎,品田真央,佐伯亘平,中野令,杉谷博士,西村亮平,中川貴之
    • Organizer
      第77回日本癌学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 犬尿路上皮癌における上皮間葉転換の評価とその予後との関連2018

    • Author(s)
      品田真央,佐伯亘平,吉竹涼平,衛藤翔太朗,加藤大貴,吉本翔,池田凡子,嘉本諭,坪井誠也,James Chambers,藤田直己,内田和幸,西村亮平,中川貴之
    • Organizer
      第161回日本獣医学会学術集会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 犬尿路上皮癌における上皮間葉転換の誘導の有無についての検討2018

    • Author(s)
      品田真央,佐伯亘平,吉竹涼平,衛藤翔太朗,西村亮平,中川 貴之
    • Organizer
      第18回東京大学生命科学シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] イヌ膀胱・前立腺移行上皮癌とBRAF遺伝子変異2018

    • Author(s)
      中川貴之
    • Organizer
      大阪府立大学獣医学専攻 第10回オープンセミナー
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] BRAFV595E変異犬尿路上皮癌におけるプロスタグランジンE2産生と細胞増殖を標的とした薬剤スクリーニング2018

    • Author(s)
      吉竹涼平、衛藤翔太郎、佐伯亘平、田中由依子、加藤大貴、吉本翔、西村亮平、中川貴之
    • Organizer
      先端モデル動物支援プラットフォーム 成果発表会2017
    • Related Report
      2017 Research-status Report
  • [Presentation] Impact of BRAF genotype on prognosis after total cystectomy in canine urinary bladder transitional cell carcinoma.2016

    • Author(s)
      Ryohei YOSHITAKE, Kohei SAEKI, Ryohei KINOSHITA, Kazuyuki UCHIDA, Takayuki NAKAGAWA, Ryohei NISHIMURA
    • Organizer
      6th Conference of Asian Society of Veterinary Surgery
    • Place of Presentation
      福岡国際会議場(福岡県、福岡市)
    • Year and Date
      2016-12-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Digital PCR-based BRAFV450E urine biopsy for urothelial cell carcinoma and prostate carcinoma.2016

    • Author(s)
      Kohei SAEKI, Takayuki NAKAGAWA, Ryohei NISHIMURA
    • Organizer
      第75回日本癌学会学術集会
    • Place of Presentation
      パシフィコ横浜(神奈川県、横浜市)
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2021-01-27  

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