Role of host immune response using bacterial components involved in protection against Salmonella infection

• Project/Area Number: 16K08080
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Integrative animal science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: Eguchi Masahiro 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, 上級研究員 (00312215)
• Co-Investigator(Kenkyū-buntansha): 小川 洋介 国立研究開発法人農業・食品産業技術総合研究機構, 動物衛生研究部門, 主任研究員 (10455305)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: サルモネラ / ワクチン / 感染防御 / 防御抗原 / 免疫応答

Outline of Final Research Achievements

The purpose of this study is to analyze bacterial components involved in protection against Salmonella infection and to contribute to new vaccine development. The bacterial components involved in the protection against Salmonella infection that we clarified that CD11b, CD11c, and Gr-1-positive cells increased at the early stage of infection. It is also possible that increased CD11b, CD11c and Gr-1 positive cells are involved in Salmonella exclusion. In addition, we also clarified that the expression of bacterial components involved in protection against Salmonella infection differs depending on the strain, and we believe that it can be used for epidemiological analysis of field isolates.

Academic Significance and Societal Importance of the Research Achievements

これまで、宿主内のサルモネラの排除機構は完全には解明されていない。本研究で得られたCD11b, CD11c, Gr-1陽性細胞が増加する知見は、サルモネラの排除機構解明の端緒につながるのではないかと考えている。また、株によって、感染防御に関与する菌体成分の発現が異なることが明らかとなり、誘導される感染防御能が株によって異なることが示唆された。得られた知見は、疫学的な解析を展開させることで、ワクチン開発に貢献できると考えている。
本研究で得られた知見は、家畜を用いた臨床試験やワクチン投与により惹起する免疫担当細胞の活性化に必要な免疫賦活剤の検討が可能となり、実用化に向けた工程に寄与できる。

Research Products

• [Journal Article] Identification of an Antigen Specific to a Protective Salmonella Monoclonal Antibody. (2019)
  • Author(s): Aribam SD, Masukagami Y, Shiraiwa K, Nishikawa S, Ogawa Y, Akiba M, Shimoji Y, Eguchi M.
  • Journal Title: Japan Agricultural Research Quarterly Volume: 53(3)
  • NAID: 130007706257
  • Peer Reviewed / Open Access
• [Journal Article] Determination of O:4 antigen-antibody affinity level in O:5 antigen positive and negative variants of Salmonella enterica serovar Typhimurium. (2017)
  • Author(s): Nakai Y, Ito A, Ogawa Y, Aribam SD, Elsheimer-Matulova M, Shiraiwa K, Kisaka SMB, Hikono H, Nishikawa S, Akiba M, Kawahara K, Shimoji Y, Eguchi M.
  • Journal Title: FEMS Microbiol Lett Volume: 367(7) Issue: 7
  • DOI: 10.1093/femsle/fnx062
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] The protective capacity of anti O:4 antigen antibodies against Salmonella infection is influenced by the presence or absence of O:5 antigen (2018)
  • Author(s): Marta ELSHEIMER-MATULOVA, Yumiko MASUKAGAMI, Yohsuke OGAWA, Yoshihiro SHIMOJI. Masahiro EGUCHI
  • Organizer: FEBS2018
  • Int'l Joint Research
• [Presentation] サルモネラO4抗体による感染防御機構 (2018)
  • Author(s): Marta Elsheimer-Matulova 、枡鏡優美子、白岩和真、西川明芳、小川洋介、下地善弘、江口正浩
  • Organizer: 第161回日本獣医学会学術集会
• [Presentation] サルモネラワクチンに向けた取り組み (2018)
  • Author(s): 江口正浩
  • Organizer: 第284回鶏病事例検討会
  • Invited
• [Presentation] Difference in O4 antibody protection against O5 antigen positive and negative of Salmonella (2018)
  • Author(s): Masahiro EGUCHI Marta ELSHEIMER-MATULOVA, Yumiko MASUKAGAMI, Kazumasa SHIRAIWA, Sayaka NISHIKAWA, Yohsuke OGAWA, Yoshihiro SHIMOJI
  • Organizer: 第91回日本細菌学会総会
• [Presentation] IgG and CD8 T cells are essential for protective immunity against Salmonella infection (2017)
  • Author(s): Masahiro Eguchi, Marta Elsheimer-Matulova, Swarmistha Aribam,, Yohsuke Ogawa,, Yoshihiro Shimoji
  • Organizer: IUMS 2017
  • Int'l Joint Research