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Analyses of functions in marine sulfur-oxidizing bacterium and their application to bioleaching

Research Project

Project/Area Number 16K08112
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionOkayama University

Principal Investigator

Kamimura Kazuo  岡山大学, 環境生命科学研究科, 教授 (80294445)

Research Collaborator Kanao Tadayoshi  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords硫黄酸化 / 硫黄酸化細菌 / バイオリーチング / チオ硫酸酸化 / 好酸性細菌 / 海洋性細菌 / 資源回収 / 海洋性 / 好酸性 / 硫黄代謝 / ゲノム解析 / チオ硫酸キノン酸化還元酵素 / チオ硫酸デヒドロゲナーゼ / 応用微生物学
Outline of Final Research Achievements

Halophilic acidophilic sulfur-oxidizing bacteria have gained increasing interest because of their importance in bioleaching operations in salt-containing environments. Acidithiobacillus sp. strain SH is a marine bacterium with sodium chloride-stimulated sulfur-, sulfite-, and thiosulfate-oxidizing activities. The clarification of RISCs oxidation mechanism is indispensable to develop the efficient bioleaching. A novel thiosulfate:quinone oxidoreductase (TQO) was purified and characterized from strain SH. The gene for TQO was determined in the draft genome sequence of strain SH. The draft genome of strain SH provided further insights into the genomic diversity of members of the genus Acidithiobacillus, enabled us to determine a gene encoding the novel TQO, and contributed to a better understanding of the mechanism for RISCs metabolism in sulfur-oxidizing prokaryotes.

Academic Significance and Societal Importance of the Research Achievements

海洋から単離した硫黄酸化細菌の硫黄代謝酵素やその遺伝子の解析によって、硫黄酸化細菌がこれまでに報告のないものであることが明らかとなるとともに、硫黄代謝に関与する酵素も新規であることが明らかとなった。この発見は、生物が硫黄代謝系をどのように進化させてきたかを理解する上で重要であるだけでなく、地球規模での硫黄の循環に果たす好酸性の海洋性硫黄酸化細菌の役割を理解する上でも学術的に重要である。また、好酸性・好塩性の硫黄酸化細菌は、バイオリーチングやバイオレメディエーションにも利用できるため、得られた成果は、金属資源の枯渇問題の解決や環境問題の解決に大きく貢献できると考えられ、その社会的意義は大きい。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (17 results)

All 2018 2017 2016 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (12 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Draft Genome Sequence of Acidithiobacillus sp. Strain SH, a Marine Acidophilic Sulfur-Oxidizing Bacterium.2018

    • Author(s)
      Kamimura K, Sharmin S, Yoshino E, Tokuhisa M, Kanao T.
    • Journal Title

      Genome Announcement

      Volume: 6 Issue: 6

    • DOI

      10.1128/genomea.01603-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Characterization of tetrathionate hydrolase from the marine acidophilic sulfur-oxidizing bacterium, Acidithiobacillus thiooxidans strain SH.2018

    • Author(s)
      Kanao T, Onishi M, Kajitani Y, Hashimoto Y, Toge T, Kikukawa H, Kamimura K.
    • Journal Title

      Bioscience, Biotechnology and Biochemistry

      Volume: 82 Issue: 1 Pages: 152-160

    • DOI

      10.1080/09168451.2017.1415128

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] 微生物を用いる湿式冶金―バイオリーチング―2017

    • Author(s)
      上村一雄、金尾忠芳
    • Journal Title

      生物工学

      Volume: 95 Pages: 662-666

    • Related Report
      2017 Research-status Report
    • Open Access
  • [Journal Article] 硫黄酸化微生物のエネルギー生成機構と環境・資源工学への利用2016

    • Author(s)
      上村一雄
    • Journal Title

      硫酸と工業

      Volume: 69 Pages: 133-145

    • NAID

      40020964173

    • Related Report
      2016 Research-status Report
  • [Presentation] Characterization of the novel thiosulfate:quinone oxidoreductase from a marine acidophilic sulfur-oxidizing bacterium, Acidithiobacillus sp. strain SH.2018

    • Author(s)
      Kazuo Kamimura, Sultana Sharmin, Eriko Yoshino, Mirai Tokuhisa, Tadayoshi Kanao
    • Organizer
      5th International Symposium on Microbial Sulfur Metabolism
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Characterization of tetrathionate hydrolase from the marine acidophilic sulfuroxidizing bacterium, Acidithiobacillus thiooxidans strain SH2018

    • Author(s)
      Tadayoshi Kanao
    • Organizer
      5th International Symposium on Microbial Sulfur Metabolism
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Acidithiobacillus ferrooxidansのチオ硫酸代謝に関する研究2018

    • Author(s)
      池田くみ子,金尾忠芳,上村一雄
    • Organizer
      日本農芸化学会2018年度中四国支部大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 化学合成独立栄養細菌の硫黄代謝の多様性2018

    • Author(s)
      上村一雄
    • Organizer
      日本農芸化学会中四国支部農芸化学若手セミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 海洋性硫黄酸化細菌Acidithiobacillus thiooxidans SH株のテトラチオン酸ハイドロラーゼに関する研究~第2報~2018

    • Author(s)
      金尾忠芳、上村一雄
    • Organizer
      日本農芸化学会中四国支部第50回記念講演会
    • Related Report
      2017 Research-status Report
  • [Presentation] 海洋性硫黄酸化細菌のチオ硫酸デヒドロゲナーゼの遺伝子解析2018

    • Author(s)
      上村一雄、徳久未来、金尾忠芳
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 鉄硫黄酸化細菌Acidithiobacillus ferrooxidans由来テトラチオン酸ハイドロラーゼの構造解析2018

    • Author(s)
      金尾忠芳、長谷成記、小坂恵、上村一雄、玉田太郎
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 鉄硫黄酸化細菌Acidithiobacillus ferrooxidans由来テトラチオン酸ハイドロラーゼの結晶構造解析2017

    • Author(s)
      長谷成記,中山久之,小坂恵,玉田太郎,上村一雄,金尾忠芳
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] チオ硫酸で生育した海洋性硫黄酸化細菌Acidithiobacillus thiooxidansの末端酸化酵素の性質2017

    • Author(s)
      吉野永里子,金尾忠芳,上村一雄
    • Organizer
      日本農芸化学会中四国支部第47回講演会
    • Place of Presentation
      島根大学(松江市)
    • Year and Date
      2017-01-28
    • Related Report
      2016 Research-status Report
  • [Presentation] A Novel Thiosulfate Dehydrogenase from a Marine Acidophilic Sulfur-Oxidizing Bacterium, Acidithiobacillus thiooxidans2017

    • Author(s)
      Kamimura K, Yoshino E, Tokuhisa M, Kanao T
    • Organizer
      15th International Congress of Bacteriology and Applied Microbiology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 海洋性A.thiooxidans SH株の末端酸化酵素の解析2016

    • Author(s)
      上村一雄,吉野永里子,金尾忠芳
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場(富山市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 鉄硫黄酸化細菌Acidithiobacillus ferrooxidans由来テトラチオン酸ハイドロラーゼの結晶構造解析2016

    • Author(s)
      長谷成記,中山久之,小坂恵,玉田太郎,上村一雄,金尾忠芳
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場(富山市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Research-status Report
  • [Remarks] 岡山大学農学部農芸化学コース微生物機能学分野紹介ページ

    • URL

      http://www.okayama-u.ac.jp/user/agr/profile/nougaku01_7.html

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2020-03-30  

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