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Robust genetic program for biological containment of environmentally invasive organisms

Research Project

Project/Area Number 16K08121
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Environmental agriculture(including landscape science)
Research InstitutionNational Agriculture and Food Research Organization

Principal Investigator

Kato Yusuke  国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門, ユニット長 (60214409)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords生物学的封じ込め / 非天然アミノ酸 / 合成生物学 / バイオセーフティ / バイオセキュリティ / 発現系
Outline of Final Research Achievements

Biological containment is a technique used to contain dangerous organisms in the laboratory. We proposed a novel containment system using a translational switch which is controlled by the naturally non-existent molecules, unnatural amino acids. Finally, we achieved a highly robust and reliable containment system with extremely low escape frequency (<1/1,000,000,000).

Academic Significance and Societal Importance of the Research Achievements

この方法を用いて、これまで不可能だった、組換え生物や、環境への影響が懸念される生物種を、開放環境中に放って利用できる可能性がある。たとえば、強力な汚染物質分解能を付与した組換え微生物による環境浄化、金属の酸化還元能力を強化した組換え微生物によるオンサイトの鉱物製錬、一定時間後に自然に死滅する生ワクチン、有害生物に対する病原体散布によるバイオコントロールなどが、有望である。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (19 results)

All 2020 2019 2018 2017 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (6 results)

  • [Journal Article] Extremely Low Leakage Expression Systems Using Dual Transcriptional-Translational Control for Toxic Protein Production2020

    • Author(s)
      Kato Yusuke
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 3 Pages: 705-705

    • DOI

      10.3390/ijms21030705

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Translational Control using an Expanded Genetic Code2019

    • Author(s)
      Yusuke Kato
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 20(4) Issue: 4 Pages: 887-887

    • DOI

      10.3390/ijms20040887

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tight Translational Control Using Site-Specific Unnatural Amino Acid Incorporation with Positive Feedback Gene Circuits2018

    • Author(s)
      Kato Yusuke
    • Journal Title

      ACS Synthetic Biology

      Volume: 7 Issue: 8 Pages: 1956-1963

    • DOI

      10.1021/acssynbio.8b00204

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 生態系を守る生物学的封じ込め技術2017

    • Author(s)
      加藤祐輔
    • Journal Title

      アグリバイオ

      Volume: 3 Pages: 32-37

    • Related Report
      2016 Research-status Report
  • [Presentation] 生物学的封じ込め技術-生ワクチンへの応用を例として-2020

    • Author(s)
      加藤祐輔
    • Organizer
      日本農芸化学会2020年度大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 病原性を人為的にコントロールできる高効率・高安全性生ワクチン2019

    • Author(s)
      加藤祐輔、宮澤光博、菅原和恵、長澤裕也、菊佳男、林智人
    • Organizer
      「細胞を創る」研究会12.0
    • Related Report
      2019 Annual Research Report
  • [Presentation] 病原性をコントロールできる生ワクチン株作出技術と大腸菌性乳房炎への適用の展望2019

    • Author(s)
      加藤祐輔、宮澤光博、菅原和恵、長澤裕也、菊佳男、林智人
    • Organizer
      第24回日本乳房炎研究会・学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 毒素―抗毒素系の多重化により堅牢にした非天然アミノ酸要求性による生物学的封じ込め法2019

    • Author(s)
      加藤祐輔
    • Organizer
      日本農芸化学会2019年度大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Highly-tight translational switch controlled by unnatural amino acidsTranslational control using an expanded genetic code2018

    • Author(s)
      Yusuke Kato
    • Organizer
      EMBO Workshop-Chemical Biology 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 部位特異的非天然アミノ酸導入を応用した厳密な翻訳調節法2018

    • Author(s)
      加藤祐輔
    • Organizer
      日本農芸化学会2018年度大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 正のフィードバック遺伝回路を用いて厳密に遺伝子の発現を制御する方法2017

    • Author(s)
      加藤祐輔
    • Organizer
      日本農芸化学会2017年度大会
    • Place of Presentation
      京都女子大学(京都府・京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Tight control of target gene expression using site-specific unnatural amino acid incorporation with positive-feedback gene circuits2017

    • Author(s)
      Yusuke KATO
    • Organizer
      The 7th International Meeting on Synthetic Biology, SB7.0
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 自然界にない物質がないと生きられない生物をつくる―生物学的封じ込め法―2017

    • Author(s)
      加藤祐輔
    • Organizer
      第69回日本生物工学会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Remarks] Researchmap( 加藤祐輔)

    • URL

      https://researchmap.jp/preastant4/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 農研機構 生体物質機能利用技術開発ユ ニ ッ ト

    • URL

      http://www.naro.affrc.go.jp/nias/introduction/chart/0205/index.html

    • Related Report
      2019 Annual Research Report 2018 Research-status Report 2017 Research-status Report
  • [Remarks] Researchmap( 加藤祐輔)

    • URL

      https://researchmap.jp/preastant4/

    • Related Report
      2018 Research-status Report
  • [Remarks] Researchmap(加藤祐輔)

    • URL

      https://researchmap.jp/preastant4/

    • Related Report
      2017 Research-status Report
  • [Remarks] 農研機構 生体物質機能利用技術開発ユニット

    • URL

      http://www.naro.affrc.go.jp/nias/introduction/chart/0205/index.html

    • Related Report
      2016 Research-status Report
  • [Remarks] PEGS Eurpoe 2014 "Difficult to Express Proteins"

    • URL

      http://www.pegsummiteurope.com/14/difficult-protein-expression/

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2022-12-28  

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