Development of innovative clinical analytical methods for next-generation drugs using DNA aptamers as molecular recognition elements
| Project/Area Number |
16K08200
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | University of Shizuoka |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
林 秀樹 岐阜薬科大学, 薬学部, 准教授 (00419665)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 抗体医薬 / DNAアプタマー / バイオアナリシス / アプタマー / HPLC / 分析科学 |
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| Outline of Final Research Achievements |
In this study, we developed simple and accurate bioanalytical methods for various therapeutic monoclonal antibodies (mAbs) using a nucleic acid molecule with a special three-dimensional structure called DNA aptamer as a molecular recognition element. Specifically, various DNA aptamers that selectively bind to therapeutic mAbs, such as the anticancer drug bevacizumab and pertuzumab, were obtained, and using them, the ELAA method, a multi-sample simultaneous analysis method, and the aptamer affinity purification - high temperature reversed-phase LC method, a high-precision HPLC analysis method, were developed. These methods are superior to conventional analytical methods in terms of simplicity and accuracy, and it has been verified that they are useful as clinical analytical methods.
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| Academic Significance and Societal Importance of the Research Achievements |
近年、抗体の機能を利用した抗体医薬ががんや関節リウマチの治療など汎用されているが、抗体医薬の効き目を正しく評価し、適切な治療へとつなげるためには、血中の抗体医薬濃度を正確に測定する必要がある。しかし、血中には抗体医薬と構造の非常によく似た抗体が数多く存在するため、その分析は容易ではなかった。我々はDNAアプタマーと呼ばれる特殊な立体構造を持つ核酸分子を分子認識素子として用い、種々の抗体医薬に対する簡便かつ正確な血中濃度分析法を開発しました。我々の分析法は、新薬やバイオシミラー開発期間の効率化、迅速な薬物治療効果判定や薬物投与計画策定などへの貢献が期待できる。
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] Bioanalysis of bevacizumab and infliximab by high-temperature reversed-phase liquid chromatography with fluorescence detection after immunoaffinity magnetic purification2016
Author(s)
K. Todoroki, T. Nakano, Y. Eda, K. Ohyama, H. Hayashi , D. Tsuji, J.Z. Min, K. Inoue, N. Iwamoto, A. Kawakami, Y. Ueki, K. Itoh, T. Toyo'oka
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Journal Title
Anal. Chim. Acta
Volume: 916
Pages: 112-119
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-Methylmor- pholinium Chloride as an Enantioseparation Enhancer for Chiral Derivatization-LC Analysis of D- and L-Amino acids2016
Author(s)
Kenichiro Todoroki, Misuzu Nakamura, Yuhi Sato, Kanoko Goto, Tatsuki Nakano, Yasuhiro Ishii, Jun Zhe Min, Koichi Inoue, Toshimasa Toyo'oka
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Journal Title
Chromatography
Volume: 37
Pages: 23-28
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Simultaneous and rapid determination of gefitinib, erlotinib and afatinib plasma levels using liquid chromatography/tandem mass spectrometry in patients with non-small-cell lung cancer2016
Author(s)
Hideki Hayashi, Yutaro Kita, Hirotoshi Iihara, Koumei Yanase, Yasushi Ohno, Chiemi Hirose, Maya Yamada, Kenichiro Todoroki, Kiyoyuki Kitaichi, Shinya Minatoguchi, Yoshinori Itoh, Tadashi Sugiyama
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Journal Title
Biomedical Chromatography
Volume: 30
Issue: 7
Pages: 1150-1154
DOI
Related Report
Peer Reviewed
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[Journal Article] Principal component analysis of molecularly-based signals from infant formula contaminations using LC-MS and NMR in foodomics.2016
Author(s)
Inoue, K., Tanada, C., Hosoya, T., Yoshida, S., Akiba, T., Min, J.Z., Todoroki, K., Yamano, Y., Kumazawa, S., Toyo'oka, T.
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Journal Title
J. Sci. Food Agric.
Volume: 96
Issue: 11
Pages: 3876-3881
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Diagnostic approach to breast cancer patients based on target metabolomics in saliva by liquid chromatography with tandem mass spectrometry.2016
Author(s)
Takayama T, Tsutsui H, Shimizu I, Toyama T, Yoshimoto N, Endo Y, Inoue K, Todoroki K, Min JZ, Mizuno H, Toyo'oka T.
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Journal Title
Clin Chim Acta.
Volume: 452
Pages: 18-26
DOI
Related Report
Peer Reviewed
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