Development of a sensitive localization method for protein phosphorylation sites based on MS/MS spectral simulation
| Project/Area Number |
16K08206
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Meijo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
妹尾 洋 愛知医科大学, 医学部, 教授 (50236113)
水野 智博 名城大学, 薬学部, 助教 (40711669)
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| Research Collaborator |
CORTHALS Garry
SUNI Veronika
ERO-UHLGREN Laura
HAAPANIEMI Pekka
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 質量分析 / プロテオミクス / リン酸化部位同定 / シミュレーション / プログラム開発 / タンパク質リン酸化 / スペクトルライブラリ |
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| Outline of Final Research Achievements |
We have developed a simulation program for MS/MS spectra of phosphopeptides. Although the original version of the program was based on Windows command prompt, the program was given an user interface and named SimPhospho version 1, which offers variable simulation parameters. The SimPhospho was evaluated on MS/MS spectra acquired using a TripleTOF mass spectrometer, and enabled phosphopeptide identification by spectral library searching using SpectraST software. Furthermore, simulation conditions were optimized for confident and sensitive localization of phosphorylation sites.
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| Academic Significance and Societal Importance of the Research Achievements |
現在、生体タンパク質の網羅的解析（プロテオミクス）を行うことにより一度に数万ものリン酸化が観察でき、それら膨大な報告は、PhosphoSitePlusなどのデータベースに次々と蓄積されている。本研究では、その検出感度をさらに高感度化したことにより、今まで困難であった低存在量リン酸化の観察を可能にすると期待できる。リン酸化は細胞内情報伝達の中心を担っており、様々な生体機能の制御に関わっていることから、それらリン酸化の網羅的解析は、今後、生命科学および医学・薬学のさらなる発展に貢献すると考えられる。
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Report
(4 results)
Research Products
(33 results)
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[Journal Article] Rules for PP2A-controlled phosphosignalling and drug responses2018
Author(s)
Otto Kauko, Susumu Y. Imanishi, Evgeny Kulesskiy, Teemu Daniel Laajala, Laxman Yetukuri, Anni Laine, Mikael Jumppanen, Pekka Haapaniemi, Luyao Ruan, Bhagwan Yadav, Veronika Suni, Taru Varila, Garry Corthals, Juri Reimand, Krister Wennerberg, Tero Aittokallio, Jukka Westermarck
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Journal Title
bioRxiv
Volume: -
Pages: 271841-271841
DOI
Related Report
Open Access / Int'l Joint Research
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[Journal Article] Phosphoproteomics to characterize host response during influenza A virus infection of human macrophages2016
Author(s)
Sandra Soderholm, Denis E. Kainov, Tiina Ohman, Oxana V. Denisova, Bert Schepens, Evgeny Kulesskiy, Susumu Y. Imanishi, Garry Corthals, Petteri Hintsanen, Tero Aittokallio, Xavier Saelens, Sampsa Matikainen, and Tuula A. Nyman
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Journal Title
Molecular & Cellular Proteomics
Volume: 15
Issue: 10
Pages: 3203-3219
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Phosphorylation of Notch1 by Pim kinases promotes oncogenic signaling in breast and prostate cancer cells2016
Author(s)
Niina M. Santio, Sebastian K.-J. Landor, Laura Vahtera, Jani Yla-Pelto, Elina Paloniemi, Susumu Y. Imanishi, Garry Corthals, Markku Varjosalo, Ganesh babu Manoharan, Asko Uri, Urban Lendahl, Cecilia Sahlgren, and Paivi Koskinen
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Journal Title
Oncotarget
Volume: 7
Issue: 28
Pages: 43220-43238
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Systemic map of protein phosphatase 2A (PP2A)-regulated phosphotargets and drug responses in cancer cells2017
Author(s)
Otto Kauko, Susumu Imanishi, Evgeny Kulesskiy, Teemu D. Laajala, Laxmana Yetukuri, Artur Padzik, Mikael Jumppanen, Pekka Haapaniemi, Bhagwan Yadaw, Veronika Suni, Taru Varila, Garry Corthals, Wennerberg Krister, Tero Aittokallio, and Jukka Westermarck
Organizer
AACR annual meeting 2017
Related Report
Int'l Joint Research
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