| Project/Area Number |
16K08363
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Medical pharmacy
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
武隈 洋 北海道大学, 薬学研究院, 准教授 (00396293)
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| Research Collaborator |
ISHIKAWA takehiko
KOSEKI chihiro
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | エンテロイド / トランスポーター / ｐ-糖タンパク質 / 小腸上皮細胞 / 代謝酵素 |
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| Outline of Final Research Achievements |
In this study, we aimed to establish a mass transport experiment system using an enteroid (small intestine tissue culture system) and apply it to the functional evaluation of efflux transporters. Uptake experiments confirmed that the abundance ratio of the substrate in the enteloid lumen by P-gp and Mrp2 was reduced in the primary enteroid by P-gp inhibitors, Mrp2 inhibitors and ATP production inhibitors. It has been shown that the efflux of the substrate by P-gp and Mrp2 can be measured using an enteroid, and this experimental system can be expected as a useful screening system to evaluate the influence of drugs on the transport function of efflux transporters.
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| Academic Significance and Societal Importance of the Research Achievements |
消化管吸収機構を明らかにするための既存の方法には、消化管内の生理的状態を保ったままin vitro系で評価する手法や、排出トランスポーターを簡便に評価する実験系が少ないことが問題点であった。小腸から作製されるエンテロイドは、消化管内の生理的状態を維持するため、有用な物質輸送解析のツールになる可能性がある。本研究の成果は、薬物吸収のメカニズムの解明や、薬物の吸収に及ぼす他の薬物あるいは食品成分の影響（相互作用）の解析に有用と考えられる。
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