Analyses of Cxxc5 functions during vaginal development

• Project/Area Number: 16K08436
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: Harada Masayo 東京医科歯科大学, 大学院医歯学総合研究科, 助教 (80555756)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 膣 / 中腎傍管 / Cxxc5 / 中腎管 / 解剖学 / 細胞・組織 / 遺伝学

Outline of Final Research Achievements

Congenital vaginal defects are the causes of infertility. Many cases of vaginal defects have been reported, but there is little study about the pathological analyses of congenital vaginal defects and the mechanisms of vaginal development. The present study aimed to clarify the molecular mechanisms involving Cxxc5 of vaginal development. Cxxc5 was expressed in Mullerian ducts and Wolffian ducts. The current study revealed that Cxxc5 in Mullerian ducts are necessary for the extension of the lower end of Mullerian ducts which become vagina.

Academic Significance and Societal Importance of the Research Achievements

先天性膣欠損症の発症機序について、左右中腎傍管の伸長途中停止により左右の中腎傍管が中央で合せずに膣が形成されず開口しないことを明らかにした。また、先天性膣欠損症の一因がCxxc5の機能欠失である可能性を示した。

Research Products

• [Journal Article] Spatial distribution of smooth muscle tissue in the male pelvic floor with special reference to the lateral extent of the rectourethralis muscle: Application to prostatectomy and proctectomy (2018)
  • Author(s): Muro Satoru、Tsukada Yuichiro、Harada Masayo、Ito Masaaki、Akita Keiichi
  • Journal Title: Clinical Anatomy Volume: 31 Issue: 8 Pages: 1167-1176
  • DOI: 10.1002/ca.23254
  • Peer Reviewed
• [Journal Article] A mutation in transcription factor MAFB causes Focal Segmental Glomerulosclerosis with Duane Retraction Syndrome (2018)
  • Author(s): Sato Yoshinori、Tsukaguchi Hiroyasu、Morita Hiroyuki、略 & Takahashi Satoru、Yoshimura Ashio
  • Journal Title: Kidney International Volume: 94 Issue: 2 Pages: 396
  • DOI: 10.1016/j.kint.2018.02.025
  • Peer Reviewed
• [Presentation] The N143T mutation in mouse Fibroblast growth factor 9 leads to wider long bones (2018)
  • Author(s): Masayo Harada, Keiich Akita
  • Organizer: Joint Annual Meeting of JSDB 51st and JSCB 70th
• [Presentation] Muscle layer structure inanterior wall of anorectal canal in females (2018)
  • Author(s): Satoru Muro, Yuichiro Tsukada, Masayo Harada, Masaaki Ito, Keiichi Akita
  • Organizer: 35th Annual meeting of American Association of Clinical Anatomy
  • Int'l Joint Research
• [Presentation] マウス線維芽細胞増殖因子9のN143T変異は長管骨を太くする (2018)
  • Author(s): 原田理代、秋田恵一
  • Organizer: 第36回日本骨代謝学会学術集会
• [Presentation] マウス線維芽細胞増殖因子9 のN143T変異は長管骨を太くする (2018)
  • Author(s): 原田理代、秋田恵一
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] 女性の直腸肛門管前壁における筋層構造の解剖 輪走筋（内肛門括約筋）と縦走筋の収斂と前方突出 (2018)
  • Author(s): 室生暁、塚田祐一郎、原田理代、伊藤雅昭、秋田恵一
  • Organizer: 第28回骨盤外科機能温存研究会
• [Presentation] 直腸肛門管前方における平滑筋組織の三次元的な広がり (2018)
  • Author(s): 室生 暁、塚田 祐一郎、那須 久代、原田 理代、山口 久美子、伊藤 雅昭、秋田 恵一
  • Organizer: 第123回日本解剖学会
• [Presentation] The spontaneous mutation (N143T) in mouse Fgf9 leads to thicker long bones (2017)
  • Author(s): Masayo Harada、 Keiichi Akita
  • Organizer: 14th International Conference on Limb Development and Regeneration 2017
  • Int'l Joint Research
• [Presentation] 男性肛門管前方領域における平滑筋組織の分布－直腸尿道筋とその延長構造－ (2017)
  • Author(s): 室生 暁、塚田 祐一郎、那須 久代、原田 理代、秋田 恵一
  • Organizer: 第21回臨床解剖研究会
• [Presentation] Structural analysis of the anterior and posterior regions of the external anal sphincter; the spatial relationship with the bulbospongiosus and the coccyx. (2016)
  • Author(s): Satoru Muro, Phichaya Baramee, Janyaruk Suriyut, Hisayo Nasu, Masayo Harada, Kumiko Yamaguchi, Keiichi Akita.
  • Organizer: 8th International Symposium of Clinical and Applied Anatomy
  • Place of Presentation: Budapest (Hungary)
  • Int'l Joint Research