| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
I found that both N and PUB domains, especially the PUB domain, of UBXN-6 are important for the interaction with CDC-48. In the mutant worms containing the PUB-domain-lacking UBXN-6, mutant UBXN-6 was not detected by Western blotting. Interestingly, it was however detected when the worms were treated with the proteasome inhibitor. These results suggested that the PUB-domain-lacking UBXN-6 can not properly interact with CDC-48 and free UBXN-6 molecules were degraded by the proteasome. This notion strongly supports the Clip model. I also found that the amount of UBXN-6 was increased upon starvation and that UBXN-6 is involved in the formation of late endosomes.
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