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MicroRNA expression profile correlated with early colorectal cancer with lymph node metastasis

Research Project

Project/Area Number 16K08694
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Human pathology
Research InstitutionYokohama City University

Principal Investigator

Tateishi Yoko  横浜市立大学, 医学部, 助教 (20644438)

Co-Investigator(Kenkyū-buntansha) 奥寺 康司  横浜市立大学, 医学部, 准教授 (10326027)
梅田 茂明  横浜市立大学, 附属病院, 助教 (30644439)
稲山 嘉明  横浜市立大学, 附属市民総合医療センター, 教授 (10184730)
大橋 健一  横浜市立大学, 医学研究科, 教授 (40231203)
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords早期大腸癌 / リンパ節転移 / microRNA / 網羅的発現解析 / 網羅的解析
Outline of Final Research Achievements

The purpose of this study is to identify the microRNAs (miRNA) expression profile in early (submucosal invasive) colorectal cancer (CRC) with lymph node metastasis. In two early CRC cases, the primary CRC elements and the lymph node metastasis elements were separately collected from formalin fixed paraffin embedded (FFPE) specimens by laser microdissection system. Total RNAs were extracted and then subjected to comprehensive miRNA expression analyses (Agilent Expression Array). Differentially expressed miRNAs in the lymph node metastasis elements, whose levels showed more than two folds changes in compared to those of the primary CRC elements, were picked up. miR-125b was obtained as the upregulated miRNA, while eight miRNAs including miR-1228 were obtained as the downregulated miRNAs.

Academic Significance and Societal Importance of the Research Achievements

早期大腸癌は多くが内視鏡切除により根治できるが、約10%にリンパ節転移を伴い外科切除が必要となる。このような高悪性度病変の峻別は早期大腸癌の病理診断における重要な課題である。本研究では、リンパ節転移を伴う早期大腸癌の原発巣とリンパ転移巣のmicroRNA発現プロファイルを明らかにする。早期大腸癌の高悪性度病変を峻別する分子指標の同定することで、悪性度を予測するバイオマーカーとして臨床への応用が期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (4 results)

All 2018 2017 2016

All Presentation (4 results) (of which Int'l Joint Research: 1 results)

  • [Presentation] 早期大腸癌のリンパ節転移に特徴的なmicroRNA発現解析プロファイルの解明2018

    • Author(s)
      立石陽子
    • Organizer
      第107回日本病理学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] MicroRNA expression profile correlated with lymph node metastasis in early colorectal cancer2018

    • Author(s)
      立石陽子
    • Organizer
      第77回日本癌学会学術総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Expressions of oncogenic modulator microRNA-31and microRNA-21 are up-regulated in early colorectal cancer2017

    • Author(s)
      立石陽子
    • Organizer
      Digestive disease week 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] miR-31およびmiR-21は早期大腸癌において発現が亢進している2016

    • Author(s)
      立石陽子
    • Organizer
      第105回日本病理学会総会
    • Place of Presentation
      仙台国際センター会議場(宮城県)
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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